The Identification of Key Genes and Biological Pathways in Heart Failure by Integrated Bioinformatics Analysis

Abstract

Purpose. Heart failure (HF) is a clinical syndrome caused by ventricular insufficiency. In order to further explore the biomarkers related to HF, we apply the high-throughput database. Materials and Methods. The GSE21610 was applied for the differentially expressed gene (DEG) analysis. The Database for Annotation, Visualization, and Integrated Discovery (DAVID) was performed to assess Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The Gene Set Enrichment Analysis (GSEA) was used for gene expression profile GSE21610. The Protein-Protein Interaction (PPI) network and modules were also constructed for research. These hub gene function pathways were estimated in HF progression. Result. We have identified 434 DEGs in total, including 304 downregulated DEGs and 130 upregulated DEGs. GO and KEGG illustrated that DEGs in HF were significantly enriched in G protein-coupled receptor binding, peroxisome, and cAMP signaling pathway. GSEA results showed gene set GSE21610 was gathered in lipid digestion, defense response to fungus, and intestinal lipid absorption. Finally, through analyzing the PPI network, we screened hub genes CDH1, TFRC, CCL2, BUB1B, and CD19 by the Cytoscape software. Conclusion. This study uses a series of bioinformatics technologies to obtain hug genes and key pathways related to HF. These analysis results provide us with new ideas for finding biomarkers and treatment methods for HF.

Identification and verification of feature biomarkers associated in heart failure by bioinformatics analysis

“…For example, the Vijayakrishna Kolur team concluded that HF is related to the adaptive immune system and neutrophil degranulation by analyzing GSE141910, and obtained 10 characteristic genes 7 . Yang et al 8 analyzed GSE21610 and believed that HF is related to G protein-coupled receptor binding, peroxisome and cAMP signaling pathways, and obtained 5 characteristic genes. Therefore, our work can complement theirs to gain a more comprehensive understanding of the molecular mechanisms of heart failure.…”

Identification of signature genes and functional genetic variants in heart failure by integrated bioinformatics analysis

“…Bioinformatics is a high-throughput technique, allowing high-resolution, genome-wide investigation of genetic variants, epigenetic modi cations, and associated gene expression pro les, that can screen multiple databases to identify the potential pathological biomarkers of various diseases, including cardiovascular diseases [7,8]. For example, the high-throughput RNA sequencing data has been widely employed to screen the DEGs between normal and HF samples [9,10]. And KEGG is a database resource integrated the information of genomes, biological pathways, and diseases [11,12].…”