The histodifferentiation events involved during the acquisition and development of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.)

Silva, Rafael de Carvalho; Luis, Zanderluce Gomes; Scherwinski‐Pereira, Jonny Everson

doi:10.1007/s10725-013-9837-0

Cited by 29 publications

(17 citation statements)

References 33 publications

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“…For the biochemical characterization of SE, the following were extracted and quantified: total soluble sugars, starch, free amino acids and total proteins from cultures at different stages of SE, according to Silva et al (2014): ZE (initial explant); primary calluses (14, 21, 30, 45 and 60 days), embryogenic calluses (90 days), calluses with pro-embryos (150 days), globular embryos (210 days), differentiated somatic embryos (300 days), and regenerated plants (360 days).…”

Section: Methodsmentioning

confidence: 99%

“…In this species, micropropagation via somatic embryogenesis (SE) is one of the only alternatives for plant clonal propagation (Scherwinski-Pereira et al 2010;Soh et al 2011;Silva et al 2014). Worldwide, millions of plants from different plant species are produced annually through SE (Ducos et al 2007;Steinmacher et al 2011;Sané et al In memoriam of Lourdes Isabel Velho do Amaral. 2012).…”

Section: Introductionmentioning

confidence: 99%

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Comparative biochemical profiling during the stages of acquisition and development of somatic embryogenesis in African oil palm (Elaeis guineensis Jacq.)

et al. 2014

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In this study, the different stages of somatic embryogenesis (SE) of the African oil palm (Elaeis guineensis Jacq.) were characterized biochemically. The total soluble sugars, starch, total free amino acids, and total proteins were extracted, identified and quantified at various stages of embryogenesis: zygotic embryos (initial explants), primary calluses, embryogenic calluses, calluses with pro-embryos, globular embryos, differentiated somatic embryos, and regenerated plants. It was found that at the onset of induction of SE, the level of soluble sugars in the tissues of the explants fell by half. During this period, the total soluble sugars present in the cultures consisted basically of glucose and fructose. In the process of regeneration and maturation, the concentrations of soluble sugars gradually increased, reaching the highest values in the last two stages of development. At this stage, the disaccharide sucrose accounts for more than 80 % of the composition of total soluble sugars in the explants. Compared to starch, we found that the concentrations thereof in developing tissues are inversely proportional to that of soluble sugars virtually throughout embryogenic development. As for free amino acids, we found that after 30 days of induction until formation of the embryogenic calluses, there is an accentuated synthesis of total free amino acids in the explant tissue. In this stage, there was a significant increase in the levels of alanine and serine in the tissues. However, after the formation of the embryogenic calluses, the levels of total free amino acids present in the cultures become stabile and remain constant until the end of cultivation. Similar results were found for total protein, which also showed a significant increase at the onset of induction, undergoing slight changes during the remainder of the cultivation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative biochemical profiling during the stages of acquisition and development of somatic embryogenesis in African oil palm (Elaeis guineensis Jacq.)

et al. 2014

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“…Asynchronous development of somatic embryos is commonly reported in the literature. Silva et al (2014) and Steinmacher et al (2007) observed distinct morphological responses, including the non-synchronous development of the somatic embryos in Elaeis guinensis and in Bactris gasipaes, respectively.…”

Section: Growth Regulators (M)mentioning

confidence: 98%

Induction of somatic embryogenesis and biochemical characterization of Cordyline australis (G. Forst.) Endl. ‘Red Star’ callus

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et al. 2015

Scientia Horticulturae

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“…According to Balzon et al (2013), the intense cell division can only be detected in the internal part of the calli and presented as a cell development process. Also, earlier reports have shown that small and isodiametric cells exhibited dense cytoplasm and starch accumulation indicating that cell development was in progress towards embryogenesis (Padua et al, 2013;de Carvalho Silva et al, 2014;Lim et al, 2016b). On the other hand, starch grain accumulation was also observed in sites of less dense cytoplasm especially for bacterised calli treated with Z78 pellet cells (T4) and bacterised embryogenic calli treated with Z78 sonicated cells (T3).…”

Section: Morpho-histological Biomass Increment and Viable Cell Countmentioning

confidence: 82%

BIOCHEMICAL PROFILE OF BACTERISED CALLI AND EMBRYOGENIC CALLI OF OIL PALM (Elaeis guineensis Jacq.)

Lim¹

2018

JOPR

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The histodifferentiation events involved during the acquisition and development of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.)

Cited by 29 publications

References 33 publications

Comparative biochemical profiling during the stages of acquisition and development of somatic embryogenesis in African oil palm (Elaeis guineensis Jacq.)

Comparative biochemical profiling during the stages of acquisition and development of somatic embryogenesis in African oil palm (Elaeis guineensis Jacq.)

Induction of somatic embryogenesis and biochemical characterization of Cordyline australis (G. Forst.) Endl. ‘Red Star’ callus

BIOCHEMICAL PROFILE OF BACTERISED CALLI AND EMBRYOGENIC CALLI OF OIL PALM (Elaeis guineensis Jacq.)

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