The General Transcription Factor TAF7 Is Essential for Embryonic Development but Not Essential for the Survival or Differentiation of Mature T Cells

Gégonne, Anne; Tai, Xuguang; Zhang, Jinghui; Wu, Gang; Zhu, Jianjian; Yoshimoto, Aki; Hanson, Jeffrey C.; Cultraro, Constance M.; Chen, Qing Rong; Guinter, Terry I.; Zhang, Yang; Hathcock, Karen S.; Singer, Alfred; Rodriguez‐Canales, Jaime; Tessarollo, Lino; Mackem, Susan; Meerzaman, Daoud; Buetow, Ken H.; Singer, Dinah S.

doi:10.1128/mcb.06305-11

Cited by 44 publications

(67 citation statements)

References 39 publications

(45 reference statements)

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“…Preliminary findings indicate that this is the case: In a variety of cells, there is a molar excess is embryonic lethal between 4.5 and 5.5 d post coitus, the time at which the embryo implants and undergoes marked cellular expansion. 26 Taken together, these findings suggest that all cells have a requirement for TAF7 to proliferate.…”

Section: Taf7 Regulates the Major Rnap II Ctd Kinasesmentioning

confidence: 78%

“…TAF7-/-MEFs are blocked throughout cell cycle. 26 Furthermore, overexpression of TAF7 in MEFs also does not cause a specific cell cycle arrest (unpublished data). Thus, although TAF7 interacts with TAF1 and BRD4, mutation or deletion of either causes an arrest in cell cycle at the G1/S interface, TAF7 deletion does not have a similar effect.…”

Section: Taf7 Regulates the Major Rnap II Ctd Kinasesmentioning

confidence: 89%

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Taf7

Gégonne

Devaiah²,

Singer³

2013

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Section: Taf7 Regulates the Major Rnap II Ctd Kinasesmentioning

confidence: 78%

Section: Taf7 Regulates the Major Rnap II Ctd Kinasesmentioning

confidence: 89%

Taf7

Gégonne

Devaiah²,

Singer³

2013

Transcription

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“…Because lck-cre + mice differ from WT mice, care should also be taken when interpreting results using only an lck-cre + control. Intriguingly, some investigators reported no differences between lck-cre + or lck-cre 2 mice, whereas others did (17,19,21,(29)(30)(31)(32)(33)(35)(36)(37) (Fig. 6).…”

Section: Discussionmentioning

confidence: 91%

lck-Driven Cre Expression Alters T Cell Development in the Thymus and the Frequencies and Functions of Peripheral T Cell Subsets

Carow

Gao

Coquet

et al. 2016

The Journal of Immunology

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Conditional gene targeting using the bacteriophage-derived Cre recombinase is widely applied for functional gene studies in mice. Mice transgenic for Cre under the control of the lck gene promoter are used to study the role of loxP-targeted genes in T cell development and function. In this article, we show a striking 65% reduction in cellularity, preferential development of γδ versus αβ T cells, and increased expression of IL-7R in the thymus of mice expressing Cre under the proximal lck promoter (lck-cre+ mice). The transition from CD4/CD8 double-negative to double-positive cells was blocked, and lck-cre+ double-positive cells were more prone to apoptosis and showed higher levels of Cre expression. Importantly, numbers of naive T cells were reduced in spleens and lymph nodes of lck-cre+ mice. In contrast, frequencies of γδ T cells, CD44+CD62L− effector T cells, and Foxp3+ regulatory T cells were elevated, as was the frequency of IFN-γ–secreting CD4+ and CD8+ T cells. A literature survey of 332 articles that used lck-cre+ mice for deletion of floxed genes indicated that results are statistically influenced by the control used (lck-cre+ or lck-cre−), more frequently resembling the lck-cre+ phenotype described in this article if lck-cre− controls were used. Altogether, care should be taken when interpreting published results and to properly control targeted gene deletions using the lck-cre+ strain.

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“…TAF expression is lost in some differentiated cell types, including hepatocytes, myotubes, and Drosophila wings (13)(14)(15). Although the canonical TAFs are essential for proliferating cells, they are not essential for survival or even proliferation-independent differentiation of postmitotic cells (16,17). Consistent with this finding, depletion or inactivation of some of the TAFs causes cell-cycle arrest (18)(19)(20)(21).…”

mentioning

confidence: 74%

Holo-TFIID controls the magnitude of a transcription burst and fine-tuning of transcription

Pennington

Marr

Chirn

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Transcription factor (TF)IID is a central player in activated transcription initiation. Recent evidence suggests that the role and composition of TFIID are more diverse than previously understood. To investigate the effects of changing the composition of TFIID in a simple system, we depleted TATA box-binding protein-associated factor (TAF)1 from Drosophila cells and determined the consequences on metal-induced transcription at an inducible gene, metallothionein B. We observe a marked increase in the levels of both the mature message and pre-mRNA in TAF1-depleted cells. Under conditions of continued metal exposure, we show that TAF1 depletion increases the magnitude of the initial transcription burst but has no effect on the timing of that burst. We also show that TAF1 depletion causes delay in the shutoff of transcription upon removal of the stimulus. Thus, TAFs are involved in both establishing an upper limit of transcription during induction and efficiently turning the gene off once the inducer is removed. Using genome-wide nascent sequencing, we identify hundreds of genes that are controlled in a similar manner, indicating that the findings at this inducible gene are likely generalizable to a large set of promoters. There is a long-standing appreciation for the importance of the spatial and temporal control of transcription. Here we uncover an important third dimension of control: the magnitude of the response. Our results show that the magnitude of the transcriptional response to the same signaling event, even at the same promoter, can vary greatly depending on the composition of the TFIID complex in the cell.heavy metal | MtnA | coactivator | RNA polymerase

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The General Transcription Factor TAF7 Is Essential for Embryonic Development but Not Essential for the Survival or Differentiation of Mature T Cells

Cited by 44 publications

References 39 publications

Taf7

Taf7

lck-Driven Cre Expression Alters T Cell Development in the Thymus and the Frequencies and Functions of Peripheral T Cell Subsets

Holo-TFIID controls the magnitude of a transcription burst and fine-tuning of transcription

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