The importance of exportin and Ran for nucleocytoplasmic shuttling of the ecdysteroid receptor

Betanska, Katarzyna; Honl, Celine; Spindler-Barth, Margarethe; Spindler, Klaus-Dieter

doi:10.1002/arch.20397

Cited by 2 publications

(5 citation statements)

References 31 publications

(48 reference statements)

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“…Therefore, we infer that the daily nucleocytoplasmic shuttling of EcR that occurs during defined periods of larval–adult development of Rhodnius employs cytoskeletal tracks for movement. Nucleocytoplasmic shuttling of EcR is further supported by the fact that EcRs in other species possess several nuclear export signals in addition to a nuclear import signal (Gwózdz et al, 2007; Betanska et al, 2011). Because the nuclear EcR rhythm is synchronous to the robust circadian rhythm of circulating ecdysteroids it is inferred that Rhodnius cells detect and respond to the daily signals in the rhythmic ecdysteroid titer.…”

Section: Discussionmentioning

confidence: 99%

“…In mammals, steroid receptors and most of the nuclear factors involved in signaling cascades are continually shuttling between the nucleus and the cytoplasm (review by Echeverria and Picard, 2010). EcR possesses both nuclear localization and nuclear export signals (Gwózdz et al, 2007) and interacts with exportin 1 (Betanska et al, 2011). The presence of cytoplasmic EcR in insects has been noted but no significance has been attributed to it (Lammerding-Köppel et al, 1998; Gwózdz et al, 2007; Nieva et al, 2007; Betanska et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…EcR possesses both nuclear localization and nuclear export signals (Gwózdz et al, 2007) and interacts with exportin 1 (Betanska et al, 2011). The presence of cytoplasmic EcR in insects has been noted but no significance has been attributed to it (Lammerding-Köppel et al, 1998; Gwózdz et al, 2007; Nieva et al, 2007; Betanska et al, 2011). The only evidence that EcR undergoes shuttling derives from evidence in Rhodnius prolixus (Vafopoulou and Steel, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Cytoplasmic travels of the ecdysteroid receptor in target cells: pathways for both genomic and non-genomic actions

Vafopoulou

Steel

2012

Front. Endocrin.

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Signal transduction of the insect steroid hormones, ecdysteroids, is mediated by the ecdysteroid receptor, EcR. In various cells of the insect Rhodnius prolixus, EcR is present in both the nucleus and the cytoplasm, where it undergoes daily cycling in abundance and cellular location at particular developmental times of the last larval instar that are specific to different cell types. EcR favors a cytoplasmic location in the day and a nuclear location in the night. This study is the first to examine the potential mechanisms of intracellular transport of EcR and reveals close similarities with some of its mammalian counterparts. In double and triple labels using several antibodies, immunohistochemistry, and confocal laser scanning microscopy, we observed co-localization of EcR with the microtubules (MTs). Treatments with either the MT-stabilizing agent taxol or with colchicine, which depolymerizes MTs, resulted in considerable reduction in nuclear EcR with a concomitant increase in cytoplasmic EcR suggesting that MT disruption inhibits receptor accumulation in the nucleus. EcR also co-localizes with the chaperone Hsp90, the immunophilin FKBP52, and the light chain 1 of the motor protein dynein. All these factors also co-localize with MTs. We propose that in Rhodnius, EcR exerts its genomic effects by forming a complex with Hsp90 and FKBP52, which uses dynein on MTs as a mechanism for daily nucleocytoplasmic shuttling. The complex is transported intact to the nucleus and dissociates within it. We propose that EcR utilizes the cytoskeletal tracks for movement in a manner closely similar to that used by the glucocorticoid receptor. We also observed co-localization of EcR with mitochondria which suggests that EcR, like its mammalian counterparts, may be involved in the coordination of non-genomic responses of ecdysteroids in mitochondria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytoplasmic travels of the ecdysteroid receptor in target cells: pathways for both genomic and non-genomic actions

Vafopoulou

Steel

2012

Front. Endocrin.

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“…Current studies have not yet clarify whether the TOR pathway mediates the Rho1 effect on egg development and production in wasps. However, it has been shown that knockdown of Ras superfamily member, Ran causes ecdysteroid receptor arrest in the cytoplasm (He et al, 2010;Betanska et al, 2011) or downregulation of genes responsible for ecdysteroid biosynthesis (Liu et al, 2021). This study, measured ecdysteroid titers in females and observed that MmRho1 knockdown significantly increased (Fig.…”

Section: Discussionmentioning

confidence: 73%

“…, 2010; Betanska et al. , 2011) or downregulation of genes responsible for ecdysteroid biosynthesis (Liu et al. , 2021).…”

Section: Discussionmentioning

confidence: 99%

Rho 1 participates in parasitoid wasp eggs maturation and host cellular immunity inhibition

et al. 2022

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Endoparasitoid wasps introduce venom into their host insects during the egglaying stage. Venom proteins play various roles in the host physiology, development, immunity, and behavior manipulation and regulation. In this study, we identified a venom protein, MmRho1, a small guanine nucleotide-binding protein derived from ovary in the endoparasitoid wasp Microplitis mediator and found that knockdown of its expression by RNA interference caused down-regulation of vitellogenin and juvenile hormone, egg production, and cocoons formation in the female wasps. We demonstrated that MmRho1 entered the cotton bollworm's (host) hemocytes and suppressed cellular immune responses after parasitism using immunofluorescence staining. Furthermore, wasp MmRho1 interacted with the cotton bollworm's actin cytoskeleton rearrangement regulator diaphanous by yeast 2-hybrid and glutathione s-transferase pull-down. In conclusion, this study indicates that MmRho1 plays dual roles in wasp development and the suppression of the host insect cellular immune responses.

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The importance of exportin and Ran for nucleocytoplasmic shuttling of the ecdysteroid receptor

Cited by 2 publications

References 31 publications

Cytoplasmic travels of the ecdysteroid receptor in target cells: pathways for both genomic and non-genomic actions

Cytoplasmic travels of the ecdysteroid receptor in target cells: pathways for both genomic and non-genomic actions

Rho 1 participates in parasitoid wasp eggs maturation and host cellular immunity inhibition

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