The immunomodulatory effect of vitamin D in chickens is dose-dependent and influenced by calcium and phosphorus levels

Rodríguez-Lecompte, J. C.; Yitbarek, Alexander; Cuperus, Tryntsje; Echeverry, H.; Dijk, Albert van

doi:10.3382/ps/pew186

Cited by 41 publications

(37 citation statements)

References 35 publications

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“…However, the immunomodulatory, anti-inflammatory and anti-coccidia roles of VD 3 or its metabolites have also been demonstrated in chickens [ 21 – 24 ] and chicken cells [ 23 , 25 ]. In addition, Rodriguez-Lecompte et al [ 26 ] reported that vitamin D or 25(OH)D, both have a robust immunomodulatory property with a more favorable helper T cell (Th2) response in broiler chickens. Aslam et al (1998) [ 27 ] observed that vitamin D deficiency depresses the cellular immune responses in young broiler chicks.…”

Section: Introductionmentioning

confidence: 99%

Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress

Geng

Wang

et al. 2018

Nutr Metab (Lond)

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BackgroundThe effects of vitamin D on the immune function of laying hens are not well understood. This study investigated the effects of vitamin D3 (VD3) on laying performance and immunological functions in laying hens under Escherichia coli lipopolysaccharide (LPS) challenge.MethodsIn experiment one, 360 Jinghong-1 strain layers (32 weeks) were randomly divided into four groups with six replicates per group and 15 hens per replicate. Hens were fed a basal diet supplemented with different levels of VD3 (0; 500; 1500; or 3000 IU VD3/kg of diet) for 10 weeks to determine laying performance, egg quality, and other parameters. In experiment two, 24 Jinghong laying hens (32 weeks) were fed basal diets with either 0 or 3000 IU VD3/kg of diet. After 10 weeks of feeding, six hens from each treatment were injected intravenously with 8 mg/kg of body weight of either LPS or saline. Blood and spleen samples were obtained for immune parameter analysis 4 h after injection.ResultsVD3 deficiency reduced egg production and egg quality; in addition, feed intake and feed-to-egg ratio increased. No significant differences were observed in these parameters except eggshell strength between dietary VD3 supplemental levels at 500; 1500; and 3000 IU VD3/kg of diet. VD3 deficiency increased serum hormone (calcitonin, parathyroid hormone, estradiol, and progesterone) and cytokine (IL-6, IL-10) levels, the ratio of IFN-γ to IL-4, myeloperoxidase activity and total IgG content in the serum, and upregulated the blood CD3+ T cell population. Splenic retinoid X receptor (RXR), nuclear factor-κB (NF-κB), inducible nitric oxide synthase (iNOS), and polymeric immunoglobulin receptor (pIgR) gene mRNA levels were upregulated in VD3-deficienct hens. VD3 deficiency significantly reduced serum Follicle stimulating hormone (FSH) and Luteinizing hormone (LH) concentrations and the number of CD4+CD25+ T cells in the blood. These changes were completely normalized by VD3 sufficiency. LPS reduced serum LH concentration, splenic lysozyme, and pIgR gene mRNA levels. LPS induced an increase in total serum IgM levels and the percentage of CD8+ T cells in the blood. The changes were completely reversed by VD3 addition.ConclusionVD3 supplementation could protect laying hens not only from VD3 deficiency but also from immunological stress.

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Section: Introductionmentioning

confidence: 99%

Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress

Geng

Wang

et al. 2018

Nutr Metab (Lond)

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“…In poultry, 20 and 200 ng/mL (equivalent to 48 and 480 nmol/L) vitamin D 3 has been shown to induce AvBD3, AvBD6, and AvBD9 in chicken embryonic intestinal epithelial cells, while AvBD1, AvBD6, and AvBD9 were induced in a dose-dependent manner in primary monocytes ( Zhang et al., 2016 ). Consistently, CATH-1 and CATH-B1 were dose-dependently induced by dietary vitamin D in the spleen of chickens and that induction was further enhanced by low calcium and phosphorus in the diet, although CATH-3 was suppressed ( Rodriguez-Lecompte et al., 2016 ). However, only CATH-1 was significantly induced in response to 9,800 IU/kg of vitamin D 3 supplementation in the bursa of Fabricius, while no cathelicidins were enhanced by any of the treatments in peripheral blood mononuclear cells ( Rodriguez-Lecompte et al., 2016 ).…”

