The Forkhead Transcription Factor FOXP2 Is Required for Regulation of p21WAF1/CIP1 in 143B Osteosarcoma Cell Growth Arrest

Gascoyne, Duncan M.; Spearman, Hayley; Lyne, Linden; Puliyadi, Rathi; Perez-Alcantara, Marta; Coulton, Les; Fisher, Simon E.; Banham, Alison H.

doi:10.1371/journal.pone.0128513

Cited by 24 publications

(25 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, higher levels of expressions of GRIAL1, SGOL1 and Igfbp6 were found within parietal bone that displayed anti-proliferative feature, such as overexpression of GRIAL1 is anti-proliferative [28], and SGOL1 and Igfbp6 are involved in cell apoptosis [29-31]. Foxp2 can increase cell differentiation without affecting cell proliferation and cell survival [32], and transient upregulation of Foxp2 in pre-osteoblast mesenchymal cells regulates a p21-dependent growth arrest checkpoint [33]. Our findings are consistent with the publication that osteoblasts from the neural crest-derived frontal bone are less differentiated, grow faster compared to parietal bone-derived osteoblasts [3].…”

Section: Discussionmentioning

confidence: 99%

Physiological Signatures of Dual Embryonic Origins in Mouse Skull Vault

Zhao

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: The mammalian skull vault is a highly regulated structure and consists of several membrane bones of different tissue origins (e.g. neural crest derived frontal bone and mesoderm derived parietal bone). Although membrane bones form through intramembranous ossification, neural crest derived frontal bone has superior osteoblast activity and bone regeneration ability, triggering a novel conception for craniofacial reconstruction and bone regeneration called endogenous calvarial regeneration. However, a comprehensive landscape of the genes and signaling pathways involved in this process is not clear. Methods: Transcriptome analysis within the two bone elements is firstly performed to determine the physiological signatures of differential gene expressions in mouse skull vault. Results: Frontal bone tissues and parietal bone tissues maintain tissue origin through special gene expression similar to neural crest vs mesoderm tissue, and physiological functions between these two tissues are also found in differences related to proliferation, differentiation and extracellular matrix production and clustered signaling pathways. Conclusion: Our data provide novel insights into the potential gene regulatory network in regulating the development of neural crest-derived frontal bone and mesoderm-derived parietal bone.

show abstract

Section: Discussionmentioning

confidence: 99%

Physiological Signatures of Dual Embryonic Origins in Mouse Skull Vault

Zhao

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Emerging evidence has indicated that the expression of FOX genes, including FOXC2 27 , FOXF1 28 , FOXL1 29 , FOXM1 30 , 31 , FOXO1 32 , 33 and FOXP2 34 , is disrupted in osteosarcoma cells and primary tumor biopsy samples. As shown in Table 1 , according to their expression in osteosarcoma cells and primary tumor biopsy samples, these FOX genes can be divided into two groups: oncogenes and anti-oncogenes.…”

Section: Expression Of Fox Genes In Osteosarcomamentioning

confidence: 99%

“…Oncogenes primarily include FOXC2 , FOXM1 and FOXP2 , and the elevated expression level of these genes has been observed in osteosarcoma cells, which is positively correlated with tumor size, clinical stage, pathological facture, and distant metastasis 27 - 29 . Anti-oncogenes primarily include FOXF1 , FOXL1 and FOXO1 , and decreased or absent expression levels of these genes were examined in osteosarcoma cells and primary tumor biopsy samples 30 - 34 .…”

Section: Expression Of Fox Genes In Osteosarcomamentioning

confidence: 99%

“…Recently, Gascoyne et al identified both FOXP2 mRNA and protein levels were highly expressed in an osteosarcoma cell line (Saos-2) 34 . Treatment with a combination of Vitamin D3 and TGF-β on MG-63 osteosarcoma cells resulted in cell growth arrest at sub-confluence and induction of alkaline phosphatase activity, but not induction of FOXP2 expression 34 . However, the FOXP2 mRNA levels were extremely elevated when MG-63 cells were treated with vehicle 34 .…”

