The Fluorophore 4′,6‐Diamidino‐2‐phenylindole (DAPI) Induces DNA Folding in Long Double‐Stranded DNA

Beccia, Maria Rosa; Biver, Tarita; Pardini, Alberto; Spinelli, Jacopo; Secco, Fernando; Venturini, Marcella; Busto, Natalia; López‐Cornejo, Pilar; Herrera, Victoria Isabel Martin; Prado‐Gotor, Rafael

doi:10.1002/asia.201200177

Cited by 34 publications

(23 citation statements)

References 65 publications

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“…The latter interaction can stabilize the less elongated polynucleotide conformation, giving rise to a decrease in the viscosity. 43 In contrast, the behavior is strikingly different at high R ratios (R > 0.5) for 12-s-12 series. Note that for the 16-s-16 the high R ratios cannot be explored due to the fact that these surfactants show much lower CMC values compared to the 12-s-12 series (see Table S1 †).…”

Section: Discussionmentioning

confidence: 92%

DNA conformational changes induced by cationic gemini surfactants: the key to switching DNA compact structures into elongated forms

et al. 2015

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Section: Discussionmentioning

confidence: 92%

DNA conformational changes induced by cationic gemini surfactants: the key to switching DNA compact structures into elongated forms

et al. 2015

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“…Hence, such interaction is entropically favourable. 4′,6-diamidino-2-phenylindole (DAPI) is a minor grove binder that bind to A-T rich region in DNA and is also used as DNA tag due to its fluorescent nature [ 14 ]. A single compound may utilise more than one mode of DNA binding (e.g., intercalation and groove binding) [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and viscometric determination of DNA-binding modes of some bioactive dibenzodioxins and phenazines

Phadte

Banerjee

Mate

et al. 2019

Biochemistry and Biophysics Reports

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Push-pull dibenzodioxins and phenazines having ‘anthracene-like’ planar structures and good charge transfer character had been previously synthesised in our laboratory. The dibenzodioxins had earlier proven their anti-proliferative nature against HeLa tumor cell lines. Since phenazines are structural analogues of the former, these molecules were evaluated in course of the current study for their cytotoxic action against HeLa cell lines and they exhibited strong anti-tumor activity. This behavior could be related to their good DNA binding property. The DNA binding modes of molecules 1 – 4 (Fig. 1) were evaluated using various experimental techniques and they interacted with DNA in a non-covalently by both intercalative as well as groove binding mechanisms. Molecule 1 follows predominantly intercalative binding mode whereas molecules 2 and 3 have nearly equal and opposite preferences for both groove binding and intercalative modes. For molecule 4 , groove binding is preferred mode of binding to DNA. A rationale for such differential binding behaviour is provided based on the subtle structural differences in our synthesised dibenzodioxins and phenazines. Elucidation of the mode of a molecule-DNA-binding event is relevant for understanding the mechanism of action of these molecules and will help promote further research into designing better DNA targeting small molecules.

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“…The binding parameter of BSA-triamcinolone system was analyzed according to Benesi-Hildebrand equation (Equation 4) (18); .…”

Section: Fluorimetric Studymentioning

confidence: 99%

Investigation of Triamcinolone-Bovine Serum Albumin (BSA) Interaction by Spectroscopic Methods

Aydınoğlu

2020

Journal of the Turkish Chemical Society Section A: Chemistry

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The aim of the present study was investigate the interaction between bovine serum albumin and triamcinolone. For this purpose, the interaction between BSA and triamcinolone was evaluated by UV-Vis and fluorescence spectroscopy under different temperatures and different salt concentrations at physiological pH (7.4). The binding constant of BSA-Triamcinolone system were evaluated different temperature at constant (pH=7.4) and ionic strength (0.01 M). The binding constant dependence of binding constant on temperature was analyzed by Van't Hoff equation. The standard enthalpy change (ΔH) was 9.0 kcal/mol and standard entropy change (ΔS) was 54.1 cal/mol K. In addition, the effect of salt concentration investigated for BSA-Triamcinolone system at constant temperature (T=25 °C) and increasing salt concentration lead to decrement on the binding constant value. The obtained thermodynamic parameters indicate hydrophobic forces take major role in BSA-Triamcinolone interaction. The arousal of salt concentration prompted to diminution on affinity between Triamcinolone and BSA.

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The Fluorophore 4′,6‐Diamidino‐2‐phenylindole (DAPI) Induces DNA Folding in Long Double‐Stranded DNA

Cited by 34 publications

References 65 publications

DNA conformational changes induced by cationic gemini surfactants: the key to switching DNA compact structures into elongated forms

DNA conformational changes induced by cationic gemini surfactants: the key to switching DNA compact structures into elongated forms

Spectroscopic and viscometric determination of DNA-binding modes of some bioactive dibenzodioxins and phenazines

Investigation of Triamcinolone-Bovine Serum Albumin (BSA) Interaction by Spectroscopic Methods

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