The imperial coach of D. Pedro II: DNA fungal identification in selected materials and its relation to biodeterioration and aerobiology

Abstract

The imperial coach of emperor D. Pedro II was built by Pearce & Countz British Company, responsible for the coachs from the British Monarchy, for his crowning ceremony, in 1841. Its component materials are of several natures, with distinct mechanical strength and some incompatibilities with environmental patterns in its place of storage. Specific parts, such as corduroy fabrics, ornaments and horse manes were investigated for microorganisms present both in the interior and exterior of the Imperial Coach. From this monitoring, 37 filamentous fungi, 6 yeasts and 4 bacterial strains were isolated (DNA sequencing). Some of them are known for their toxic and pathogenic nature. Most microbes found can damage its constituting materials.The imperial coach of D. Pedro II: DNA fungal identification in selected materials and its relation to biodeterioration and aerobiology Palavras-chave Berlinda Biodeterioração Biologia molecular ResumoA berlinda de aparato do imperador D. Pedro II foi construída pela companhia britânica Pearce & Countz, responsável pela construção das carruagens da monarquia britânica, para sua cerimônia de coroação, em 1841. Seus materiais componentes são de diferentes naturezas, com distintas resistências mecânicas e algumas incompatibilidades com padrões ambientais de sua área de guarda. Partes específicas, tais como tecidos de algodão, ornamentos e crinas de cavalo foram investigadas, bem como a microbiota presente no interior e no exterior da berlinda imperial. A partir deste monitoramento, 37 colônias de fungos filamentosos, 6 leveduras e 4 bactérias foram isoladas (sequenciamento do DNA). A maioria dos micro-organismos pode danificar os materiais constituintes.

Ecology and identification of environmental fungi and metabolic processes involved in the biodeterioration of Brazilian soapstone historical monuments

“…have been found in wood derived from the imperial coach of Pedro II of the 19th century (Lutterbach et al . ).…”

Assessment of acid mist on mortar biodeterioration simulating the wall of Jardim da Princesa, the National Museum of Rio de Janeiro, Brazil

“…Regrettably, little attention has been devoted to the biodeterioration effects on Brazilian heritage monuments. Brazil, a 520-year-old country, own many historical monuments and cities that are strengthened by biodeterioration, particularly context of global changes (Gaylarde et al, 2018;Shirakawa et al, 2013;Lutterbach et al, 2013). The present work aimed to evaluate the biodeterioration process on material similar to that found in the walls of the Jardim da Princesa, located in the National Museum of Rio de Janeiro (NM).…”