The Expression of Mannose Receptors in Skin Fibroblast and Their Involvement in Leishmania (L.) amazonensis Invasion

Hespanhol, Renata C.; Soeiro, Maria de Nazaré Correia; Meuser, M. B.; Meirelles, Maria de Nazareth Leal de; Cortê-Real, Suzana

doi:10.1177/002215540505300105

Cited by 28 publications

(24 citation statements)

References 67 publications

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“…Leishmania spp. differentiation, maturation and replication in vitro has been achieved in cultures of human macrophage cells [5,6]; J774 (murine) macrophages [7,8]; fibroblast cell lines [9,10], epithelial cells [11], dendritic cells [12,13], neutrophil granulocytes [14]; Aedes albopictus cells [15], and Aedes aegypti cells [16,17], however, there are few studies about the life cycle of Leishmania promastigotes in phlebotomine sandflies which use insect cell lines [18,19]. …”

Section: Resultsmentioning

confidence: 99%

Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae): a novel model to assay Leishmania spp. and vector interaction

Côrtes¹,

Silva²,

Pereira³

et al. 2011

Parasites Vectors

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BackgroundLeishmania (Vianna) braziliensis, Leishmania (Leishmania) amazonensis and Leishmania (Leishmania) chagasi are important parasites in the scenario of leishmaniasis in Brazil. During the life cycle of these parasites, the promastigote forms adhere to the midgut epithelial microvillii of phlebotomine insects to avoid being secreted along with digestive products. Lulo cells are a potential model that will help to understand the features of this adhesion phenomenon. Here, we analyze the interaction between Leishmania spp. promastigotes and Lulo cells in vitro, specifically focusing on adhesion events occurring between three Leishmania species and this cell line.MethodsConfluent monolayers of Lulo cells were incubated with promastigotes and adhesion was assessed using both light microscopy and scanning electron microscopy.FindingsThe results indicate that species from the subgenera Leishmania and Viannia have great potential to adhere to Lulo cells. The highest adherence rate was observed for L. (L.) chagasi after 24 h of incubation with Lulo cells (27.3 ± 1.8% of cells with adhered promastigotes), followed by L. (L.) amazonensis (16.0 ± 0.7%) and L. (V.) braziliensis (3.0 ± 0.7%), both after 48 h. In the ultrastructural analysis, promastigote adherence was also assessed by scanning electron microscopy, showing that, for parasites from both subgenera, adhesion occurs by both the body and the flagellum. The interaction of Lulo cells with Leishmania (L.) chagasi showed the participation of cytoplasmic projections from the former closely associating the parasites with the cells.ConclusionsWe present evidence that Lulo cells can be useful in studies of insect-parasite interactions for Leishmania species.

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Section: Resultsmentioning

confidence: 99%

Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae): a novel model to assay Leishmania spp. and vector interaction

Côrtes¹,

Silva²,

Pereira³

et al. 2011

Parasites Vectors

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“…In view of the expression in resident, apparently non-activated cells, MR positivity in the tissue surrounding subcutaneous implants should also be interpreted with caution when MR is used as a marker of M2 activation. It is also apparent that MR expression is not restricted to macrophages [Hespanhol et al, 2005]. Triple or double labelling using combinations of MR, CD68 and CD163 antibodies would have been valuable in assessing the contribution of both CD68+ and CD163+ macrophage subsets to the M2 subset as identified by MR labelling, but we were unable to achieve successful multiple labelling on our paraffin-embedded samples due to the particular antigen retrieval requirements for each antibody.…”

Section: Discussionmentioning

confidence: 96%

Macrophage Phenotype in Response to Implanted Synthetic Scaffolds: An Immunohistochemical Study in the Rat

Palmer

Abberton

Mitchell

et al. 2014

Cells Tissues Organs

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Macrophages predominate among the cells that directly interact with biomaterials and are key orchestrators of host-biomaterial interactions. However, the macrophage response to synthetic scaffolds in particular has not been well studied. The aim of this study was therefore to characterise the macrophage response to several synthetic scaffolds in the rat using immunohistological techniques for a panel of markers of macrophage subclass or activation, including ED1 (CD68), ED2 (CD163), CD80, mannose receptor and inducible nitric oxide synthase (iNOS). Materials were implanted subcutaneously and collected after 6-8 weeks during the chronic phase of the host response. Unmodified polycaprolactone scaffolds uniquely demonstrated a total lack of both macrophage adherence to surfaces and a wider foreign body response compared to scaffolds composed of poly(lactic-co-glycolic acid) (PLGA) and polyurethanes (PURs), with those macrophages present having a clear M2 (MR+, CD80-, iNOS-) phenotype. PLGA scaffolds displayed an M1-dominant (CD80+, iNOS+, MR-) response with substantial foreign body giant cell (FBGC) formation, whilst PUR scaffold FBGCs had a more mixed M1 (CD80+, iNOS+) and M2 (MR+) phenotype. The study also identified that the use of the ED1 antibody in the rat as a pan-macrophage marker is problematic as there is a separate and substantial ED2-positive macrophage population that it does not label, both in response to biomaterials and in normal tissues. The biomaterial-dependent nature of activation for both macrophages and FBGCs was confirmed, and nuanced M1/M2 phenotypes were described.

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“…The glycosylation of the anchoring region might have a function similar to that of PTP1 glycosylation in facilitating the attachment of the spore body to the host cell membrane during invasion. Various parasites have been shown to use carbohydrate receptors for adhesion and invasion of host cells (2,15,35).…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate Moieties of Microsporidian Polar Tube Proteins Are Targeted by Immunoglobulin G in Immunocompetent Individuals

et al. 2005

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Microsporidia of the Encephalitozoon species are frequently found as opportunistic pathogens of immunocompromised patients, but very little is known about the prevalence and significance of Encephalitozoon infection in immunocompetent individuals. It was reported previously that 8% of Dutch blood donors and 5% of pregnant French women had an immunoglobulin G (IgG) immune response against specific organelles of Encephalitozoon intestinalis. These organelles, the so-called polar tube and anchoring disk, are used to penetrate membranes of host cells during infection. The unexpectedly high percentage of immunocompetent individuals with IgG against these organelles suggested that infection of humans with microsporidia might be more common than previously recognized. In the present study, we analyzed this anti-Encephalitozoon IgG response by using indirect immunofluorescence, Western blotting, two-dimensional gel electrophoresis, and chemical deglycosylation. Our results show that the antibody response is directed against the posttranslational carbohydrate modification of the major polar tube protein (polar tube protein 1) and carbohydrate moieties of proteins in the anchoring region of the polar tube of Encephalitozoon. In addition, the antibodies were found to decrease the infectivity of E. intestinalis in vitro. The significance and possible origin of these prevalent antibodies are discussed.

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The Expression of Mannose Receptors in Skin Fibroblast and Their Involvement in Leishmania (L.) amazonensis Invasion

Cited by 28 publications

References 67 publications

Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae): a novel model to assay Leishmania spp. and vector interaction

Lulo cell line derived from Lutzomyia longipalpis (Diptera: Psychodidae): a novel model to assay Leishmania spp. and vector interaction

Macrophage Phenotype in Response to Implanted Synthetic Scaffolds: An Immunohistochemical Study in the Rat

Carbohydrate Moieties of Microsporidian Polar Tube Proteins Are Targeted by Immunoglobulin G in Immunocompetent Individuals

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