The expression of the aryl hydrocarbon receptor in reproductive and neuroendocrine tissues during the estrous cycle in the pig

Jabłońska, Olga; Piasecka, Joanna; Ostrowska, Magdalena; Sobocinska, Natalia; Wasowka, Barbara; Ciereszko, Renata E.

doi:10.1016/j.anireprosci.2011.05.010

Cited by 18 publications

(25 citation statements)

References 45 publications

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“…AHR-knockout mice display a number of defects suggesting that in addition to transducing xenobiotic signals, AHR is involved in the regulation of fundamental physiological processes (Benedict et al, 2003;Nebert et al, 2000). In mammals, the AHR physiological role is supported by the presence of AHR in several tissues, including the reproductive system (Baldridge and Hutz, 2007;Bussmann and Bara nao, 2006;Gregoraszczuk et al, 2001;Gregoraszczuk and Ptak, 2013;Jablonska et al, 2011;Pocar et al, 2004;Schlecht et al, 2004;Wojtowicz et al, 2005). Lee et al (2009) have revealed that both AHRs are expressed in chicken tissues, although only AHR1 mediates TCDD action.…”

Section: Introductionmentioning

confidence: 93%

Expression of aryl hydrocarbon receptor 1 (AHR1), AHR1 nuclear translocator 1 (ARNT1) and CYP1 family monooxygenase mRNAs and their activity in chicken ovarian follicles following in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

et al. 2015

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Section: Introductionmentioning

confidence: 93%

Expression of aryl hydrocarbon receptor 1 (AHR1), AHR1 nuclear translocator 1 (ARNT1) and CYP1 family monooxygenase mRNAs and their activity in chicken ovarian follicles following in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

et al. 2015

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“…Based on the identified partial AHR mRNA sequence, the specific pair of primers was designed and used in real‐time PCR in both this and our previous study (Jablonska et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…Quantitative real‐time PCR was used to determine the mRNA expression of the AHR in ovarian cells (Jablonska et al. ). Specific primers and probes for AHR and beta‐actin ( ACTB ; a housekeeping gene) were designed and synthesized by Applied Biosystems (Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The cell lysate preparation from isolated ovarian cells (approximately 20 × 10 6 cells) as well as Western blot analysis was performed as described by Jablonska et al. (). Cell lysates containing 20 μg of protein were resolved by SDS‐PAGE (8% polyacrylamide gel) and transferred to nitrocellulose membranes (Schleicher & Schuell BioScience GmbH, Dassel, Germany) using the Hoefer SemiPhor apparatus (Pharmacia Biotech, Golden Valley, MN, USA) for semidry electroblotting (0.8 mA/cm 2 gel, 115 min).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we have demonstrated AHR mRNA and protein expression in the whole ovary, oviduct and uterus during the follicular and luteal phases of the porcine oestrous cycle (Jablonska et al. ). However, data concerning the AHR expression in different ovarian cells of the pig are scarce and unsatisfactory.…”

Section: Introductionmentioning

confidence: 99%

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The Expression of Aryl Hydrocarbon Receptor in Porcine Ovarian Cells

Jabłońska

Ciereszko

2013

Reprod Domestic Animals

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The aryl hydrocarbon receptor (AHR) is an intracellular transcription factor best known for mediating the toxicity of dioxins. The AHR is phylogenetically highly conserved among invertebrates and vertebrates and may play a significant role in the regulation of physiological processes including female reproduction. This study was performed to determine the partial nucleotide sequence of porcine AHR and to evaluate the AHR mRNA (real-time PCR) and AHR protein (Western blot) expression in granulosa and theca interna cells harvested from medium (3-6 mm) and pre-ovulatory (≥8 mm) follicles as well as in luteal cells obtained from corpora lutea collected during the mid-luteal phase (days: 8-10) of the porcine oestrous cycle. In the study, the partial nucleotide sequence of porcine AHR containing 1021 bp (GenBank accession no: HM488957.1) was determined. The AHR transcript and protein were found in all ovarian cells obtained during both phases of the porcine oestrous cycle. The highest AHR transcript level was detected in theca interna cells isolated from pre-ovulatory follicles as well as in luteal cells. Higher AHR protein expression was found in granulosa cells isolated from pre-ovulatory follicles in comparison with all remaining cell types. The presence of AHR in the examined ovarian cells may account for their sensitivity to some environmental pollutants. Moreover, the differences found in AHR mRNA expression between granulosa and theca cells as well as between cells originating from follicles of different size suggest the involvement of AHR in the modulation of reproductive processes in the porcine ovary.

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Changes in the expression of genes involved in the ovarian function of rats caused by daily exposure to 3-methylcholanthrene and their prevention by α-naphthoflavone

et al. 2017

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Daily exposure to low doses of 3-methylcholanthrene (3MC) during the pubertal period in rats disrupts both follicular growth and ovulation. Thus, to provide new insights into the toxicity mechanism of 3MC in the ovary, here we investigated the effect of daily exposure to 3MC on selected ovarian genes, the role of the aryl hydrocarbon receptor (AhR) and the level of epigenetic remodeling of histone post-transcriptional modifications. Immature rats were daily injected with 3MC (0.1 or 1 mg/kg) and mRNA expression of genes involved in different ovarian processes were evaluated. Of the 29 genes studied, 18 were up-regulated, five were down-regulated and six were not altered. To assess whether AhR was involved in these changes, we used the chromatin immunoprecipitation assay. 3MC increased AhR binding to promoter regions of genes involved in Notch signaling (Hes1, Jag1), activation of primordial follicles (Cdk2), cell adhesion (Icam1), stress and tumor progression (Dnajb6), apoptosis (Bax, Caspase-9) and expression of growth and transcription factors (Igf2, Sp1). Studying the trimethylation and acetylation of histone 3 (H3K4me3 and H3K9Ac, respectively) of these genes, we found that 3MC increased H3K4me3 in Cyp1a1, Jag1, Dnajb6, Igf2, Notch2, Adamts1, Bax and Caspase-9, and H3K9Ac in Cyp1a1, Jag1, Cdk2, Dnajb6, Igf2, Icam1, and Sp1. Co-treatment with α-naphthoflavone (αNF), a specific antagonist of AhR, prevented almost every 3MC-induced changes. Despite the low dose used in these experiments, daily exposure to 3MC induced changes in both gene expression and epigenomic remodeling, which may lead to premature ovarian failure.

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The expression of the aryl hydrocarbon receptor in reproductive and neuroendocrine tissues during the estrous cycle in the pig

Cited by 18 publications

References 45 publications

Expression of aryl hydrocarbon receptor 1 (AHR1), AHR1 nuclear translocator 1 (ARNT1) and CYP1 family monooxygenase mRNAs and their activity in chicken ovarian follicles following in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

Expression of aryl hydrocarbon receptor 1 (AHR1), AHR1 nuclear translocator 1 (ARNT1) and CYP1 family monooxygenase mRNAs and their activity in chicken ovarian follicles following in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

The Expression of Aryl Hydrocarbon Receptor in Porcine Ovarian Cells

Changes in the expression of genes involved in the ovarian function of rats caused by daily exposure to 3-methylcholanthrene and their prevention by α-naphthoflavone

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