The HMG domain of lymphoid enhancer factor 1 bends DNA and facilitates assembly of functional nucleoprotein structures

Giese, Klaus; Cox, Jeffery S.; Grosschedl, Rudolf

doi:10.1016/0092-8674(92)90129-z

Cited by 659 publications

(537 citation statements)

References 55 publications

Supporting

Mentioning

516

Contrasting

Unclassified

Order By: Relevance

“…The DNA binding domain of SRY shares homology with a number of known or suspected transcription factors of the HMG box class [2]. Experiments in vitro suggest that SRY binds linear DNA in a sequence-specific manner [3,4] and causes a bend [5,6]. Mutations in the HMG domain of SRY encoded by XY gonadal dysgenesis patients affect the DNA binding or DNA bending activity of SRY in vitro which is consistent with these activities being necessary for testis development [7].…”

Section: Introductionmentioning

confidence: 59%

“…In the presence of CaM, peptides from two regions of the SRY HMG domain showed an interaction: residues 57-80 (lane 1) and a second, weakly binding region, 131-144, which gave multiple bands (lane 9). No CaM binding was detectable with the other six SRY peptides (lanes [3][4][5][6][7][8]. Binding affinity of SRY peptides 57-80 (QDRVKRPM-NAFIVWSRDQRRKMAL) to CaM was similar to that for the whole HMG box; binding of SRY peptides 131-144 (PRRKAKMLPKNCSL) was about five-fold weaker.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The HMG box of SRY is a calmodulin binding domain

Harley

Lovell-Badge²,

Goodfellow

et al. 1996

FEBS Letters

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%

The HMG box of SRY is a calmodulin binding domain

Harley

Lovell-Badge²,

Goodfellow

et al. 1996

FEBS Letters

View full text Add to dashboard Cite

“…RUNX1 binding to DNA is through its central runt domain, and activation of the class I promoter depends on both its DNA binding and activation domains. LEF1 is an HMG protein whose binding to DNA causes bending of the backbone (34,62,84,85). Neither RUNX1 nor LEF1 alone significantly affected MHC class I expression in the nonlymphocyte HeLa epithelial cell line, which does not express either of these factors (data not shown).…”

Section: Discussionmentioning

confidence: 99%

A T Lymphocyte-Specific Transcription Complex Containing RUNX1 Activates MHC Class I Expression

Howcroft

Weissman

Gégonne

et al. 2005

The Journal of Immunology

View full text Add to dashboard Cite

MHC class I expression is subject to both tissue-specific and hormonal regulatory mechanisms. Consequently, levels of expression vary widely among tissues, with the highest levels of class I occurring in the lymphoid compartment, in T cells and B cells. Although the high class I expression in B cells is known to involve the B cell enhanceosome, the molecular basis for high constitutive class I expression in T cells has not been explored. T cell-specific genes, such as TCR genes, are regulated by a T cell enhanceosome consisting of RUNX1, CBFβ, LEF1, and Aly. In this report, we demonstrate that MHC class I gene expression is enhanced by the T cell enhanceosome and results from a direct interaction of the RUNX1-containing complex with the class I gene in vivo. T cell enhanceosome activation of class I transcription is synergistic with CIITA-mediated activation and targets response elements distinct from those targeted by CIITA. These findings provide a molecular basis for the high levels of MHC class I in T cells.

show abstract

“…The spatio-temporal expression of genes is controlled by DNA elements called enhancers (Banerji et al 1981) or cis-regulatory modules (CRMs) (Kirchhamer et al 1996). These elements act as docking platforms for transcription factors (TFs), and the combined regulatory cues of all bound TFs results in the activation (or repression) of gene expression (e.g., Stanojevic et al 1991;Giese et al 1992).…”

mentioning

confidence: 99%

Uncovering cis-regulatory sequence requirements for context-specific transcription factor binding

et al. 2012

View full text Add to dashboard Cite

The regulation of gene expression is mediated at the transcriptional level by enhancer regions that are bound by sequence-specific transcription factors (TFs). Recent studies have shown that the in vivo binding sites of single TFs differ between developmental or cellular contexts. How this context-specific binding is encoded in the cis-regulatory DNA sequence has, however, remained unclear. We computationally dissect context-specific TF binding sites in Drosophila, Caenorhabditis elegans, mouse, and human and find distinct combinations of sequence motifs for partner factors, which are predictive and reveal specific motif requirements of individual binding sites. We predict that TF binding in the early Drosophila embryo depends on motifs for the early zygotic TFs Vielfaltig (also known as Zelda) and Tramtrack. We validate experimentally that the activity of Twist-bound enhancers and Twist binding itself depend on Vielfaltig motifs, suggesting that Vielfaltig is more generally important for early transcription. Our finding that the motif content can predict contextspecific binding and that the predictions work across different Drosophila species suggests that characteristic motif combinations are shared between sites, revealing context-specific motif codes (cis-regulatory signatures), which appear to be conserved during evolution. Taken together, this study establishes a novel approach to derive predictive cis-regulatory motif requirements for individual TF binding sites and enhancers. Importantly, the method is generally applicable across different cell types and organisms to elucidate cis-regulatory sequence determinants and the corresponding trans-acting factors from the increasing number of tissue-and cell-type-specific TF binding studies.

show abstract

The HMG domain of lymphoid enhancer factor 1 bends DNA and facilitates assembly of functional nucleoprotein structures

Cited by 659 publications

References 55 publications

The HMG box of SRY is a calmodulin binding domain

The HMG box of SRY is a calmodulin binding domain

A T Lymphocyte-Specific Transcription Complex Containing RUNX1 Activates MHC Class I Expression

Uncovering cis-regulatory sequence requirements for context-specific transcription factor binding

Contact Info

Product

Resources

About