Section: Roles Of Fox Genes In Osteosarcomamentioning

confidence: 99%

See 1 more Smart Citation

The Emerging Roles of Forkhead Box (FOX) Proteins in Osteosarcoma

Zhang¹,

Duan²,

Song³

et al. 2017

J. Cancer

View full text Add to dashboard Cite

Osteosarcoma is the most common bone cancer primarily occurring in children and young adults. Over the past few years, the deregulation of a superfamily transcription factors, known as forkhead box (FOX) proteins, has been demonstrated to contribute to the pathogenesis of osteosarcoma. Molecular mechanism studies have demonstrated that FOX family proteins participate in a variety of signaling pathways and that their expression can be regulated by multiple factors. The dysfunction of FOX genes can alter osteosarcoma cell differentiation, metastasis and progression. In this review, we summarized the evidence that FOX genes play direct or indirect roles in the development and progression of osteosarcoma, and evaluated the emerging role of FOX proteins as targets for therapeutic intervention.

show abstract

“…On the one hand, high expression of FOXP2 has been associated with a bad prognosis in prostate cancer [10], neuroblastoma [11] and multiple mieloma [12]. On the other hand, other studies have reported an association between FOXP2 expression and a better prognosis in breast cancer [13], liver cell carcinoma [14], osteosarcoma [15] and gastric cancer [16]. Thus, whether FOXP2 is a tumor suppressor or tumor-promoting gene is matter of controversy.…”

Section: Introductionmentioning

confidence: 99%

Low Expression of FOXP2 is Associated with Better Prognosis in Glioblastoma.

Plata‐Bello

Fariña-Jerónimo

Betancor

2020

Preprint

View full text Add to dashboard Cite

FOXP2 expression has been associated with the prognosis of some tumors, but the role of FOXP2 in glioblastoma has not been studied in-depth until now. The aim of the present work is to study the role of FOXP2 as a prognostic biomarker in glioblastoma.This is a retrospective observational case series study in which the expression of FOXP2 has been analyzed both at the protein level (immunohistochemistry) and at the mRNA level (RNAseq, in a cohort of glioblastoma patients from The Cancer Genome Atlas [TCGA] database). Other molecular and clinical data have also been included in the study, with special focus on miRNA expression data.Survival analysis using Log-Rank test and COX-regression have been used. Non-parametric statistical tests were also used to study differences between low and high FOXP2 expression groups.Patients with a high expression of FOXP2 protein showed a worse prognosis than those patients with low expression in both, progression free survival (PFS) (HR=1.711; p=0.034) and overall survival (OS) (HR=1.809;p=0.014). These associations were still statistically significant in multivariate analysis.No prognostic association was found with FOXP2 RNA expression. Interestingly, two miRNAs that target FOXP2 (hsa-miR-181a-2-3p and hsa-miR-20a-3p) showed an interaction effect on OS with FOXP2 expression. A low level of these miRNAs expression was associated with a significantly worse prognosis in patients with high FOXP2 RNA expression.Higher expression of FOXP2 at the protein level is associated with a worse prognosis. This protein expression may be regulated by the expression of specific miRNAs that target FOXP2 mRNA: hsa-miR-181a-2-3p and hsa-miR-20a-3p.

show abstract

The Forkhead Transcription Factor FOXP2 Is Required for Regulation of p21WAF1/CIP1 in 143B Osteosarcoma Cell Growth Arrest

Cited by 24 publications

References 48 publications

Physiological Signatures of Dual Embryonic Origins in Mouse Skull Vault

Physiological Signatures of Dual Embryonic Origins in Mouse Skull Vault

The Emerging Roles of Forkhead Box (FOX) Proteins in Osteosarcoma

Low Expression of FOXP2 is Associated with Better Prognosis in Glioblastoma.

Contact Info

Product

Resources

About