The function of mitochondrial FOF1 ATP-synthase from the whiteleg shrimp Litopenaeus vannamei muscle during hypoxia

Martínez‐Cruz, Oliviert; Barca, Ana M. Calderón de la; Uribe‐Carvajal, Salvador; Muhlia‐Almazán, Adriana

doi:10.1016/j.cbpb.2012.03.004

Cited by 20 publications

(11 citation statements)

References 37 publications

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“…Taken together, the results indicated that expression of shrimp genes led to the assembly of a functionally active F 1 -ATPase but at least in the case of Pv ATP2 it did not support respiratory growth, probably due to a defective interaction with the stalk or F 0 sectors. Interestingly, a recent publication has revealed an increase in the ATP hydrolyzing activity in muscle mitochondria of shrimp under hypoxia condition [30]. These data raise an intriguing question as to whether native shrimp F 1 has evolved a robust ATP hydrolyzing activity independent of F 0 , capable of maintaining membrane potential and providing some capacity for shrimp to survive under low oxygen conditions (partial hypoxia), as opposed to survival under complete hypoxia that has evolved in the petite-positive yeast.…”

Section: Resultsmentioning

confidence: 99%

Shrimp ATP synthase genes complement yeast null mutants for ATP hydrolysis but not synthesis activity

et al. 2012

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The aim of this study was to examine the feasibility of employing a yeast functional complementation assay for shrimp genes by using the shrimp mitochondrial F1F0-ATP synthase enzyme complex as a model. Yeast mutants defective in this complex are typically respiratory-deficient and cannot grow on non-fermentable carbon sources such as glycerol, allowing easy verification of functional complementation by yeast growth on media with them as the only carbon source. We cloned the previous published sequence of ATP2 (coding for ATP synthase β subunit) from the Pacific white shrimp Penaeus vannamei (Pv) and also successfully amplified a novel PvATP3 (coding for the ATP synthase γ subunit). Analysis of the putative amino acid sequence of PvATP3 revealed a significant homology with the ATP synthase γ subunit of crustaceans and insects. Complementation assays were performed using full-length ATP2 and ATP3 as well as a chimeric form of ATP2 containing a leader peptide sequence from yeast and a mature sequence from shrimp. However, the shrimp genes were unable to complement the growth of respective yeast mutants on glycerol medium, even though transcriptional expression of the shrimp genes from plasmid-borne constructs in the transformed yeast cells was confirmed by RT-PCR. Interestingly, both PvATP2 and PvATP3 suppressed the lethality of the yeast F1 mutants after the elimination of mtDNA, which suggests the assembly of a functional F1 complex necessary for the maintenance of membrane potential in the ρ0 state. These data suggest an incompatibility of the shrimp/yeast chimeric F1-ATPase with the stalk and probably also the F0 sectors of the ATP synthase, which is essential for coupled energy transduction and ATP synthesis.

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Section: Resultsmentioning

confidence: 99%

Shrimp ATP synthase genes complement yeast null mutants for ATP hydrolysis but not synthesis activity

et al. 2012

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“…Pi (Futai et al 1989;Lai-Zhang and Mueller 2000). The mitochondrial ATPase activity of shrimp is promoted during environmental stress conditions then, a specific mechanism may exist to regulate the activity of the enzyme (Martinez-Cruz et al 2012). F 1 -ATP synthase beta similar to BP53 in L. vannamei is associated to the process of WSS viral infection.…”

Section: Discussionmentioning

confidence: 98%

Differential expression of proteins in the gills of Litopenaeus vannamei infected with white spot syndrome virus

2014

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Determination of differentially expressed protein profile is necessary to understand the host response to viral infection. Proteomics can be applied as a tool to examine white shrimp Litopenaeus vannamei molecular responses against white spot syndrome virus (WSSV) infection, thus enabling development of effective strategies to reduce their impact on farms. In the present study, specific pathogen-free shrimp was tested against WSSV infection under several time intervals. Shrimps were submitted to a viral load of with 5.5 9 10 6 viral copies in 100 lL/shrimp. The monitoring of infection was performed in intervals of 6, 12, 24, 48 and 72 h after infection. The analysis was realized using 2-DE, and differentially expressed proteins were identified by MALDI-TOF mass spectrometry (MS) peptide mass fingerprint (PMF). Between the differentially expressed proteins found in the infected animals, the most important were identified as caspase-2, ubiquitin and F1-ATP synthase. They are interesting candidates for biomarkers because could be related to the beginning of apoptosis process. The differentially expressed protein profile creates a new paradigm in the analysis of L. vannamei shrimp molecular response to WSSV infection and in virus-host relationship. Furthermore, it proposes potential biomarkers that allow strategies both selecting less susceptible individuals and reducing the impact of viruses on farms.

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“…The opening of the mitochondrial permeability transition pore (MPTP) leads to mitochondrial uncoupling and to the activation of signaling events leading to apoptosis [56], which was first detected in mammalian mitochondria as a response to the disruption of intracellular calcium homeostasis. In crustaceans subjected to hypoxia, mitochondrial functions are downregulated [57,58,20] and there is an anoxia-triggered intracellular increase in both calcium and phosphate, while ATP production is inhibited, probably as a result of the opening of a MPTP. In Artemia franciscana [26] and in the ghost shrimp Lepidophthalmus louisianensis [59], the proteins needed to form the MPTP are present.…”

Section: (A) Proton Sinksmentioning

confidence: 99%

Oxygen: From Toxic Waste to Optimal (Toxic) Fuel of Life

Rosas‐Lemus¹,

Uribe‐Alvarez²,

Contreras‐Zentella³

et al. 2016

Free Radicals and Diseases

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Some . billion years ago, the great oxygenation event (GOE) led to a -fold rise in atmospheric oxygen [O ], killing most species on Earth. In spite of the tendency to produce toxic reactive oxygen species (ROS), the highly exergonic reduction of O made it the ideal biological electron acceptor. During aerobic metabolism, O is reduced to water liberating energy, which is coupled to adenosine triphosphate (ATP) synthesis. Today, all organisms either aerobic or not need to deal with O toxicity. O -permeant organisms need to seek adequate [O ], for example, aquatic crustaceans bury themselves in the sea botom where O is scarce. Also, the intestinal lumen and cytoplasm of eukaryotes is a microaerobic environment where many facultative bacteria or intracellular symbionts hide from oxygen. Organisms such as plants, ish, reptiles and mammals developed O -impermeable epithelia, plus specialized external respiratory systems in combination with O -binding proteins such as hemoglobin or leg-hemoglobin control [O ] in tissues. Inside the cell, ROS production is prevented by rapid O consumption during the oxidative phosphorylation (OxPhos) of ATP. When ATP is in excess, OxPhos becomes uncoupled in an efort to continue eliminating O . Branched respiratory chains, unspeciic pores and uncoupling proteins (UCPs) uncouple OxPhos. One last line of resistance against ROS is deactivation by enzymes such as super oxide dismutase and catalase. Aerobic organisms proit from the high energy released by the reduction of O , while at the same time they need to avoid the toxicity of ROS.

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The function of mitochondrial FOF1 ATP-synthase from the whiteleg shrimp Litopenaeus vannamei muscle during hypoxia

Cited by 20 publications

References 37 publications

Shrimp ATP synthase genes complement yeast null mutants for ATP hydrolysis but not synthesis activity

Shrimp ATP synthase genes complement yeast null mutants for ATP hydrolysis but not synthesis activity

Differential expression of proteins in the gills of Litopenaeus vannamei infected with white spot syndrome virus

Oxygen: From Toxic Waste to Optimal (Toxic) Fuel of Life

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