The expression of miRNA-221 and miRNA-222 in gliomas patients and their prognosis

Li, Xue; Wang, Yi; Yue, Shuyuan; Zhang, Jianning

doi:10.1007/s10072-016-2710-y

Cited by 31 publications

(23 citation statements)

References 31 publications

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“…Glioma is an aggressive tumor and the average survival time in patients with high-grade glioma is not more than one year. [27][28][29][30] Sev was reported to be involved in the regulation of glioma progression 4,31 and our data showed that Sev inhibited glioma progression in vitro and tumor growth in vivo. However, the current studies were mostly focused on the regulatory mechanisms of miRNAs and long noncoding RNAs in Sev-mediated human diseases.…”

Section: Discussionsupporting

confidence: 59%

<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

Xia

Guan³

et al. 2020

CMAR

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Background: Glioma is a common malignant tumor worldwide. Sevoflurane (Sev) has been reported to inhibit the metastasis of glioma cells, but the underlying molecular mechanism needs further exploration. Methods: Cell Counting Kit-8 (CCK8) assay was used to check cell viability. Flow cytometry assay was hired to check cell apoptosis. The protein levels of B-cell lymphoma-2 (Bcl-2), BCL2-Associated X (Bax), hexokinase 2 (HK2) and magnesium transporter 1 (MAGT1) in samples were measured by Western blot. The abilities of cell migration and invasion were estimated by transwell assay. Glucose colorimetric assay kit and lactate colorimetric assay kit were used to check glucose consumption and lactate production, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the levels of circular RNA (circRNA) circ_0002755 (also known as the circRNA1656) and microRNA (miR)-628-5p in samples. The interaction between miR-628-5p and circ_0002755 or MAGT1 was predicated by starBase, which was verified by the dual-luciferase reporter assay. Xenograft tumor model was established to explore the biological role of circ_0002755 in vivo. Results: Sev inhibited cell viability, migration, invasion and promoted cell apoptosis, and also reduced glucose consumption and lactate production. Circ_0002755 was significantly upregulated in glioma tissues and cells, while its level was notably declined under Sev treatment. Besides, overexpression of circ_0002755 overturned Sev-mediated inhibitory effect on glioma progression. Further research indicated that circ_0002755 targeted miR-628-5p, and miR-628-5p targeted MAGT1, and Sev modulated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis. Moreover, Sev hindered tumor growth in vivo. Conclusion: Sev mediated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis.

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Section: Discussionsupporting

confidence: 59%

<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

Xia

Guan³

et al. 2020

CMAR

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“…A large number of studies have reported that certain miRNA are involved in the regulation of tumor progression, and may have tumor-promoting or tumor-suppressive roles with regard (25)(26)(27). miRNAs may be used as prognostic indicators and therapeutic targets in gliomas (28,29). In the present study, comprehensive miRNA profiling of high-grade vs. low-grade gliomas was performed by microarray analysis.…”

Section: Discussionmentioning

confidence: 95%

Downregulation of microRNA‑374a predicts poor prognosis in human glioma

et al. 2019

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Certain microRNAs (miRNAs/miRs) may be used as prognostic biomarkers in various types of cancer. The purpose of the present study was to identify miRNAs that were abnormally expressed in glioma of different grades, and to evaluate their clinical implications in patients with glioma. The differentially expressed miRNAs were evaluated from the expression profiles of six glioma tissues (three low-grade and three high-grade gliomas) determined using a microarray platform. Reverse transcription-quantitative polymerase chain reaction analysis was used to further verify the aberrant expression of the candidate miRNA in a set of 42 patients and 5 healthy controls. The miRNA target genes were predicted and the protein-protein interaction network was generated; furthermore, functional enrichment analysis of the target genes in Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was performed. Kaplan-Meier curves and Log-rank analysis, as well as multivariate Cox regression analysis were performed to assess the association of the candidate miRNA with patient survival. A total of 15 differentially expressed miRNAs, including 13 downregulated and 2 upregulated miRNAs, were identified by comparison of low-grade and high-grade glioma tissues. The miR-374a expression of high-grade gliomas was significantly lower than that of low-grade gliomas (fold change, -4.43; P=0.027). The expression levels of miR-374a gradually decreased with the increase of the pathological grade of glioma. Pearson's Chi-square test was used to determine the association of miR-374a expression with several clinicopathological factors. Furthermore, low expression of miR-374a was determined to be an independent prognostic marker and that it was significantly associated with overall survival (P=0.0213). GO and KEGG pathway analysis revealed that the target genes of miR-374a may be involved in the regulation of the RNA polymerase II promoter and mTOR signaling pathway. The four hub genes (CCND1, SP1, CDK4, CDK6) were also identified by PPI network analysis. In conclusion, the present study indicated that miR-374a may be used as a promising prognostic biomarker for the screening of high-risk populations and for the assessment of the prognosis of patients with glioma.

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“…For instance, Nie S. et al demonstrated that miR-495 induced the change of metabolism in glioma cells by increasingly Glut1 expression, resulting in the increase of glucose-taking and glioma developing [14]. MiRNA-221 and miRNA-222 were found to be highly expressed in glioma and the expression of which was closely related to survival rates of glioma cells [15]. Herein we provided an insight into the relationship of glioma and another miRNA, miR-338-3p, which have been discovered essential in the development of non-small-cell lung carcinoma, ovarian epithelial carcinoma, and gastric cancer [16][17][18].…”

Section: Micrornas (Mirnas) Another Family Of Non-codingmentioning

confidence: 99%

Circular RNA SMO sponges miR-338-3p to promote the growth of glioma by enhancing the expression of SMO

Zhang

Zhou

Wang

et al. 2019

Aging

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Glioma is one of the most common tumors in the brain and complete cure still a challenge. The present research aimed to investigate the molecular mechanism of circular RNA SMO (circSMO742) in glioma, via targeting miR-338-3p and regulating SMO expression. QRT-PCR was utilized to examine the expression profiles of circSMO742 and microRNA-338-3p (miR-338-3p) in glioma. SMO protein in glioma was tested via western blot. RNA pulldown assay and dual luciferase reporter assays were used to explore the targeting correlation between RNAs. MTT assay, transwell assays and flow cytometry were used to investigate cell proliferation, migration and invasion, and apoptosis, respectively. Tumor xenograft was done to ascertain the effect of circSMO742 knocking down on tumor growth. CircSMO742 and SMO were highly expressed in glioma tissues, while miR-338-3p expression was reduced. CircSMO742 together with SMO could promote cells proliferation, migration and invasion while inhibit cells apoptosis, whereas miR-338-3p showed negative impacts on the cell activity. Knocking down of circSMO742 suppressed glioma growing in vivo. CircSMO742 promoted glioma growth by sponging miR-338-3p to regulate SMO expression. Our research revealed a new molecular mechanism of glioma growth and provide a fresh perspective on circRNAs in glioma progression.

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The expression of miRNA-221 and miRNA-222 in gliomas patients and their prognosis

Cited by 31 publications

References 31 publications

<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

Downregulation of microRNA‑374a predicts poor prognosis in human glioma

Circular RNA SMO sponges miR-338-3p to promote the growth of glioma by enhancing the expression of SMO

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