Section: Impact Of Vitamin D On Hdp Expressionmentioning

confidence: 89%

“…Consistently, CATH-1 and CATH-B1 were dose-dependently induced by dietary vitamin D in the spleen of chickens and that induction was further enhanced by low calcium and phosphorus in the diet, although CATH-3 was suppressed ( Rodriguez-Lecompte et al., 2016 ). However, only CATH-1 was significantly induced in response to 9,800 IU/kg of vitamin D 3 supplementation in the bursa of Fabricius, while no cathelicidins were enhanced by any of the treatments in peripheral blood mononuclear cells ( Rodriguez-Lecompte et al., 2016 ). Intraperitoneal injection of chickens with 5,000 IU/kg of body weight of vitamin D failed to upregulate HDP expression in the chicken intestinal tract, but multiple HDP were synergistically induced in response to both vitamin D and LPS ( Lu et al., 2015 ).…”

Section: Impact Of Vitamin D On Hdp Expressionmentioning

confidence: 89%

Dietary modulation of endogenous host defense peptide synthesis as an alternative approach to in-feed antibiotics

Robinson

Liu

et al. 2018

Animal Nutrition

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Traditionally, antibiotics are included in animal feed at subtherapeutic levels for growth promotion and disease prevention. However, recent links between in-feed antibiotics and a rise in antibiotic-resistant pathogens have led to a ban of all antibiotics in livestock production by the European Union in January 2006 and a removal of medically important antibiotics in animal feeds in the United States in January 2017. An urgent need arises for antibiotic alternatives capable of maintaining animal health and productivity without triggering antimicrobial resistance. Host defense peptides (HDP) are a critical component of the animal innate immune system with direct antimicrobial and immunomodulatory activities. While in-feed supplementation of recombinant or synthetic HDP appears to be effective in maintaining animal performance and alleviating clinical symptoms in the context of disease, dietary modulation of the synthesis of endogenous host defense peptides has emerged as a cost-effective, antibiotic-alternative approach to disease control and prevention. Several different classes of small-molecule compounds have been found capable of promoting HDP synthesis. Among the most efficacious compounds are butyrate and vitamin D. Moreover, butyrate and vitamin D synergize with each other in enhancing HDP synthesis. This review will focus on the regulation of HDP synthesis by butyrate and vitamin D in humans, chickens, pigs, and cattle and argue for potential application of HDP-inducing compounds in antibiotic-free livestock production.

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“…However, CATH-B1 gene expression seems not restricted to the bursa of Fabricius. Although at much lower levels than found in the bursa, CATH-B1 mRNA was present in several tissues, including spleen and multiple segments of the respiratory and gastrointestinal tract of chicken (Achanta et al, 2012;Rodriguez-Lecompte et al, 2016). CATH-B1 gene expression was also observed in chicken HD11 macrophages and primary monocytes (Sunkara, 2011).…”

Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Chicken Cathelicidin B1 Against Influenza A Virus

Peng

Balhuizen

et al. 2020

Front. Microbiol.

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Cathelicidins (CATHs) are host defense peptides (HDPs) that play an important role in the innate immune response against infections. Although multiple functions of cathelicidins have been described, including direct antimicrobial activity and several immunomodulatory effects on the host, relatively little is known about their antiviral activity. Therefore, in vitro antiviral activity of chicken cathelicidins and the underlying mechanism was investigated in this study against different influenza A virus (IAV) strains. Our results show that chicken CATH-B1 has broad anti-IAV activity compared to other cathelicidins (CATH-1,-2,-3, LL-37, PMAP-23, and K9CATH) with an inhibition of viral infection up to 80% against three tested IAV strains (H1N1, H3N1, and H5N1). In agreement herewith, CATH-B1 affected virus-induced inflammatory cytokines expression (IFN-β, IL-1β, IL-6, and IL-8). Incubation of cells with CATH-B1 prior to or after their inoculation with virus did not reduce viral infection indicating that direct interaction of virus with the peptide was required for CATH-B1's antiviral activity. Experiments using combined size exclusion and affinity-based separation of virus and peptide also indicated that CATH-B1 bound to viral particles. In addition, using electron microscopy, no morphological change of the virus itself was seen upon incubation with CATH-B1 but large aggregates of CATH-B1 and viral particles were observed, indicating that aggregation might be the mechanism of action reducing IAV infectivity. Neuraminidase (NA) activity assays using monovalent or multivalent substrates, indicated that CATH-B1 did not affect NA activity per se, but negatively affected the ability of virus particles to interact with multivalent receptors, presumably by interfering with hemagglutinin activity. In conclusion, our results show CATH-B1 has good antiviral activity against IAV by binding to the viral particle and thereby blocking viral entry.

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The immunomodulatory effect of vitamin D in chickens is dose-dependent and influenced by calcium and phosphorus levels

Cited by 41 publications

References 35 publications

Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress

Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress

Dietary modulation of endogenous host defense peptide synthesis as an alternative approach to in-feed antibiotics

Antiviral Activity of Chicken Cathelicidin B1 Against Influenza A Virus

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