The histone variant macroH2A1.1 regulates RNA Polymerase II paused genes within defined chromatin interaction landscapes

Recoules, Ludmila; Heurteau, Alexandre; Raynal, Flavien; Moutahir, Fatima; Bejjani, Fabienne; Jariel-Encontre, Isabelle; Cuvier, Olivier; Lavigne, Anne-Claire; Bystricky, Kerstin

doi:10.1101/2020.01.29.924704

Cited by 1 publication

(1 citation statement)

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“…Please find a description of all used antibodies in Table A2 in Appendix A. The macroH2A1.1 antibody has been generated in the laboratory of Prof. Kerstin Bystricky and is characterized in [26] For immunofluorescence, 20,000 C2C12 cells were seeded in 6 well-plates and cover slips. On day 4 of differentiation, C2C12 cells were fixed in 4% paraformaldehyde for 10 min at room temperature.…”

Section: Antibodies and Immunofluorescencementioning

confidence: 99%

The Histone Variant MacroH2A1 Regulates Key Genes for Myogenic Cell Fusion in a Splice-Isoform Dependent Manner

Hurtado-Bagès

Marjanović

Valero

et al. 2020

Cells

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MacroH2A histone variants have functions in differentiation, somatic cell reprogramming and cancer. However, at present, it is not clear how macroH2As affect gene regulation to exert these functions. We have parted from the initial observation that loss of total macroH2A1 led to a change in the morphology of murine myotubes differentiated ex vivo. The fusion of myoblasts to myotubes is a key process in embryonic myogenesis and highly relevant for muscle regeneration after acute or chronic injury. We have focused on this physiological process, to investigate the functions of the two splice isoforms of macroH2A1. Individual perturbation of the two isoforms in myotubes forming in vitro from myogenic C2C12 cells showed an opposing phenotype, with macroH2A1.1 enhancing, and macroH2A1.2 reducing, fusion. Differential regulation of a subset of fusion-related genes encoding components of the extracellular matrix and cell surface receptors for adhesion correlated with these phenotypes. We describe, for the first time, splice isoform-specific phenotypes for the histone variant macroH2A1 in a physiologic process and provide evidence for a novel underlying molecular mechanism of gene regulation.

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Section: Antibodies and Immunofluorescencementioning

confidence: 99%