volume 66, issue 6, P3624-3632 1992
DOI: 10.1128/jvi.66.6.3624-3632.1992
View full text
|
|
Share

Abstract: The herpes simplex virus 1 Us11 gene encodes a site-and conformation-specific RNA binding regulatory protein. We fused the coding sequence of this protein with that of 1-galactosidase, expressed the chimeric gene in Escherichia coli, and purified a fusion protein which binds RNA in the same way as the infected cell protein. The fusion protein was used to generate anti-Us11 monoclonal antibody. Studies with this antibody showed that Us11 protein is a viral structural protein estimated to be present in 600 to 1,…

Expand abstract