The gene coding for a new transcription factor (ftf1) of Fusarium oxysporum is only expressed during infection of common bean

Ramos, Brisa; Alves-Santos, Fernando M.; García-Sánchez, M. Asunción; Martín-Rodrigues, Noemí; Eslava, Arturo P.; Díaz-Mínguez, José María

doi:10.1016/j.fgb.2007.03.003

Cited by 54 publications

(90 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This range of variability, together with the finding that some paralogues are probably not functional because of structural reasons (this work), suggests that the FTF gene family has evolved by gene duplication of an ancestral unique gene, most probably FTF2, and the subsequent accumulation of mutations in the duplicated copies. The FTF1 paralogues are located in LS regions enriched in transposons (Ma et al, 2010;Ramos et al, 2007;Schmidt et al, 2013), whose movement could have originated the duplications. The data presented here demonstrate that some of these mutations have determined a functional specialization of the FTF1 paralogues, which is closely related to the ability to colonize the vascular system.…”

Section: Discussionmentioning

confidence: 99%

“…phaseoli harboured four and five copies of this gene, respectively (Ramos et al, 2007;de Vega-Bartol et al, 2011). However, a faint hybridization signal could also be detected in digested DNA of these strains, non-pathogenic and weakly virulent (WV, when inoculated in common bean plants) strains (Fig.…”

Section: Ftf2 Is a Single Copy Gene With Similarity To Ftf1mentioning

confidence: 94%

“…phaseoli (Ramos et al, 2007) as part of a SCAR (Sequence Characterized Amplified Region) designed for in planta detection (Alves-Santos et al, 2002b). mRNA transcribed from the multiple copies of FTF1, or at least some of them, is abundant in plants colonized by F. oxysporum, but barely detectable in cultures of the fungus (Ramos et al, 2007). The expression level of the gene correlates with the number of copies in strains of F. oxysporum f. sp.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The FTF gene family regulates virulence and expression of SIX effectors in Fusarium oxysporum

Niño-Sánchez

Castillo

Tello

et al. 2016

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

SUMMARYThe FTF (Fusarium transcription factor) gene family comprises a single copy gene, FTF2, which is present in all the filamentous ascomycetes analysed, and several copies of a close relative, FTF1, which is exclusive to Fusarium oxysporum. An RNAmediated gene silencing system was developed to target mRNA produced by all the FTF genes, and tested in two formae speciales: F. oxysporum f. sp. phaseoli (whose host is common bean) and F. oxysporum f. sp. lycopersici (whose host is tomato). Quantification of the mRNA levels showed knockdown of FTF1 and FTF2 in randomly isolated transformants of both formae speciales. The attenuation of FTF expression resulted in a marked reduction in virulence, a reduced expression of several SIX (Secreted In Xylem) genes, the best studied family of effectors in F. oxysporum, and lower levels of SGE1 (Six Gene Expression 1) mRNA, the presumptive regulator of SIX expression. Moreover, the knockdown mutants showed a pattern of colonization of the host plant similar to that displayed by strains devoid of FTF1 copies (weakly virulent strains). Gene knockout of FTF2 also resulted in a reduction in virulence, but to a lesser extent. These results demonstrate the role of the FTF gene expansion, mostly the FTF1 paralogues, as a regulator of virulence in F. oxysporum and suggest that the control of effector expression is the mechanism involved.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Ftf2 Is a Single Copy Gene With Similarity To Ftf1mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The FTF gene family regulates virulence and expression of SIX effectors in Fusarium oxysporum

Niño-Sánchez

Castillo

Tello

et al. 2016

Molecular Plant Pathology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Molecular markers based on the genetic determinants of pathogenicity are more likely to succeed in distinguishing formae speciales (van der Does et al, 2008). Such is the case of the gene targeted by the A280-B310 primers developed by Alves-Santos et al (2002b) that encodes a transcription factor from highly virulent Fop strains, expressed during early stages of host infection and located in a small chromosome (Ramos et al, 2007). These primers failed with three Fop strains in the present study, Fop 11, 12, and 13, that induce milder symptoms and resemble the weakly virulent strains described by Alves-Santos et al (2002b) that did not result in amplification product with the same primers.…”

