The identification of metallothionein in rare minnow (Gobiocypris rarus) and its expression following heavy metal exposure

Zhang, Futie; Cao, Weiguo; Wang, Jianwei

doi:10.1016/j.etap.2014.04.021

Cited by 23 publications

(8 citation statements)

References 29 publications

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“…Similar results have been described in various fish species (Parvez et al 2003;Jebali et al 2008). However, the induction of metallothionein gene expression in cyprinid fish species following copper exposure was also detected in liver, spleen, kidney, and gills by (Wang et al 2014), and in liver and kidney by Cho et al (2008). Metallothionein induction in gills could be explained as a response to the direct contact of gill tissue with copper.…”

Section: Discussionsupporting

confidence: 70%

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Ševčíková¹,

Modrá²,

Blahová³

et al. 2016

Vet. Med.

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ABSTRACT:The aim of the present study was to investigate the impact of copper-based pesticides (at concentrations of copper of 20, 30, 40 and 70 µg/l) on one-year-old common carp (Cyprinus carpio L.) during 28 days of exposure. Abnormal behaviour was observed in fish exposed to 70 µg/l from Day 14. Histological alterations were noticed only in liver in the groups exposed to 40 and 70 µg/l. Significant changes (P ˂ 0.05) in almost all haematological indices were found, especially in the group exposed to the highest concentration of copper (70 µg/l). Biochemical analysis revealed various significant (P ˂ 0.05) differences among the tested groups. Significant differences in copper tissue concentration (P ˂ 0.05) among groups were found in liver, gills and kidney. Among antioxidative enzymes, significant changes were revealed mainly in catalase and glutathione-S-transferase activity (P ˂ 0.05). In gills, metallothionein content increased significantly (P ˂ 0.05) in the group exposed to the highest copper concentration (70 µg/l) compared with the other tested groups, including the control. A significant (P ˂ 0.05) change in total glutathione content was recorded in liver and gills, although the reduced/oxidised ratio was not affected. Oxidative damage to lipids increased significantly (P ˂ 0.05) with increasing copper concentration in liver and kidney. The results demonstrate the deleterious influence of copper on common carp even at low, environmentally relevant concentrations.

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Section: Discussionsupporting

confidence: 70%

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Ševčíková¹,

Modrá²,

Blahová³

et al. 2016

Vet. Med.

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“…Metallothionein transcript levels in livers are positively related to the average pollution index in the muscles of wild crucian carp ( Carassius auratus auratus ). Significantly positive relationships were also found between Cd dosages and MT abundance in rare minnow . Metallothionein is also a potential biomarker of acute Cd exposure in yellow catfish Pelteobagrus fulvidraco ( Siluriformes ) .…”

Section: Introductionmentioning

confidence: 98%

Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium

Liu

Yan

Wang

et al. 2016

Enviro Toxic and Chemistry

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Zebrafish embryo toxicity testing has become a popular method for detecting environmental pollutions. However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal-sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1-cell stage zebrafish embryos. The authors obtained an mt:egfp transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose- and time-dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. Environ Toxicol Chem 2016;35:2066-2073. © 2016 SETAC.

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“…conserved among vertebrates and invertebrates (Erdo gan et al, 2011;Ren et al, 2003;Wang et al, 2014). Furthermore, Cu and Fe co-exposure differentially influenced mRNA levels of MT1 and MT2, reflecting the functional differences between MT1 and MT2, as suggested by Erdo gan et al (2011).…”

Section: Discussionmentioning

confidence: 78%

Five metal elements homeostasis-related genes in Synechogobius hasta: Molecular characterization, tissue expression and transcriptional response to Cu and Fe exposure

et al. 2016

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The identification of metallothionein in rare minnow (Gobiocypris rarus) and its expression following heavy metal exposure

Cited by 23 publications

References 29 publications

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Biochemical, haematological and oxidative stress responses of common carp (Cyprinus carpio L.) after sub-chronic exposure to copper

Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium

Five metal elements homeostasis-related genes in Synechogobius hasta: Molecular characterization, tissue expression and transcriptional response to Cu and Fe exposure

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