The functional assessment of autotransplanted splenic tissue by its capacity to remove oxidatively modified erythrocytes

The Effects of Splenectomy and Autologous Spleen Transplantation on Complete Blood Count and Cell Morphology in a Porcine Model

“…This founding is in accordance with the results of previous studies on various species, so we can conclude these changes were specific for splenectomized patients. Some authors use the number of erythrocytes containing Howell-Jolly bodies to assess preservation of spleen's blood filtering function (Patel et al, 1981;Resende & Petroianu, 2003;Resende et al, 2002), but number of oxidatively modified erythrocytes containing Heinz bodies can also be used for that purpose (Haklar et al, 1997). Polychromasia and increased number of circulating erythroblasts (Picture 2.)…”

“…There is still controversy about the effectiveness of regenerated splenic tissue, but the one conclusion of all researches done is common, that functionality and histological restitution of the transplanted splenic tissue depends on the amount of successfully transplanted mass of spleen (Haklar et al, 1997;Sipka, et al, 2006;Tang et al, 2003). In the present research the implant in overall amount of 20 % has not been enough for keeping filtration function of healthy spleen, which correlates with researches from Tang et al (2003), who find that architecture of red and white pulp, as well as restitution of cardiovascular system is not sufficient for another seven months after transplantation.…”

“…However, total removal of the spleen may lead to side effects such as postsplenectomy infections and sepsis, due to the decreased production of antibodies and phagocytes or thrombosis, due to elevated platelet count in blood (Bessler et al, 2004;Khan et al, 2009;Miko et al, 2003;Timens & Leemans, 1992). Also, many studies report increased count of morphologically abnormal erythrocytes, immature red blood cells and pathologic erythrocyte inclusions in the peripheral blood of various species following splenectomy as a result of the loss of splenic filtrating function (Haklar et al, 1997;Resende et al, 2002;Traub et al, 1987). In addition, it has been reported that removal of the spleen causes significantly higher increase of reticulocyte count than other surgeries.…”

Cirurgias conservadoras do baço para tratamento da doença de Gaucher

“…32,35,37,42,51,59 Por outro lado, trabalhos experimentais foram unânimes em encontrar menor incidência e intensidade de sepse em animais submetidos ao auto-implante, quando comparados com os asplênicos. 34,38,40,41,44,45 Atualmente, acredita-se que os resultados não favoráveis descritos na literatura decorrem principalmente da quantidade insuficiente de tecido esplênico implantado e que, apesar de sua função estar presente, ela não pode ser detectada. 48,50,53 De qualquer modo, os auto-implantes, mesmo quando a quantidade de tecido é maior, apresentam eficácia funcional menor do que a evidenciada pelos remanescentes das esplenectomias parciais e subtotais.…”

“…Estudos recentes mostraram que os auto-implantes são eficazes em manter pelo menos parte das funções do baço. [32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47] O presente trabalho apresenta nossa experiência com a conservação esplênica parcial, por meio de esplenectomia subtotal ou auto-implantes esplênicos, em pacientes com doença de Gaucher, em que foi indicada operação esplenorredutora.…”