The HOP1 gene encodes a meiosis-specific component of yeast chromosomes

Hollingsworth, Nancy M.; Goetsch, Loretta; Byers, Breck

doi:10.1016/0092-8674(90)90216-2

Cited by 317 publications

(304 citation statements)

References 44 publications

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“…Defects either in the initiation or the processing of recombination intermediates have profound consequences on the output of meiosis: they can stop meiotic cell cycle progression and/or induce missegregation of the homologues leading to inviable products (Rockmill & Roeder 1988;Hollingsworth et al 1990;Roeder 1995). Sequence divergence can also have profound effects on meiosis.…”

Section: Introductionmentioning

confidence: 99%

Sensing of DNA non‐homology lowers the initiation of meiotic recomination in yeast

Rocco

Nicolas

1996

Genes to Cells

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Background: Meiotic recombination between homologous chromosomes in the yeast Saccharo-myces cerevisiae is initiated by the formation of DNA double-strand breaks (DSBs). The mechanism of DSB formation and the factors that determine their frequency and location have yet to be elucidated. Current studies of meiotic recombination are also concerned with the question of the functional relationship between DSB formation and the other meiotic processes of homology searching, pairing and synapsis of homologues.

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Section: Introductionmentioning

confidence: 99%

Sensing of DNA non‐homology lowers the initiation of meiotic recomination in yeast

Rocco

Nicolas

1996

Genes to Cells

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“…To address this issue, we examined the expression of HOP1, a gene that is not expressed in vegetative cells but is specifically induced in prophase of Meiosis I (Hollingsworth et al, 1990;Vershon et al, 1992). Using a plasmid-borne HOP1-lacZ gene fusion as a reporter (Vershon et al, 1992), we found that no activity was expressed in vegetatively growing sec14-1 cells, as expected, but that expression was induced upon transfer to sporulation medium at both permissive (25°C) and restrictive (34.5°C) temperatures (unpublished data).…”

Section: Sec14p Is Required For Meiosis and Spore Formationmentioning

confidence: 99%

Roles of Phosphoinositides and of Spo14p (phospholipase D)-generated Phosphatidic Acid during Yeast Sporulation

Rudge

Sciorra

Iwamoto

et al. 2004

MBoC

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During yeast sporulation, internal membrane synthesis ensures that each haploid nucleus is packaged into a spore. Prospore membrane formation requires Spo14p, a phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P 2 ]-stimulated phospholipase D (PLD), which hydrolyzes phosphatidylcholine (PtdCho) to phosphatidic acid (PtdOH) and choline. We found that both meiosis and spore formation also require the phosphatidylinositol (PtdIns)/PtdCho transport protein Sec14p. Specific ablation of the PtdIns transport activity of Sec14p was sufficient to impair spore formation but not meiosis. Overexpression of Pik1p, a PtdIns 4-kinase, suppressed the sec14-1 meiosis and spore formation defects; conversely, pik1-ts diploids failed to undergo meiosis and spore formation. The PtdIns(4)P 5-kinase, Mss4p, also is essential for spore formation. Use of phosphoinositide-specific GFP-PH domain reporters confirmed that PtdIns(4,5)P 2 is enriched in prospore membranes. sec14, pik1, and mss4 mutants displayed decreased Spo14p PLD activity, whereas absence of Spo14p did not affect phosphoinositide levels in vivo, suggesting that formation of PtdIns(4,5)P 2 is important for Spo14p activity. Spo14p-generated PtdOH appears to have an essential role in sporulation, because treatment of cells with 1-butanol, which supports Spo14p-catalyzed PtdCho breakdown but leads to production of Cho and Ptd-butanol, blocks spore formation at concentrations where the inert isomer, 2-butanol, has little effect. Thus, rather than a role for PtdOH in stimulating PtdIns(4,5)P 2 formation, our findings indicate that during sporulation, Spo14p-mediated PtdOH production functions downstream of Sec14p-, Pik1p-, and Mss4p-dependent PtdIns(4,5)P 2 synthesis.

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“…HOPl, which is is required for pairing of homologous chromosomes (Hollingsworth and Byers 1989;Hollingsworth et al 1990), is expressed coincidentally with a large number of other meiotic genes such as spoil (Atcheson et al 1987), REDl (Thompson and Roeder 1989), and REC114 (Pittman et al 1993), which encode products required for successful comple tion of meiosis (for review, see Mitchell 1994). DITl, which is expressed just prior to spore formation, is re quired for deposition of the outermost layer of the spore wall (Briza et al 1990b).…”

Section: Spsl Is Expressed As Meiosis Is Nearing Completionmentioning

confidence: 99%

Mutation of the SPS1-encoded protein kinase of Saccharomyces cerevisiae leads to defects in transcription and morphology during spore formation.

Friesen¹,

Lunz²,

Doyle³

et al. 1994

Genes Dev.

116

132

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During spoiulation of Saccharomyces cerevisiae, meiosis is followed by encapsulation of haploid nuclei within multilayered spore walls. Completion of the late events of the sporulation program requires the SPSl gene. This developmentally regulated gene, which is expressed as cells are nearing the end of meiosis, encodes a protein with homology to serine/threonine protein kinases. The catalytic domain of Spsl is 44% identical to the kinase domain of yeast Ste20, a protein involved in the pheromone-induced signal transduction pathway. Cells of a MATa./MATa. spsl/spsl strain arrest after meiosis and fail to activate genes that are normally expressed at a late time of sporulation. The mutant cells do not form refractile spores as assessed by phase-contrast microscopy and do not display the natural fluorescence and ether resistance that is characteristic of mature spores. Examination by electron microscopy reveals, however, that prospore-like compartments form in some of the mutant cells. These immature spores lack the cross-linked surface layer that surrounds wild-type spores and are more variable in size and number than are the spores of wild-type cells. Despite their inability to complete spore formation, spsl-arrested cells are able to resume mitotic growth on transfer to rich medium, generating haploid progeny. Our results suggest that the developmentally regulated Spsl kinase is required for normal progression of transcriptional, biochemical, and morphological events during the later portion of the sporulation program.

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The HOP1 gene encodes a meiosis-specific component of yeast chromosomes

Cited by 317 publications

References 44 publications

Sensing of DNA non‐homology lowers the initiation of meiotic recomination in yeast

Sensing of DNA non‐homology lowers the initiation of meiotic recomination in yeast

Roles of Phosphoinositides and of Spo14p (phospholipase D)-generated Phosphatidic Acid during Yeast Sporulation

Mutation of the SPS1-encoded protein kinase of Saccharomyces cerevisiae leads to defects in transcription and morphology during spore formation.

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