The glycerol channel Fps1p mediates the uptake of arsenite and antimonite in <i>Saccharomyces cerevisiae</i>

Wysocki, Robert; Chéry, Cyrille C.; Wawrzycka, Donata; Hulle, Marijn Van; Cornelis, Rita; Thevelein, Johan M.; Tamás, Markus J.

doi:10.1046/j.1365-2958.2001.02485.x

Cited by 306 publications

(288 citation statements)

References 58 publications

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“…The competition of arsenite uptake by Sb and glycerol in C. demersum (Fig. 6) may indicate that arsenite is transported across the plasma membrane via major intrinsic proteins/aquaporins as found in Saccharomyces cerevisiae and rice (Wysocki et al 2001;Meharg and Jardine 2003). Different from the study by Zheng et al (2003) on C. demersum, no matter what arsenic species are initially supplied to the plants, most of the As (>86 %) in plant shoots was in the form of arsenite rather than arsenate (Fig.…”

Section: Discussionmentioning

confidence: 92%

Arsenic accumulation and speciation in the submerged macrophyte Ceratophyllum demersum L.

Xue

Yan

Sun

et al. 2012

Environ Sci Pollut Res

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Introduction Ceratophyllum demersum L. is a widespread submerged macrophyte in aquatic environments.Methods Simulation experiments were conducted in the laboratory to investigate arsenic (As) accumulation, speciation, and efflux of C. demersum exposed to arsenate and arsenite solutions. Results Plant shoots showed a significant accumulation of As with a maximum of 862 and 963 μg As g −1 dry weight after 4 days of exposure to 10 μM arsenate and arsenite, respectively. Regardless of whether arsenate or arsenite was supplied to the plants, arsenite was the predominant species in plant shoots. Furthermore, a dramatically higher influx rate of arsenate compared with arsenite was observed in C. demersum exposed to As solutions without the addition of phosphate (P). Arsenate uptake was considerably inhibited by P in this study, suggesting that arsenate is taken up by C. demersum via the phosphate transporters. However, arsenite uptake was unaffected by P and markedly reduced in the presence of glycerol and antimonite (Sb), indicating arsenite shares the aquaporin transport pathway. In addition, C. demersum rapidly reduces arsenate to arsenite in the shoot of the plant and extrudes most of them (>60 %) to the external solutions. The efflux of arsenite was much higher than that of arsenate; the former is supposed to be both active and passive processes, and the latter through passive leakage. Conclusion C. demersum is a strong As accumulator and an interesting model plant to study As uptake and metabolism due to the lack of a root-to-shoot translocation barrier.

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Section: Discussionmentioning

confidence: 92%

Arsenic accumulation and speciation in the submerged macrophyte Ceratophyllum demersum L.

Xue

Yan

Sun

et al. 2012

Environ Sci Pollut Res

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“…In Saccharomyces cerevisiae, arsenate gets into the cell by phosphate transporters due to the chemical similarity between As (V) and Pi (33), and is reduced to arsenite by an arsenate reductase (34). However, arsenite is taken up via aquaglyceroporins (35) or hexose permeases (36). Finally, arsenite was sequestered in the vacuole via the MRP1 homologue Ycf1p in its glutathionated form (37).…”

Section: Arsenic Uptake Transport and Detoxification In P Vittatamentioning

confidence: 99%

The Arsenic Hyperaccumulator FernPteris vittataL.

Qingen

Yan

Liao

et al. 2009

Environ. Sci. Technol.

131

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Arsenic (As) contaminated soils and waters are becoming major global environmental and human health risks. The identification of natural hyperaccumulators of As opens the door for phytoremediation of the arsenic contaminant. Pteris vittata is the first identified naturally evolving As hyperaccumulator. More than a decade after its discovery, we have made great progress in understanding the uptake, transport, and detoxification of As in the fern. The molecular mechanisms controlling As accumulation in P. vittata are now beginning to be recognized. In this review, we will try to summarize what we have learned about this As accumulator, with particular emphasis on the current knowledge of the physiological and molecular mechanisms of arsenic phytoremediation. We also discuss the potential strategies to further enhance phytoextraction abilities of P. vittata.

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“…To examine these alternatives, the transcript levels of ACR3, and FPS1, which are involved in As(III) export and uptake in S. cerevisiae, respectively [5,8], were determined (Fig. 4).…”

Section: The Decrease In Arsenic Accumulation Correlates Withmentioning

confidence: 99%

“…Arsenite (As(III)), which exists as the hydrated form of arsenic trioxide (As(OH) 3 ) in neutral solution [4], is taken up via aquaglyceroporins [5] or hexose permeases [6]. Arsenite is sequestered in the vacuole via the MRP1 homologue Ycf1p in its glutathionated form [7] or is exported via a proton motive force-coupled arsenite efflux permease [8].…”

Section: Introductionmentioning

confidence: 99%

The tobacco geneNtcyc07confers arsenite tolerance inSaccharomyces cerevisiaeby reducing the steady state levels of intracellular arsenic

et al. 2008

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We cloned a plant gene, Ntcyc07, conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3, but repressed that of As(III) uptake channel FPS1. Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3, but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(III) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1.

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The glycerol channel Fps1p mediates the uptake of arsenite and antimonite in Saccharomyces cerevisiae

Cited by 306 publications

References 58 publications

Arsenic accumulation and speciation in the submerged macrophyte Ceratophyllum demersum L.

Arsenic accumulation and speciation in the submerged macrophyte Ceratophyllum demersum L.

The Arsenic Hyperaccumulator FernPteris vittataL.

The tobacco geneNtcyc07confers arsenite tolerance inSaccharomyces cerevisiaeby reducing the steady state levels of intracellular arsenic

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