The genotoxic effect of nicotine on chromosomes of human fetal cells: The first report described as an important study

Demırhan, Osman; Demir, Cansun; Tunç, Erdal; Sütcü, Erdinç; Sadıkoğlu, Nurefşan; Özcan, Beyza

doi:10.3109/08958378.2011.617398

Cited by 25 publications

(22 citation statements)

References 39 publications

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“…Thousands of compounds are present in CS, including a large number of reactive oxygen species that can cause DNA damage and lead to the activation of poly (ADP-ribose) polymerase (PARP) enzyme [36]. Other components of CS, such as nicotine and acrolein, have also been shown to exert direct genotoxic effects [37,38]. …”

Section: Discussionmentioning

confidence: 99%

The standardized herbal formula, PM014, ameliorated cigarette smoke-induced lung inflammation in a murine model of chronic obstructive pulmonary disease

Jung

Haam

Park

et al. 2013

BMC Complement Altern Med

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BackgroundIn this study, we evaluated the anti-inflammatory effect of PM014 on cigarette smoke induced lung disease in the murine animal model of chronic obstructive pulmonary disease (COPD).MethodsMice were exposed to cigarette smoke (CS) for 2 weeks to induce COPD-like lung inflammation. Two hours prior to cigarette smoke exposure, the treatment group was administered PM014 via an oral injection. To investigate the effects of PM014, we assessed PM014 functions in vivo, including immune cell infiltration, cytokine profiles in bronchoalveolar lavage (BAL) fluid and histopathological changes in the lung. The efficacy of PM014 was compared with that of the recently developed anti-COPD drug, roflumilast.ResultsPM014 substantially inhibited immune cell infiltration (neutrophils, macrophages, and lymphocytes) into the airway. In addition, IL-6, TNF-α and MCP-1 were decreased in the BAL fluid of PM014-treated mice compared to cigarette smoke stimulated mice. These changes were more prominent than roflumilast treated mice. The expression of PAS-positive cells in the bronchial layer was also significantly reduced in both PM014 and roflumilast treated mice.ConclusionsThese data suggest that PM014 exerts strong therapeutic effects against CS induced, COPD-like lung inflammation. Therefore, this herbal medicine may represent a novel therapeutic agent for lung inflammation in general, as well as a specific agent for COPD treatment.

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Section: Discussionmentioning

confidence: 99%

The standardized herbal formula, PM014, ameliorated cigarette smoke-induced lung inflammation in a murine model of chronic obstructive pulmonary disease

Jung

Haam

Park

et al. 2013

BMC Complement Altern Med

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“…Nicotine addiction may cause deleterious effects in women's brains by inhibiting estrogen signaling, which in turn may make the brain more susceptible to ischemia (42). There are many reports of the deleterious effect of nicotine on bones in both animal models and humans (43) and of the adverse effect of nicotine on chromosomes ("genotoxic effect") of fetal cells (44).…”

Section: Health Effects Of Nicotinementioning

confidence: 99%

Electronic Cigarettes. A Position Statement of the Forum of International Respiratory Societies

Schraufnagel

Blasi

Drummond

et al. 2014

Am J Respir Crit Care Med

159

136

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The health and safety claims regarding electronic nicotine delivery devices should be subject to evidentiary review. The potential benefits of electronic cigarettes to an individual smoker should be weighed against potential harm to the population of increased social acceptability of smoking and use of nicotine, the latter of which has addictive power and untoward effects. As a precaution, electronic nicotine delivery devices should be restricted or banned until more information about their safety is available. If they are allowed, they should be closely regulated as medicines or tobacco products.

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“…Furthermore, in vitro studies confirmed that amniocytes obtained from pregnant women and cultured in medium containing nicotine showed high frequencies of structural and numerical chromosomal aberrations, involving chromosomes 21, 22, 8, 15, and 20 [10]. …”

Section: Introductionmentioning

confidence: 99%

Smoking-induced chromosomal segregation anomalies identified by FISH analysis of sperm

et al. 2014

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BackgroundNumerical chromosome aberrations in gametes are directly related to infertility and aneuploid embryos. Previous studies have shown that toxic substances from cigarette smoke induce structural and numerical chromosomal aberrations in vitro and could potentially increase levels of aneusomy in sperm. Moreover, increased levels of aneusomy in sperm are correlated with low implantation rates, spontaneous abortions and fetal losses. Studies of chromosome 3 in sperm suggest it may be more prone to segregation anomalies than other autosomes, but there has been no systematic investigation of the incidence of disomy for chromosome 3 in sperm derived from donor male smokers. The objective of this study was to use FISH to evaluate the influence of smoking on the levels of disomy for chromosomes X and Y, and to determine whether disomy levels for chromosome 3 were elevated in sperm derived from male smokers.ResultsFISH analysis was used to evaluate the frequency of disomies of chromosomes 3, X, and Y in sperm of 10 smokers, compared to a control group of 7 non-smoking fertile men. All the subjects presented a normal somatic karyotype. There was a significant increase in the overall frequency of disomies in sperm derived from the smoking group (P< 0.0001). When each chromosome pair was analyzed individually, disomy of chromosome 3 in smokers was found to be more than twice that observed in the matched non-smoker control group. In addition we observed a higher frequencies of disomy of the X and Y chromosomes, indicating elevated levels of diploidy in the sperm from the smoking group.ConclusionsIn this study we have shown that chromosome 3 may be susceptible to smoking-related segregation anomalies. Our results also suggest that errors can occur in both meiosis I and II, confirming the emerging literature that the male meiotic process may generally be affected by the genotoxic damage from tobacco use. Collectively, these findings provide additional evidence for enhancing tobacco control measures, and suggest that FISH analysis of chromosome 3 in sperm may be useful for monitoring smoking–induced segregation damage as part of the evaluation of infertile males.

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The genotoxic effect of nicotine on chromosomes of human fetal cells: The first report described as an important study

Abstract: Results of this study confirm that the nicotine leads to significant direct genotoxic effects in human fetal cells in vitro. We speculate that there is an association between prenatal exposure to cigarette smoke and in utero aneuploidies.

Cited by 25 publications

References 39 publications

The standardized herbal formula, PM014, ameliorated cigarette smoke-induced lung inflammation in a murine model of chronic obstructive pulmonary disease

The standardized herbal formula, PM014, ameliorated cigarette smoke-induced lung inflammation in a murine model of chronic obstructive pulmonary disease

Electronic Cigarettes. A Position Statement of the Forum of International Respiratory Societies

Smoking-induced chromosomal segregation anomalies identified by FISH analysis of sperm

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