One of the effective means of disinfecting premises is its irradiation with ultraviolet bactericidal radiation (UVBV), which is in the UV-C region (200 nm - 280 nm) and for mercury lamps has a radiation peak at a wavelength of 254 nm. However, UVBV should be used competently and with appropriate caution in terms of its intensity and safety, avoiding risks of damage to human skin and eyes. Therefore, UVBV is used indoors, mainly in the absence of a person. But when using sources of this type of optical radiation in the presence of a person, proper attention should be paid to controlling the intensity of UVBV and the time of its exposure. At the same time, it should be noted that the active processes of harmonization of the legislation of the EU and Ukraine require the modernization of the regulatory and methodological base for the measurement and hygienic assessment of ultraviolet radiation in the production environment and in the environment, significantly expanding the possibilities of using UVB sources, including in the presence of humans. Therefore, the purpose of the study is to create the basis for appropriate methodological recommendations for the use of bactericidal ultraviolet monochrome LED sources for disinfection of air and surfaces in rooms, as well as the formulation of appropriate requirements for UV radiometers, which should ensure control of the technical parameters of the mentioned ultraviolet monochrome LED sources. As a result of the research, scientific principles were proposed for the instrumental and methodical control of the safe use of bactericidal ultraviolet monochrome LED sources for the improvement of air and surfaces in premises of various purposes, taking into account the requirements of the legislation of Ukraine and the EU. The technical characteristics of the available UVBV LED sources were analyzed, based on which the medical and technical requirements for UV radiometers were formulated, which should ensure control of the intensity and actinic dose of UV radiation in accordance with the requirements of DSTU EN 62471:2017 "Safety of photobiological lamps and lamp systems (EN 62471:2008 , IDT; IES 62471:2006, MOD)". Design approaches to the formation of the spectral range of RG sensitivity by using appropriate light filters, including for the blue light range (380-480 nm), have been determined. A technique for correcting the spectral characteristic of the RG sensitivity is proposed, which contributes to the reduction of the intensity measurement error created by different LED sources.