The immunomodulatory activity of Chenopodium ambrosioides reduces the parasite burden and hepatic granulomatous inflammation in Schistosoma mansoni-infection

Rodrigues, João Gustavo Mendes; Albuquerque, Paula Sibelly Veras; Nascimento, Johnny Ramos do; Campos, Jaianna Andressa Viana; Godinho, Andressa S S; Araújo, Sulayne Janayna; Brito, Jéfferson Mesquita; Jesus, Caroline M; Miranda, Guilherme Silva; Rezende, Michelle Carvalho de; Negrão-Corrêa, Déborah; Rocha, Cláudia Quintino da; Silva, Lucilene Amorim; Guerra, Rosane Nassar Meireles; Nascimento, Flávia R.F.

doi:10.1016/j.jep.2020.113287

Cited by 12 publications

(13 citation statements)

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“…The plant material was collected, cleaned, dried and powdered. The powder was macerated with a 70% hydroethanolic solution, a traditional extraction used in Brazilian folk medicine (Rodrigues et al ., 2020) and mixed every 12 h for a total of 48 h at a ratio of 1:5 (w/v). The extract was first filtered five times and then concentrated under reduced pressure to obtain a dry extract (Neiva et al ., 2014).…”

Section: Methodsmentioning

confidence: 99%

“…Toxicity tests were performed according to Rodrigues et al . (2020), with some modifications. Briefly, 24-well culture plates (Costar, Corning, NY, USA) were used with 10 active cercariae per well.…”

Section: Methodsmentioning

confidence: 99%

“…All procedures were performed according to Rodrigues et al . (2020), with some modifications. The tests were performed in triplicate (biological replicates) with three replications (technical replicates).…”

Section: Methodsmentioning

confidence: 99%

“…The treatment was considered effective when the worms did not show any movement after 2 min of observation (for analysis time). All procedures were performed according to Rodrigues et al (2020), with some modifications. The tests were performed in triplicate (biological replicates) with three replications (technical replicates).…”

Section: Toxicity Assay Against Adult Wormsmentioning

confidence: 99%

“…Worm couples that eventually died after 30 h were not considered for this analysis. The tests were performed in triplicate (biological replicates) with two repetitions (technical replicates) employing a culture protocol as described by Soares et al (2017) and Rodrigues et al (2020).…”

Section: Egg-laying Assaymentioning

confidence: 99%

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Antiparasitic effects of ethanolic extracts of Piper arboreum and Jatropha gossypiifolia leaves on cercariae and adult worms of Schistosoma mansoni

et al. 2020

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New treatment strategies for schistosomiasis should be evaluated, since resistant strains to the only available drug, Praziquantel, have already been described. Thus, we demonstrated antiparasitic effects of ethanolic extracts of Jatropha gossypiifolia and Piper arboreum on cercariae and adult worms of Schistosoma mansoni. The bioassays were performed at 0–10 000 μg mL−1 concentration for 0–72 h. Adult worms were stained with carmine to assess external and internal damage. The chemical screening was performed using high-performance liquid chromatography. P. arboreum displayed the best cercaricidal effect, with a 100% reduction in viability in just 60 min. The extract of J. gossypiifolia was more effective against adult worms, with 100% viability reduction of male and female worms after 12 and 24 h, respectively. P. arboreum and J. gossypiifolia were equally effective in inhibiting the oviposition of S. mansoni (93% reduction) and causing damage to internal and external structures in adult worms. Flavonoids were identified in both the extracts and phenolic compounds and amides only in P. arboreum. Thus, for the first time, it was proven that ethanolic extracts of P. arboreum and J. gossypiifolia leaves are biologically active against cercariae and adult worms of S. mansoni in vitro.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Toxicity Assay Against Adult Wormsmentioning

confidence: 99%

Section: Egg-laying Assaymentioning

confidence: 99%

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