The first analysis of Trichinella spiralis and Trichinella britovi adult worm excretory-secretory proteins by two-dimensional electrophoresis coupled with LC-MS/MS

Grzelak, Sylwia; Stachyra, Anna; Bień-Kalinowska, Justyna

doi:10.1016/j.vetpar.2020.109096

Cited by 9 publications

(10 citation statements)

References 44 publications

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“…Certain proteins (for example, putative onchocystatin) were involved in host-parasite interactions. Excretory–secretory proteins, as the most important products of host–parasite interaction, were investigated in the latest studies on T. spiralis , T. pseudospiralis and T. britovi [ 190 , 191 , 192 ].…”

Section: Discovery Approach—description Of the Selected Fbps And Tmentioning

confidence: 99%

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

Stryiński

Łopieńska–Biernat

Carrera

2020

Foods

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Foodborne parasitoses compared with bacterial and viral-caused diseases seem to be neglected, and their unrecognition is a serious issue. Parasitic diseases transmitted by food are currently becoming more common. Constantly changing eating habits, new culinary trends, and easier access to food make foodborne parasites’ transmission effortless, and the increase in the diagnosis of foodborne parasitic diseases in noted worldwide. This work presents the applications of numerous proteomic methods into the studies on foodborne parasites and their possible use in targeted diagnostics. Potential directions for the future are also provided.

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Section: Discovery Approach—description Of the Selected Fbps And Tmentioning

confidence: 99%

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

Stryiński

Łopieńska–Biernat

Carrera

2020

Foods

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“…Although T. spiralis has typically been regarded as the most significant pathogen related to public health, there is a need for further studies on the antigenic profile associated with T. britovi infection [ 30 ], which occurs more frequently among wild carnivores [ 1 , 12 , 31 , 32 ]. In natural environments, carnivorous animals play an important role in the transmission of Trichinella nematodes, including T. britovi .…”

Section: Introductionmentioning

confidence: 99%

“…Although in the domestic cycle the immune response during Trichinella infection has been widely studied in pigs, under controlled conditions with a known infection dose and species of Trichinella [ 14 , 30 , 36 , 37 , 38 , 39 ], the immunoprevalence of anti- Trichinella antibodies has been also examined in many animals species connected to the sylvatic cycle [ 13 , 24 , 26 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ] as the main reservoir of Trichinella nematodes in the environment. Infections in wildlife occur in a natural way—the dose of infection, the timing, and whether they occur once or repeatedly remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Immunoproteomic Analysis of Trichinella britovi Proteins Recognized by IgG Antibodies from Meat Juice of Carnivores Naturally Infected with T. britovi

Cybulska¹

2022

Pathogens

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Infection with Trichinella nematodes elicits non-specific and specific immune responses; these depend on the dose of infection, the nematode, and the host species. Few studies have examined the presence of specific antibodies against Trichinella spp. in the meat juice of wild animals. The aims of the study were to determine the prevalence of antibodies against Trichinella spp. in meat juice and to identify the specific proteins reacting with the meat juice from free-living carnivores naturally infected with the parasite. Meat juice samples were taken from foxes, badgers, raccoon dogs, and martens and tested with indirect ELISA. Antibodies against Trichinella spp. were detected in 10% of foxes and 46% of raccoon dogs. The ELISA results were confirmed by immunoblot, which revealed different protein patterns in meat juice from red foxes, raccoon dogs, and badgers. The most frequently observed bands were sent for further analysis by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) for the detection of Trichinella britovi immunogenic proteins. The results confirm the presence of proteins such as serine protease and heat shock proteins associated with Trichinella infection. These findings provide that meat juice is a useful matrix for proteomic analysis.

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“…Immunoreactive proteins that were used to diagnose T. spiralis infection were characterized by a combination of twodimensional electrophoresis , immunoblot, and liquid chromatography-tandem mass spectrometry (LC MS-MS) analysis. These proteomic methods have been applied to determine the surface proteins of T. spiralis ML for the detection of trichinellosis in mice [8] and species-speci c ES proteins of T. spiralis adult worms for the diagnosis of human trichinellosis [9]. A previous study of Tattiyapong and colleagues (Ph.D. thesis of Tattiyapong, Department of Helminthology) is focused on 1-DE and immunoblotting of the immune response of pigs against crude extracts of T. spiralis ML and other parasitic infections.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Eluted Antigens from Crude Somatic Antigens of Trichinella Spiralis Muscle Larva by Igg-ELISA, Two-Dimensional Gel Electrophoresis, and Mass Spectrometry

Supcharoengoon

Watthanakulpanich

Limpanont

et al. 2021

Preprint

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Background: Trichinellosis is caused by Trichinella spiralis muscle larvae (ML), which in swine is the main source of transmission. Trichinellosis is detected by enzyme-linked immunosorbent assay (ELISA) using excretory–secretory antigens (ESAg). Preparation of ESAg is difficult, and it produces a low content. Furthermore, the sensitivity and specificity of the test depends on the quality of the antigen that is produced in each batch. Crude somatic antigens (CSAg) of T. spiralis ML at molecular weights (MWs) of 101, 79, and 43 kDa were previously positive for swine trichinellosis sera, with no cross-reaction observed for normal sera, except that 79 and 43 kDa each reacted with one of the coccidiosis and with mixed infections containing trichuriasis and coccidiosis. Therefore, the current study aimed to obtain eluted antigens of T. spiralis ML CSAg, which were analyzed by IgG-ELISA for detecting swine trichinellosis and identification by two-dimensional polyacrylamide gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC MS-MS).Methods: In this study, T. spiralis larvae CSAg at 101, 79, and 43 kDa (TsCSAg-101, TsCSAg-79, and TsCSAg-43, respectively) were eluted from sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The eluted antigens were analyzed by IgG-ELISA for sensitivities and specificities. In addition, three specific antigens were identified by 2-DE and LC MS-MS.Results: Sensitivity of IgG-ELISA using three eluted antigens was persistent at 100%. Specificities were 90.63%, 95.54%, and 97.77% following TsCSAg-101, TsCSAg-79, and TsCSAg-43, respectively. The LC MS-MS results showed that 18/20 spots of the antigens were identified for 11 different proteins. One protein has several isoforms; for example, a serine proteinase and the phosphoenolpyruvate carboxykinase protein.Conclusions: TsCSAg-43 showed the highest specificity compared to the other two eluted antigens, which indicated that these specific proteins (a 45k antigen–trichina [fragment], a DNA topoisomerase 2-alpha, an antigen targeted by protective antibodies, and a conserved hypothetical protein [gi339234223]) should be developed and produced in large volumes in further study.

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The first analysis of Trichinella spiralis and Trichinella britovi adult worm excretory-secretory proteins by two-dimensional electrophoresis coupled with LC-MS/MS

Cited by 9 publications

References 44 publications

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe

Immunoproteomic Analysis of Trichinella britovi Proteins Recognized by IgG Antibodies from Meat Juice of Carnivores Naturally Infected with T. britovi

Proteomic Analysis of Eluted Antigens from Crude Somatic Antigens of Trichinella Spiralis Muscle Larva by Igg-ELISA, Two-Dimensional Gel Electrophoresis, and Mass Spectrometry

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