Section: Discussionmentioning

confidence: 99%

“…lycopersici SIX genes (Ramos et al, 2007;van der Does et al, 2008;Vega-Bartol et al, 2011) are concentrated in lineage specific entire chromosomes and chromosome segments of F. oxysporum that can be horizontally transferred between strains with distinct genetic background, transforming in a single event a non-pathogenic indigenous soilborne F. oxysporum into a highly aggressive and locally adapted host-specific formae EF-1α gene and IGS rDNA sequencing of Fusarium oxysporum f. sp. vasinfectum... Moricca et al (1998) to strains used in this study and to others with sequences deposited in the GenBank.…”

Section: Discussionmentioning

confidence: 99%

EF-1α gene and IGS rDNA sequencing of Fusarium oxysporum f. sp. vasinfectum and F. oxysporum f. sp. phaseoli reveals polyphyletic origin of strains

Silva

Vechiato

Harakava

2014

Trop. plant pathol.

View full text Add to dashboard Cite

The fungi Fusarium oxysporum f. sp. vasinfectum (Fov) and F. oxysporum f. sp. phaseoli (Fop) are important pathogens that cause wilt on cotton and common bean, respectively. In the present study, phylogenetic trees constructed with partial sequences of the translation elongation factor gene and ribosomal intergenic spacer region of Brazilian Fov and Fop strains revealed polyphyletic origin of strains within both formae speciales. This creates an obstacle for the development of specific molecular diagnostic methods and explains the failure of supposedly specific methods described in the literature to correctly discriminate formae speciales of F. oxysporum.

show abstract

Fusarium wilt–common bean pathosystem: Pathogen variability and genetic control

et al. 2023

View full text Add to dashboard Cite

Sustainable management of plant diseases has been a challenge in the effort to feed a human population that has increased substantially. One of the main diseases that affect common bean and dry bean is Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli (Fop), which can cause losses of up to 100% in crop production. The Fop infection process starts in the roots, followed by colonization and obstruction of xylem vessels, leading to leaf wilting, vascular discoloration, chlorosis, dwarfism, and premature plant death. Fop show high genetic variability, and seven different physiological races have been reported in different regions of the world. There is also a study in which the formae specialis designation has been replaced by Fusarium species. Different studies in common bean have shown that physical, biological, and chemical changes are involved in the Fop resistance mechanism and in the Fusarium and common bean pathosystem. Many studies have been conducted to detect candidate genes and pathways associated with resistance to Fop. This work offers useful information for advancing common bean resistance breeding.

show abstract

The gene coding for a new transcription factor (ftf1) of Fusarium oxysporum is only expressed during infection of common bean

Cited by 54 publications

References 55 publications

The FTF gene family regulates virulence and expression of SIX effectors in Fusarium oxysporum

The FTF gene family regulates virulence and expression of SIX effectors in Fusarium oxysporum

EF-1α gene and IGS rDNA sequencing of Fusarium oxysporum f. sp. vasinfectum and F. oxysporum f. sp. phaseoli reveals polyphyletic origin of strains

Fusarium wilt–common bean pathosystem: Pathogen variability and genetic control

Contact Info

Product

Resources

About

The gene coding for a new transcription factor (ftf1) of Fusarium oxysporum is only expressed during infection of common bean

Cited by 54 publications

References 55 publications

The FTF gene family regulates virulence and expression of SIX effectors in Fusarium oxysporum

The FTF gene family regulates virulence and expression of SIX effectors in Fusarium oxysporum

EF-1&#945; gene and IGS rDNA sequencing of Fusarium oxysporum f. sp. vasinfectum and F. oxysporum f. sp. phaseoli reveals polyphyletic origin of strains

Fusarium wilt–common bean pathosystem: Pathogen variability and genetic control

Contact Info

Product

Resources

About

EF-1α gene and IGS rDNA sequencing of Fusarium oxysporum f. sp. vasinfectum and F. oxysporum f. sp. phaseoli reveals polyphyletic origin of strains