The green microalga Tetraselmis suecica reduces oxidative stress and induces repairing mechanisms in human cells

Sansone, Clementina; Galasso, Christian; Orefice, Ida; Nuzzo, Genoveffa; Luongo, Elvira; Cutignano, Adele; Romano, Giovanna; Brunet, Christophe; Fontana, Angelo; Esposito, Francesco; Ianora, Adrianna

doi:10.1038/srep41215

Cited by 103 publications

(70 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, previous studies reported that the antioxidant effects in cultured human fibroblasts, irradiated with UV-A light (20 J/cm 2 ), were enhanced when a combination of antioxidant compounds (with β-carotene, as the main component, α-tocopherol and ascorbic acid) was used as a treatment [ 50 ]. In addition, a mixture of β-carotene and vitamins E and C showed a stronger synergistic scavenging effect of the reactive nitrogen species than the single antioxidants [ 51 ].Recent investigations demonstrated that the green microalga Tetraselmis suecica is characterized by a natural pool of bioactive pigments (including neoxanthin, violaxanthin, antheraxanthin, lutein, α- and β-carotene) working synergistically against oxidative damage [ 52 ]. In particular, this pool of bioactive pigments showed scavenging activity (with DPPH assay) and repairing activity when the T. suecica crude extract was used as recovery treatment in human fibroblasts after pre-injury with H 2 O 2 (30 mM for 1 h).…”

Section: Biological Functions Of Carotenoidsmentioning

confidence: 99%

See 1 more Smart Citation

Carotenoids from Marine Organisms: Biological Functions and Industrial Applications

2017

Self Cite

View full text Add to dashboard Cite

As is the case for terrestrial organisms, carotenoids represent the most common group of pigments in marine environments. They are generally biosynthesized by all autotrophic marine organisms, such as bacteria and archaea, algae and fungi. Some heterotrophic organisms also contain carotenoids probably accumulated from food or partly modified through metabolic reactions. These natural pigments are divided into two chemical classes: carotenes (such as lycopene and α- and β-carotene) that are composed of hydrogen and carbon; xanthophylls (such as astaxanthin, fucoxanthin and lutein), which are constituted by hydrogen, carbon and oxygen. Carotenoids, as antioxidant compounds, assume a key role in the protection of cells. In fact, quenching of singlet oxygen, light capture and photosynthesis protection are the most relevant biological functions of carotenoids. The present review aims at describing (i) the biological functions of carotenoids and their benefits for human health, (ii) the most common carotenoids from marine organisms and (iii) carotenoids having large success in pharmaceutical, nutraceutical and cosmeceutical industries, highlighting the scientific progress in marine species cultivation for natural pigments production.

show abstract

Section: Biological Functions Of Carotenoidsmentioning

confidence: 99%

“…T. suecica is rich in tocopherol, carotenoids and chlorophyll and has been suggested as a food supplement in human and animal diets. The total carotenoids content of T. suecica exhibited antioxidant and repairing activity in human cell lines [ 52 ].…”

Section: Sources Of Carotenoids From Marine Organismsmentioning

confidence: 99%

Carotenoids from Marine Organisms: Biological Functions and Industrial Applications

2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Rasmussen and Morrissey (2007) presented an overview on the food ingredients produced by marine organisms, highlighting microalgae and/or their bioactive ingredients. In addition, Sansone et al (2017) recently presented that an extract obtained from the green microalga Tetraselmis suecica had a strong antioxidant and repairing activity in a human cancer cell line. On the other hand, bioactive molecules for cosmetic products are characterized by their capacity to stimulate beneficial properties or to interfere with pathways leading to skin damage (Krutmann, 2001;Yarosh et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Letsiou¹,

Kalliampakou

Gardikis³

et al. 2017

Front. Mar. Sci.

View full text Add to dashboard Cite

Nowadays, there is huge interest in natural products obtained from marine organisms that can promote a state of health and well-being for humans. Microalgae represent a primary source of bioactive compounds that could be used as functional ingredients in cosmetic formulations. The aim of the present study is to evaluate, for the first time, the effects of Nannochloropsis gaditana extract against oxidative stress in human primary fibroblasts so as to investigate the potential applications of it in cosmetics. To gain an insight into the molecular mechanisms of N. gaditana bioactivity, we developed a new RT-qPCR platform for studying transcript accumulation for an array of selected genes (up to 100) involved in many skin-related processes including anti-aging, hydration, oxidative stress response, and DNA damage. For the oxidative stress evaluation, H 2 O 2 was used as a stressor. The study of the transcript accumulation of genes revealed that N. gaditana extract exhibits skin protection properties by mediating oxidative responses and apoptosis (including SOD1, GPX1, BID), positively regulates genes involves in skin texture and hydration (including AQP3, Col6A1, FBN1) and modulates the expression of genes involved in skin irritation, DNA damage and aging (including IL1R, PCNA, FOXO3). These findings indicate that the specific N. gaditana extract possesses significant in vitro skin protection activity against induced oxidative stress, and provide new insights into the beneficial role of microalgae bioactive compounds in cosmetic formulations protecting skin from oxidative stress.

show abstract

“…Extracts for pigment analysis and biological assays were obtained from freeze-dried young and old leaves of H. stipulacea, according to Sansone et al (Sansone et al 2017) at room temperature, under dark and N 2 atmosphere to avoid oxidation during procedure. One g of leaves was placed in 1 ml of ethanol/water mixture (3/1; v/v) for 30 min in dark condition and mechanically disrupted with a pestle.…”

Section: Preparation Of Ethanol/water Extracts From Halophila Stipulamentioning

confidence: 99%

Testing the Halophila Stipulacea Environmental Threat as A Potential Antioxidant Agent

Sansone

Galasso

Martire

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Marine biotechnology is promising to provide new solutions to society and humans, such as for health protection or natural treatments against diseases. In this framework, different strategies are explored to select marine organisms to initiate the pipeline, hopefully ended by the product development. Organisms with fast growth rates and great biological adaptive capacity are of biotechnological interests, since competitiveness might rely on increased physiological or biochemical processes’ capability seeking to protect, defend on repair intracellular damages. This study focussed on the fast-growing and invasive seagrass Halophila stipulacea, a non-indigenous species widespread in the Mediterranean Sea to investigate its antioxidant activity, both in scavenging reactive oxygen species and in repairing damages from oxidative stress on human fibroblast cells. Results indicated that the ethanol/water extracts of the leaves of H. stipulacea were able to scavenge reactive oxygen species and in repair damages from oxidative stress. Moreover, greater bioactivities were observed in old leaves compared to young ones, paralleled with a higher content in carotenoids and carbohydrate contribution to organic matter content. Our results suggest a potential of the (old) leaves of H. stipulacea as skin-protective and/or oxidative stress damage repair agents. This study paves the way to transmute the invasive H. stipulacea environmental threat in goods for human health.

show abstract

The green microalga Tetraselmis suecica reduces oxidative stress and induces repairing mechanisms in human cells

Cited by 103 publications

References 30 publications

Carotenoids from Marine Organisms: Biological Functions and Industrial Applications

Carotenoids from Marine Organisms: Biological Functions and Industrial Applications

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Testing the Halophila Stipulacea Environmental Threat as A Potential Antioxidant Agent

Contact Info

Product

Resources

About