The Flame Photometer for the Measurement of Sodium and Potassium in Biological Materials

Hald, Pauline M.

doi:10.1016/s0021-9258(17)31003-7

Cited by 146 publications

(19 citation statements)

References 2 publications

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“…The homogenate was collected by rinsing the tissue homogenizer with 5 mL of deionized water to obtain a final volume of 10 mL. From this volume, 5 mL were heated in a water bath at the boiling point for 20 min and then centrifuged for 5 min at 1625× g. The supernatant was collected and Na + and K + were quantified on a Flame Photometer (Carls Zeiss PF5 371777) [55]. For the quantification, 1 mM NaCl and 1 mM KCl were used as standards, corresponding to 100 AU.…”

Section: Na + /K + Quantificationmentioning

confidence: 99%

Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock

Rodríguez-Pupo

Pérez-Llano

Tinoco-Valencia

et al. 2021

JoF

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Aspergillus sydowii is a moderate halophile fungus extensively studied for its biotechnological potential and halophile responses, which has also been reported as a coral reef pathogen. In a recent publication, the transcriptomic analysis of this fungus, when growing on wheat straw, showed that genes related to cell wall modification and cation transporters were upregulated under hypersaline conditions but not under 0.5 M NaCl, the optimal salinity for growth in this strain. This led us to study osmolyte accumulation as a mechanism to withstand moderate salinity. In this work, we show that A. sydowii accumulates trehalose, arabitol, mannitol, and glycerol with different temporal dynamics, which depend on whether the fungus is exposed to hypo- or hyperosmotic stress. The transcripts coding for enzymes responsible for polyalcohol synthesis were regulated in a stress-dependent manner. Interestingly, A. sydowii contains three homologs (Hog1, Hog2 and MpkC) of the Hog1 MAPK, the master regulator of hyperosmotic stress response in S. cerevisiae and other fungi. We show a differential regulation of these MAPKs under different salinity conditions, including sustained basal Hog1/Hog2 phosphorylation levels in the absence of NaCl or in the presence of 2.0 M NaCl, in contrast to what is observed in S. cerevisiae. These findings indicate that halophilic fungi such as A. sydowii utilize different osmoadaptation mechanisms to hypersaline conditions.

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Section: Na + /K + Quantificationmentioning

confidence: 99%

Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock

Rodríguez-Pupo

Pérez-Llano

Tinoco-Valencia

et al. 2021

JoF

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“…The measurement of sodium concentration on a larger scale in clinical laboratories became possible since the emergence of the flame emission spectrometer (FES). In this technique, the sample solution is diluted and a fine mist of the diluted aliquot is blown on a flame [ 10 ]. The intensity of the light emitted is directly proportional to the concentration of sodium in the sample [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this technique, the sample solution is diluted and a fine mist of the diluted aliquot is blown on a flame [ 10 ]. The intensity of the light emitted is directly proportional to the concentration of sodium in the sample [ 10 ]. But in current clinical practice, most of the laboratories use indirect potentiometry using an ion-selective electrode that has a sodium selective membrane, which measures the potential generated across a sodium selective membrane, when immersed in a serum sample [ 5 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

Pseudohyponatremia Leading to a Fatal Outcome in a Patient With Familial Hypertriglyceridemia

Dawson¹,

Kanukuntla²,

Kata³

et al. 2021

Cureus

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Serum sodium assay is a commonly performed laboratory test in a clinical setting and the results are taken for granted without being aware of the actual methods involved. In conditions like hyperlipidemia and hyperproteinemia, excessive lipids in serum dilute the water component of the serum. Since sodium is dissolved only in the aqueous phase of serum, the sodium content per unit volume of plasma is reduced. Currently, most of the laboratories use the indirect ion-selective electrode method (ISE), where the plasma sample is diluted before the measurement. Indirect ISE may not give accurate results in conditions with higher serum lipid and protein levels. Overcorrection of the serum sodium levels in pseudohyponatremia may cause serious complications. We report a case of a 26-year-old Asian male with a past medical history of chronic pancreatitis, familial hypertriglyceridemia, and fatty liver who presented to the emergency department with acute pancreatitis. Initially, the patient was found to have hyponatremia and he was started on hypertonic saline for one day. Later the patient’s condition deteriorated and then it was determined that serum sodium results were a measurement artifact since the patient had extremely high levels of triglycerides. After realizing that it was a measurement artifact, the saline infusion was stopped and he was started on desmopressin. However, the patient deteriorated neurologically and expired later. As this patient had normal sodium levels, administration of hypertonic saline led to a fatal outcome.

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“…The soil pH was determined according to the electrometric method. Organic carbon was analyzed by the Walkley-Black procedure as described by Nelson and Sommers (1982), total N by the Kjeldahl method as described by KIT (1984), P by the Bray-1 method as described by Bray and Kurtz (1945), and K by the flame photometer method as described by Hald (1947). Particle size distribution was carried out by the hydrometer method as described by Bouyoucos (1962).…”

Section: Soil Sampling and Analysismentioning

confidence: 99%

Response of Peanut Varieties to Phosphorus and Rhizobium Inoculant Rates on Haplic Lixisols of Guinea Savanna Zone of Ghana

Yaro

Mahama

Kugbe

et al. 2021

Front. Sustain. Food Syst.

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Peanut forms a major component of the predominantly cereal-based farming systems in Northern Ghana. However, yields are low, prompting the need to evaluate the effects of phosphorus (PR) and rhizobium inoculant (IR) rates on growth, nodulation, and yield of peanut varieties. On-station and on-farm experiments were conducted to determine the interaction effects of three P rates (0, 30, and 60 kg P2O5/ha), three IR rates (0, 3, and 6 g/kg seed), and two peanut varieties [Chinese and Nkatie Savannah Agricultural Research Institute (SARI)] on growth, nodulation, and yield of peanut on Haplic Lixisols of Northern Ghana. Both experiments were conducted using a split-split plot design replicated three times for the on-station experiment and on six farmer's fields (on-farm experiment). In both experiments, combined application of 60 kg P2O5/ha and IR at 6 g/kg seed increased pod number in the Nkatie SARI and Chinese varieties compared to their control counterparts. PR × V interaction influenced growth, effective nodule number, and podding capacity with 60 kg P2O5/ha combined with Nkatie SARI to produce significantly higher values. The interaction of IR × V improved pod number, nodule number, and harvest index, such that inoculant at 6 g/kg seed combined with Nkatie SARI gave the best performance. PR × IR also had a significant interactive influence on peanut grain yield. Higher grain yields were recorded from 60 kg P2O5/ha in combination with 6 g/kg seed of rhizobium inoculant. Therefore, these results suggest that the use of P fertilizer at 60 kg/ha and rhizobium inoculant at 6 g/kg seed increase peanut productivity on Haplic Lixisols in Northern Ghana. However, it was prudent not to suggest any recommendations from the P rates in interaction with IR, since the result between the on-station and on-farm experiments appeared not consistent.

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The Flame Photometer for the Measurement of Sodium and Potassium in Biological Materials

Cited by 146 publications

References 2 publications

Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock

Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock

Pseudohyponatremia Leading to a Fatal Outcome in a Patient With Familial Hypertriglyceridemia

Response of Peanut Varieties to Phosphorus and Rhizobium Inoculant Rates on Haplic Lixisols of Guinea Savanna Zone of Ghana

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