The Hollow Fiber Assay

Hall, Leslie-Ann M.; Krauthauser, Candice M.; Wexler, Roseanne S.; Slee, Andrew M.; Kerr, Janet S.

doi:10.1385/1-59259-323-2:545

Cited by 7 publications

(11 citation statements)

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“…This method was developed at the U.S. National Cancer Institute (NCI) [58–60] and transferred to the University of Illinois at Chicago nearly ten years ago [61]. In our hands, we have found this method to be a useful secondary assay to help determine if a given in vitro -active compound is likely to be active in a traditional xenograft assay [61].…”

Section: Biological Evaluation Of Samplesmentioning

confidence: 99%

Discovery of anticancer agents of diverse natural origin

Kinghorn

Blanco

Chai

et al. 2009

Pure and Applied Chemistry

105

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A collaborative multidisciplinary research project is described in which new natural product anticancer drug leads are obtained from a diverse group of organisms, constituted by tropical plants, aquatic cyanobacteria, and filamentous fungi. Information is provided on how these organisms are collected and processed. The types of bioassays are indicated in which crude extracts of these acquisitions are tested. Progress made in the isolation of lead bioactive secondary metabolites from three tropical plants is discussed.

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Section: Biological Evaluation Of Samplesmentioning

confidence: 99%

Discovery of anticancer agents of diverse natural origin

Kinghorn

Blanco

Chai

et al. 2009

Pure and Applied Chemistry

105

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“…Hollow fiber chambers (HFCs), which are opaque and large volume (hundreds of mL) perfusion-based cell culture platforms, are commonly used in pharmaceutical studies and a MTS assay is commonly used to evaluate cell proliferation in a HFCs. 30 MTS assay changes its color form light orange to purple with viable cells, with the purple color intensity being proportional to the amount of viable cells. 31 As shown in Figure 4A, even though the 3D printed devices were semi-transparent, a layer of dark purple could be observed with MTS assay on the inside of the left device, which was fiber coated on the inside.…”

Section: Resultsmentioning

confidence: 99%

“…For fiber size determination, at least 50 fibers were measured on each SEM image using ImageJ. The pore size of the fibrous scaffold was determined by the area measurement tool 29, 30 in ImageJ and at least 20 pores were measured on each SEM image.…”

Section: Methodsmentioning

confidence: 99%

Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices

Chen

Mehl

Sell

et al. 2016

Analyst

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Organs-on-a-chip has emerged as a powerful tool for pharmacological and physiological studies. A key part in the construction of such a model is the ability to pattern or culture cells in a biomimetic fashion. Most of the reported cells-on-a-chip models integrate cells on a flat surface, which does not accurately represent the extracellular matrix that they experience in vivo. Electrospinning, a technique used to generate sub-micron diameter polymer fibers, has been used as an in vitro cell culture substrate and for tissue engineering applications. Electrospinning of fibers directly into a fully sealed fluidic channel using a conventional setup has not been possible due to issues of confining the fibers into a discrete network. In this work, a dynamic focusing method was developed, with this approach enabling direct deposition of electrospun fibers into a fully sealed fluidic channel, to act as a matrix for cell culture and subsequent studies under continuous flowing conditions. Scanning electron microscopy of electrospun polycaprolactone fibers shows that this method enables the formation of fibrous layers on the inner wall of a 3D-printed fluidic device (mean fiber size = 1.6 ± 0.6 μm and average pore size = 113 ± 19 μm2). Cells were able to be cultured in this 3D scaffold without the addition of adhesion proteins. Media was pumped through the channel at high flow rates (up to 400 μL/min) during a dynamic cell culture process and both the fibers and the cells were found to be strongly adherent. A PDMS fluidic device was also prepared (from a 3D printed mold) and coated with polycaprolactone fibers. The PDMS device enables optical detection and confocal imaging of cultured cells on the fibers. Finally, macrophages were cultured in the devices to study how the fibrous scaffold can affect cell behavior. It was found that under lipopolysaccharide stimulation, macrophages cultured on PCL fibers inside of a channel secreted significantly more cytokines than those cultured on a thin layer of PCL in a channel or directly on the inner wall of a channel. Overall, this study represents a new approach and technique for in vitro cell studies, where electrospinning can be used to easily and quickly create 3D scaffolds that can improve the culture conditions in microfluidic devices.

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“…The hollow fibers that carry the tumor cells are composed of modified polyvinylidene fluoride (PVDF) [20]. PVDF fibers attract only minimal numbers of reactive host cells, which loosely attach to the fiber exterior and do not compromise the growth of tumor cells in the interior.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the therapeutic efficacy of tripeptide tyroserleutide (YSL) for human hepatocarcinoma by in vivo hollow fiber assay

Liu

Lü

et al. 2008

Invest New Drugs

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Tyroserleutide (YSL), extracted from spleen of pigs, is a tripeptide that has shown therapeutic efficacy in an experimental BEL-7402 human hepatocarcinoma model. The hollow fiber assay (HFA) is a solid tumor model for large-scale screening of potential anticancer compounds that minimizes expenditures of materials, time, and money. Tumor cells are cultivated within biocompatible, semipermeable hollow fibers, which are implanted in immunosuppressed mice. In this study, the HFA was used to investigate the therapeutic efficacy of YSL for human hepatocarcinoma. In vitro effects of YSL on human hepatocarcinoma cell lines BEL-7402, SMMC-7721, Hep3B, HepG2, and SK-HEP-1 were assayed by the MTS method. In vivo effects of YSL on the five human hepatocarcinoma cell lines were assayed by HFA. Mice implanted with tumor cells in hollow fibers were treated with YSL, and the effects of YSL on tumor cell populations were assessed by MTT assay. YSL significantly inhibited the proliferation of the five human hepatocarcinoma, both in vitro and in vivo (P < 0.05). The HFA is a rapid, accurate, and economical method for evaluating the inhibitory effects of drugs on different tumor cells in vivo. These results support the clinical application of YSL for treatment of human hepatocarcinoma.

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The Hollow Fiber Assay

Cited by 7 publications

References 0 publications

Discovery of anticancer agents of diverse natural origin

Discovery of anticancer agents of diverse natural origin

Use of electrospinning and dynamic air focusing to create three-dimensional cell culture scaffolds in microfluidic devices

Evaluation of the therapeutic efficacy of tripeptide tyroserleutide (YSL) for human hepatocarcinoma by in vivo hollow fiber assay

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