The hyperhydricity ofin vitroregenerants of grass pea (Lathyrus sativus L.) is linked with an abnormal DNA content

Ochatt, Sergio; Muneaux, Etienne; Machado, Carla; Jacas, L.; Pontécaille, Catherine

doi:10.1078/0176-1617-00682

Cited by 37 publications

(28 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this respect, the transition from the dedifferentiated status to the embryogenic one is complex and includes a number of phases (dedifferentiation, cell reactivation, cell division) and a severe metabolic and developmental reprogramming (Fehér et al 2003). On the other hand, as already discussed above, endoreduplication has a strong effect on all phases of development, including amongst others on the capacity to regenerate plants from protoplasts in pea (Ochatt et al 2000a, b) or from undifferentiated tissues in M. truncatula (Elmaghrabi and Ochatt 2006;Ochatt 2008), and also on the number of tissue layers formed during organogenesis in vitro in various protein legumes (Ochatt et al 2002a(Ochatt et al , b, 2007. Other early markers of the acquisition of embryogenesis competence identified in legume cell suspension cultures include the intracellular and medium osmolarity, the cell surface and cell wall thickness ).…”

Section: Auxin In Embryo Development Seed Filling and Endoreduplicationmentioning

confidence: 97%

Agroecological impact of an in vitro biotechnology approach of embryo development and seed filling in legumes

Ochatt

2014

Agron. Sustain. Dev.

View full text Add to dashboard Cite

Ongoing global climatic changes and growing demographic pressure have increased demand for agronomic resources and affected the agroecosystem by provoking a number of abiotic stresses that, added to biotic ones, result in physiological and metabolic disorders. Such stresses ultimately impact yield when it most needs to be improved, and understanding and resolving them is a major scientific and agronomic challenge of this century. However, many species are difficult to breed for stress resistance and improved yield for a number of reasons, ranging from a long life cycle (woody species), a reduced genetic background (most self-fertile, cleistogamous legumes) or conversely extensive heterozygosity resulting from an outbreeding nature, and also due to the mainly multigenic origin of such resistances. Biotechnologybased breeding would be an efficient alternative but, for recalcitrant crops, many attempts at in vitro regeneration met with varying degrees of success and often limited to a few genotypes, hampering exploitation of biotechnology approaches. To reduce the risk of undirected somaclonal variations amongst regenerants and transformants, it is better to produce them through somatic embryogenesis that recognises a single-cell origin but whose feasibility is also limited amongst species. There is also a need to fix the resulting genome once a novelty is obtained to ensure efficient heritability of improved traits acquired, which takes several generations in conventional breeding. Acceleration of generations through flowering and fruit set in vitro has been developed in various species including legumes. Haplo-diploidisation in vitro also offers a unique alternative to conventional methods, as it yields novel genetic combinations following doubling of haplotypes and regeneration of fertile plants having gained homozygosity within a single generation. This review will examine the relationships between embryogenesis, stress and its impact on in vitro development of novel genotypes more apt for a sustainable agriculture.

show abstract

Section: Auxin In Embryo Development Seed Filling and Endoreduplicationmentioning

confidence: 97%

Agroecological impact of an in vitro biotechnology approach of embryo development and seed filling in legumes

Ochatt

2014

Agron. Sustain. Dev.

View full text Add to dashboard Cite

show abstract

“…An abnormal DNA content was systematically associated with severe hyperhydricity symptoms in grass pea ( Lathyrus sativus ), which hampered the regeneration of rooted, fertile plants [ 31 ]. They also suggested that the hyperhydric responses observed were more strongly linked to the presence of auxin in the medium and the hormonal balance between auxins and cytokinins than to the cytokinin level and auxins already have been reported to induce cytogenetic modifi cations in tissue cultures of grain legumes [ 32 ] and could be responsible for the abnormal DNA content observed in hyperhydric tissues.…”

Section: Abnormal Dna Contentmentioning

confidence: 99%

Biochemical and Physiological Aspects of Hyperhydricity in Liquid Culture System

Dewir

Indoliya

Chakrabarty

et al. 2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

View full text Add to dashboard Cite

Large-scale liquid cultures and automation have proven the potential to resolve manual handling of in vitro cultures at various stages and decreases production cost. However, hyperhydricity is a major problem during in vitro culture of many crops in liquid culture systems. The environment inside culture vessel normally used in plant micropropagation is characterized by high humidity, limited gaseous exchange between the internal atmosphere of the culture vessel and its surrounding environment, and the accumulation of ethylene, conditions that may induce physiological disorders. Hyperhydricity is a disorder of tissue-cultured plants where leaves become translucent and stems swollen, distorted and brittle. Although numerous hypotheses have been put forward to explain hyperhydricity but there is still a lack of knowledge about the nature of signals responsible for hyperhydricity and the metabolic processes which are affected by its development. The concept of stress in relation to hyperhydricity is not completely established. Therefore, it remains diffi cult to assume that hyperhydric tissues are stressed. Previous studies argued that abnormal morphology observed in hyperhydricity could be attributed to changes occurring at cellular level due to the modifi cations of membrane composition or DNA content. In order to understand stress and morphological responses in hyperhydric tissues, in the present article, we are reviewing different biochemical and physiological mechanisms of hyperhydricity in several plant species.

show abstract

“…As partes aéreas propagadas in vitro estão sob contínua condição de estresse (GASPAR et al, 2002;KEVERS et al, 2004) (PICOLI et al, 2001;GASPAR et al, 2002;ORCHATT et al, 2002). O acúmulo de ROS e a indução do estresse oxidativo foram observados na maioria dos tecidos hiperídricos descritos na literatura, entretanto, pouco é conhecido sobre a origem do estresse oxidativo induzido em tecidos hiperídricos (FERNANDEZ-GARCIA et al, 2011).…”

Section: Fatores Indutores Da Hiperidricidadeunclassified

Hiperidricidade: uma desordem metabólica

et al. 2012

View full text Add to dashboard Cite

The hyperhydricity ofin vitroregenerants of grass pea (Lathyrus sativus L.) is linked with an abnormal DNA content

Cited by 37 publications

References 17 publications

Agroecological impact of an in vitro biotechnology approach of embryo development and seed filling in legumes

Agroecological impact of an in vitro biotechnology approach of embryo development and seed filling in legumes

Biochemical and Physiological Aspects of Hyperhydricity in Liquid Culture System

Hiperidricidade: uma desordem metabólica

Contact Info

Product

Resources

About