The hydrolysis of agro-industrial residues by holocellulose-degrading enzymes

Moreira, Leonora Rios de Souza; Ferreira, Gaspar Virgilio; Santos, Sheila Sousa Thurler; Ribeiro, Ana Paula Souza; Siqueira, Félix Gonçalves de; Filho, Edivaldo Ximenes Ferreira

doi:10.1590/s1517-83822012000200010

Cited by 22 publications

(19 citation statements)

References 15 publications

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“…Devido à composição rica em açúcares fermentáveis e nutrientes dos resíduos agroindustriais, os micro-organismos têm a capacidade de utilizar esses substratos e, subsequentemente, os converterem em vários produtos de importância industrial, como enzimas, por exemplo (MOREIRA et al, 2012;PANDA et al, 2016;RAFATULLAH et al 2010). Neste sentido, novas rotas tecnológicas têm sido desenvolvidas para utilizar a biomassa oriunda de resíduos agroindustriais em substituição aos substratos convencionais e não renováveis empregados em bioprocessos (TAMANINI e HAULY, 2004).…”

Section: Introductionunclassified

Fermentação Em Estado Sólido Em Resíduos Agroindustriais Para a Produção De Enzimas: Uma Revisão Sistemática

Solidade

Souza

Sampaio

et al. 2018

JCEC

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O objetivo deste trabalho foi realizar uma revisão sistemática sobre produção de enzimas microbianas sob FES em resíduos agroindustriais, a fim de identificar as principais classes de enzimas obtidas por esse processo, os resíduos mais empregados como substratos e micro-organismos utilizados como agentes da fermentação. Um total de 37 trabalhos científicos foi selecionado e formaram um banco de dados. Aproximadamente, 49% dos trabalhos foram de nacionalidade brasileira e apontaram celulase como principal enzima produzida sob FES em resíduos agroindustriais. Fungos filamentosos, especialmente a espécie Aspergillus niger, são os mais empregados como agentes biológicos, farelo de trigo e resíduos da fruticultura foram os resíduos mais estudados e tempo ideal de fermentação foi o parâmetro mais avaliado em processos de otimização. FES é uma interessante rota tecnológica para reaproveitar resíduos da agroindústria gerando produtos de alto valor agregado.

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Section: Introductionunclassified

Fermentação Em Estado Sólido Em Resíduos Agroindustriais Para a Produção De Enzimas: Uma Revisão Sistemática

Solidade

Souza

Sampaio

et al. 2018

JCEC

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“…Thus, using lignocellulosic materials reduces its negative impact on the environment by minimizing the accumulation of waste. It also has a significant positive impact on the global economy (Alonso et al 2008;Moreira et al 2012;.…”

Section: Introductionmentioning

confidence: 99%

A Novel Approach to Delignify Lignocellulosic Materials by Using Ligninolytic Enzyme Consortium

et al. 2016

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Lignin biodegradation is an attractive approach for producing value-added products. These valuable products are produced by the processing and refining of lignocellulosic residues. A set of ligninolytic enzymes including lignin peroxidase (LiP), manganese-dependent peroxidase (MnP), and laccase (Lac) were individually produced from Ganoderma lucidum, Trametes versicolor, and Pleurotus ostreatus. Solid state fermentation under pre-optimized culture conditions with varying ratios of enzymes were used for the delignification of lignocellulosic biomass residues. The fungal enzymes were purified in four steps including ammonium sulfate precipitation, dialysis, ion exchange chromatography, and gel filtration chromatography. The purified enzymes were subsequently used in varying ratios (with each containing 200 U/mL) for the delignification of wheat straw, sugarcane bagasse, and rice straw. The consortium of enzymes caused the removal of 58.5%, 46%, and 52% of the lignin from the wheat straw, sugarcane bagasse, and rice straw, respectively, at LiP: MnP: Lac ratios of 1:2:2, 1:1:2, and 2:1:2. The best delignification was observed in wheat straw (58.5%), exposing 76.54% cellulose content. The results suggested that the ligninolytic enzymes are effective catalysts for the selective partial delignification of lignocellulosic biomass residues. After delignification these lignocellulosic residues could be utilized as costeffective substrates for the production of enzymes, biofuels, and other industrially significant products.

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“…The capacity of a particular microorganism to grow in lignocellulosic substrates is directly related to the production of a broad spectrum of enzymes that act synergistically to deconstruct the plant cell wall by depolymerization of substrates of different complexities (Andreaus et al 2008;Kumar et al 2008;Siqueira et al 2010;Moreira et al 2012). An assorted library of cellulolytic microbes should facilitate the development of optimal enzyme cocktails specific for locally available lignocellulosic biomass, such as sugarcane bagasse.…”

Section: Introductionmentioning

confidence: 99%

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

Gikonyo¹

2015

Sugarcane as Biofuel Feedstock

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Brazil is a major producer of agro-industrial residues, such as sugarcane bagasse, which could be used as raw material for microbial production of cellulases as an important strategy for the development of sustainable processes of second generation ethanol production. For this purpose, this work aimed at screening for glycosyl hydrolase activities of fungal strains isolated from the Brazilian Cerrado. Among 13 isolates, a Trichoderma harzianum strain (L04) was identified as a promising candidate for cellulase production when cultured on in natura sugarcane bagasse. Strain L04 revealed a well-balanced cellulolytic complex, presenting fast kinetic production of endoglucanases, exoglucanases and β-glucosidases, achieving 4,022, U.L -1 (72 h), 1,228 U.L -1 (120 h) and 1,968 U.L -1 (48 h) as the highest activities, respectively. About 60% glucose yields were obtained from sugarcane bagasse after 18 hours hydrolysis. This new strain represents a potential candidate for on-site enzyme production using sugarcane bagasse as carbon source.

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The hydrolysis of agro-industrial residues by holocellulose-degrading enzymes

Cited by 22 publications

References 15 publications

Fermentação Em Estado Sólido Em Resíduos Agroindustriais Para a Produção De Enzimas: Uma Revisão Sistemática

Fermentação Em Estado Sólido Em Resíduos Agroindustriais Para a Produção De Enzimas: Uma Revisão Sistemática

A Novel Approach to Delignify Lignocellulosic Materials by Using Ligninolytic Enzyme Consortium

Obtaining New Cultures Of Microorganisms That Produces Cellulases And Xylanases From The Sugarcane Bagasse

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