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“…The lack of activity of PR-A in our system is of interest in view of the reports that PR-A is a dominant negative inhibitor of the steroid-mediated induction by many other receptors, including glucocorticoid receptors (36,37). While the transfected PR-A receptors were active in CV-1 cells in the presence of 10 nM R5020, they did not reduce the fold induction by 1 M Dex-bound glucocorticoid receptors in HeLa cells.…”
Section: Discussionmentioning
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“…The lack of activity of PR-A in our system is of interest in view of the reports that PR-A is a dominant negative inhibitor of the steroid-mediated induction by many other receptors, including glucocorticoid receptors (36,37). While the transfected PR-A receptors were active in CV-1 cells in the presence of 10 nM R5020, they did not reduce the fold induction by 1 M Dex-bound glucocorticoid receptors in HeLa cells.…”
Section: Discussionmentioning
“…We chose progesterone receptors not only because of their high overall homology with glucocorticoid receptors (35) but also because the smaller A-form of human progesterone receptors (PR-A) has been found to repress the induction by several receptors including the glucocorticoid receptor under conditions where PR-A itself is either inactive (36) or active (37). Addition of up to 10 nM R5020 to HeLa cells transfected with the GREtkCAT reporter afforded no induction, consistent with the absence of endogenous progesterone receptors, while a 7-fold induction was seen with co-transfected human PR-A receptors (data not shown).…”
Section: Effect Of Transiently Transfected Receptors Requires Entire mentioning
“…In line with our findings, Spencer et al (1998) reported an increase in hippocampal MR levels after administration of the highly selective GR antagonist RU 40555. Furthermore, RU 38486 has been shown to control MR expression via human PR (McDonnell et al, 1994). Previously, Reul et al (unpublished) observed that MR upregulation was triggered by the MR antagonist spironolactone via an intrinsic mechanism, because the effect was seen in adrenalectomized rats.…”
Section: Discussionmentioning
“…The fimbrial region functions to capture and transport oocytes to the ampullary region where sperm capacitation, oocytesperm binding, fertilization and early embryo cleavage takes place. Progesterone responsive genes are more likely to be activated in the ampullary region of the oviduct than in the fimbrial region due to the change in PRAB to PRB ratio (9,10).…”
Section: Discussionmentioning