The <i>Arabidopsis</i> Cks1At protein binds the cyclin‐dependent kinases Cdc2aAt and Cdc2bAt

Veylder, Lieven De; Segers, Gerda; Glab, Nathalie; Casteels, Peter; Montagu, Marc Van; Inzé, Dirk

doi:10.1016/s0014-5793(97)00822-3

Cited by 93 publications

(68 citation statements)

References 33 publications

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“…Protein extracts were prepared by grinding material in homogenization buffer (De Veylder et al, 1997). Protein concentrations were determined with the protein assay kit (Bio-Rad).…”

Section: Antibodies and Protein Gel Blot Analysismentioning

confidence: 99%

“…Protein concentrations were determined with the protein assay kit (Bio-Rad). CDKs were purified by affinity chromatography with either p10 CKS1At -or p9 CKS1Hs -Sepharose beads as described (De Veylder et al, 1997). Protein gel blotting was performed according to standard procedures with primary anti-CDKA;1 and anti-CDKB1;1 antibodies (Porceddu et al, 2001) diluted 1:5000 and 1:2500, respectively, and a secondary horseradish peroxidase-conjugated sheep anti-rabbit antibody (GE-Healthcare) diluted 1:5000 or with the mouse monoclonal horseradish peroxidase-conjugated anti-phosphotyrosine p-Tyr antibody (PY99; Santa Cruz Biotechnology) diluted 1:5000.…”

Section: Antibodies and Protein Gel Blot Analysismentioning

confidence: 99%

“…Proteins were detected by a chemiluminescence procedure (NEN Life Science Products). Kinase assays were performed as described previously (De Veylder et al, 1997).…”

Section: Antibodies and Protein Gel Blot Analysismentioning

confidence: 99%

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ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

Schutter¹,

Joubès²,

et al. 2007

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Upon the incidence of DNA stress, the ataxia telangiectasia-mutated (ATM) and Rad3-related (ATR) signaling kinases activate a transient cell cycle arrest that allows cells to repair DNA before proceeding into mitosis. Although the ATM-ATR pathway is highly conserved over species, the mechanisms by which plant cells stop their cell cycle in response to the loss of genome integrity are unclear. We demonstrate that the cell cycle regulatory WEE1 kinase gene of Arabidopsis thaliana is transcriptionally activated upon the cessation of DNA replication or DNA damage in an ATR-or ATM-dependent manner, respectively. In accordance with a role for WEE1 in DNA stress signaling, WEE1-deficient plants showed no obvious cell division or endoreduplication phenotype when grown under nonstress conditions but were hypersensitive to agents that impair DNA replication. Induced WEE1 expression inhibited plant growth by arresting dividing cells in the G2-phase of the cell cycle. We conclude that the plant WEE1 gene is not rate-limiting for cycle progression under normal growth conditions but is a critical target of the ATR-ATM signaling cascades that inhibit the cell cycle upon activation of the DNA integrity checkpoints, coupling mitosis to DNA repair in cells that suffer DNA damage.

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“…Protein extracts were prepared by grinding material in homogenization buffer (De Veylder et al, 1997). Protein concentrations were determined with the protein assay kit (Bio-Rad).…”

Section: Antibodies and Protein Gel Blot Analysismentioning

confidence: 99%

Section: Antibodies and Protein Gel Blot Analysismentioning

confidence: 99%

See 1 more Smart Citation

ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

Schutter¹,

Joubès²,

et al. 2007

Self Cite

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“…4). However, it is now well documented that p13 Suc1 has affinity for CDKA;1 (De Veylder et al, 1997), which has high activity at both the S-and M-phase of the cell cycle (for review, see Mironov et al, 1999). Consequently, to more precisely identify the nature of the cyclin/CDK complexes affected by Zeama;KRP;1 and Zeama;KRP;2, we tested their inhibitory effects on what are presumably S-phase (cyclin A1;3 and cyclin D5;1) and M-phase (cyclin B1;3) CDKs.…”

Section: Zeama;krps Are Ckis That Can Affect Cyclin A/cdk and Cyclin mentioning

confidence: 99%

“…Based on earlier studies that showed that maturation (mitosis)-promoting factor binds p13 Suc1 and phosphorylates histone H1, they proposed this kinase activity was related to maturation (mitosis)-promoting factor and that the inhibitor specifically affected an M-phase CDK. However, De Veylder et al (1997) subsequently showed that the Arabidopsis, fission yeast, and human Suc1/Cks1 protein can bind CDKA;1, and the associated kinase activity is high at S-, G2-, and M-phase (for review, see Mironov et al, 1999). This implies that the endosperm CKI identified by Grafi and Larkins (1995) could act at S-and/or M-phase.…”

Section: Zeama;krps Account For Only Part Of the Cdk Inhibitory Activmentioning

confidence: 99%

Cyclin-Dependent Kinase Inhibitors in Maize Endosperm and Their Potential Role in Endoreduplication

et al. 2005

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Two maize (Zea mays) cyclin-dependent kinase (CDK) inhibitors, Zeama;KRP;1 and Zeama;KRP;2, were characterized and shown to be expressed in developing endosperm. Similar to the CDK inhibitors in Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum), the maize proteins contain a carboxy-terminal region related to the inhibitory domain of the mammalian Cip/Kip inhibitors. Zeama;KRP;1 is present in the endosperm between 7 and 21 d after pollination, a period that encompasses the onset of endoreduplication, while the Zeama;KRP;2 protein declines during this time. Nevertheless, Zeama;KRP;1 accounts for only part of the CDK inhibitory activity that peaks coincident with the endoreduplication phase of endosperm development. In vitro assays showed that Zeama;KRP;1 and Zeama;KRP;2 are able to inhibit endosperm Cdc2-related CKD activity that associates with p13Suc1. They were also shown to specifically inhibit cyclin A1;3- and cyclin D5;1-associated CDK activities, but not cyclin B1;3/CDK. Overexpression of Zeama;KRP;1 in maize embryonic calli that ectopically expressed the wheat dwarf virus RepA protein, which counteracts retinoblastoma-related protein function, led to an additional round of DNA replication without nuclear division.

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The Growing Family of Plant Cyclin-Dependent Kinases with Multiple Functions in Cellular and Developmental Regulation

Dudits¹,

Cserháti²,

Miskolczi³

et al.

Cell Cycle Control and Plant Development

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The Arabidopsis Cks1At protein binds the cyclin‐dependent kinases Cdc2aAt and Cdc2bAt

Cited by 93 publications

References 33 publications

ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

ArabidopsisWEE1 Kinase Controls Cell Cycle Arrest in Response to Activation of the DNA Integrity Checkpoint

Cyclin-Dependent Kinase Inhibitors in Maize Endosperm and Their Potential Role in Endoreduplication

The Growing Family of Plant Cyclin-Dependent Kinases with Multiple Functions in Cellular and Developmental Regulation

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