The Feasibility of industrial production of Spirulina (Arthrospira) in Southern Spain

Please cite this article as: Chua, E.T., Schenk, P.M., A biorefinery for Nannochloropsis: induction, harvesting, and extraction of EPA-rich oil and high-value protein, Bioresource Technology (2017), doi: http://dx.doi.org/10. 1016/ j.biortech.2017.05.124 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractMicroalgae have been studied as biofactories for almost four decades. Yet, even until today, many aspects of microalgae farming and processing are still considered exploratory because of the uniqueness of each microalgal species. Thus, it is important to develop the entire process of microalgae farming: from culturing to harvesting, and down to extracting the desired high-value products. Based on its rapid growth and high oil productivities, Nannochloropsis sp. is of particular interest to many industries for the production of highvalue oil containing omega-3 fatty acids, specifically eicosapentaenoic acid (EPA), but also several other products. This review compares the various techniques for induction, harvesting, and extraction of EPA-rich oil and high-value protein explored by academia and industry to develop a multi-product Nannochloropsis biorefinery. Knowledge gaps and opportunities are discussed for culturing and inducing fatty acid biosynthesis, biomass harvesting, and extracting EPA-rich oil and high-value protein from the biomass of Nannochloropsis sp.

show abstract

“…For example, filtration may be used to harvest filamentous microalgal species like Spirulina platensis (Jiménez et al, 2003).…”

Section: Harvesting Of Nannochloropsis Biomassmentioning

confidence: 99%

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Chua

Schenk

2017

Bioresource Technology

126

View full text Add to dashboard Cite

show abstract

“…The assignments were made according to the literature data [13]. Thus, it was possible to identify: b-carotene (yellow spot, Rf 6 This practice also described how students can prepare their own silica gel TLC plates. In this form of chromatography, the stationary phase is a thin layer of silica which is a type of finely divided silicon dioxide that is deposited on a glass slide.…”

Section: Resultsmentioning

confidence: 99%

“…Spots of crude extract of Spirulina solution (10 %) were applied to the plates using a glass capillary. The separated compounds (Rf [1][2][3][4][5][6] ) were identified by visualizing TLC plates under white light (Fig. 2) or under UV radiation (254 or 365 nm).…”

Section: Analysis and Separation Of The Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

Extraction of nutraceuticals from Spirulina (blue‐green alga): A bioorganic chemistry practice using thin‐layer chromatography

Laguna

Marante

Luna‐Freire

et al. 2015

Biochem Molecular Bio Educ

View full text Add to dashboard Cite

Spirulina is a blue-green alga (cyanobacteria) with high nutritive value. This work provides an innovative and original approach to the consideration of a bioorganic chemistry practice, using Spirulina for the separation of phytochemicals with nutraceutical characteristics via thin-layer chromatography (TLC) plates. The aim is to bring together current research, theory, and practice, and always in accordance with pedagogical ideas. V C 2015 by The International Union of Biochemistry and Molecular Biology, 43(5):366-369, 2015.

show abstract

“…The growth of Microcystis sp. 1023 was monitored by measuring the optical density at the wavelength of 680 nm (nm) (Jiménez et al 2003). The sample was taken by pipette at different time point.…”

Section: Algicidal Effects On Microcystismentioning

confidence: 99%

Effects of intermediate metabolite carboxylic acids of TCA cycle on Microcystis with overproduction of phycocyanin

Bai

Dai

Xia

et al. 2014

Environ Sci Pollut Res

View full text Add to dashboard Cite

Toxic Microcystis species are the main bloomforming cyanobacteria in freshwaters. It is imperative to develop efficient techniques to control these notorious harmful algal blooms (HABs). Here, we present a simple, efficient, and environmentally safe algicidal way to control Microcystis blooms, by using intermediate carboxylic acids from the tricarboxylic acid (TCA) cycle. The citric acid, alphaketoglutaric acid, succinic acid, fumaric acid, and malic acid all exhibited strong algicidal effects, and particularly succinic acid could cause the rapid lysis of Microcystis in a few hours. It is revealed that the Microcystis-lysing activity of succinic acid and other carboxylic acids was due to their strong acidic activity. Interestingly, the acid-lysed Microcystis cells released large amounts of phycocyanin, about 27-fold higher than those of the control. On the other hand, the transcription of mcyA and mcyD of the microcystin biosynthesis operon was not upregulated by addition of alpha-ketoglutaric acid and other carboxylic acids. Consider the environmental safety of intermediate carboxylic acids. We propose that administration of TCA cycle organic acids may not only provide an algicidal method with high efficiency and environmental safety but also serve as an applicable way to produce and extract phycocyanin from cyanobacterial biomass.

show abstract

The Feasibility of industrial production of Spirulina (Arthrospira) in Southern Spain

Cited by 181 publications

References 7 publications

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

A biorefinery for Nannochloropsis: Induction, harvesting, and extraction of EPA-rich oil and high-value protein

Extraction of nutraceuticals from Spirulina (blue‐green alga): A bioorganic chemistry practice using thin‐layer chromatography

Effects of intermediate metabolite carboxylic acids of TCA cycle on Microcystis with overproduction of phycocyanin

Contact Info

Product

Resources

About