2017
DOI: 10.18632/oncotarget.21514
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Abstract: Hydrogen sulfide (H2S), generated in the osteoblasts predominantly via cystathionine-γ-lyase (CSE), is bone protective. Previous studies suggested that the onset of bone loss due to estrogen deficiency is associated to decreased levels of H2S and blunted gene expression of CSE. However, there are still a lot of unknowns on how H2S levels influence bone cells function. The present study aims to explore the mechanisms by which estrogen may regulate CSE expression, in particular the role of estrogen receptor alph… Show more

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Cited by 18 publications
(11 citation statements)
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“…Chromatin immunoprecipitation (ChIP) was performed by using Magna ChIP Protein A+G magnetic beads (Merck Millipore, Billerica, MA, USA) according to the manufacturer’s instructions and as previously described [22,23]. Briefly, IVD progenitors were cross-linked with 1% formaldehyde at 37 °C for 10 min.…”
Section: Methodsmentioning
confidence: 99%
“…Chromatin immunoprecipitation (ChIP) was performed by using Magna ChIP Protein A+G magnetic beads (Merck Millipore, Billerica, MA, USA) according to the manufacturer’s instructions and as previously described [22,23]. Briefly, IVD progenitors were cross-linked with 1% formaldehyde at 37 °C for 10 min.…”
Section: Methodsmentioning
confidence: 99%
“…Sulfide biosynthetic enzymes are subject to transcriptional and post-translational regulation. Oxidative stress stimulates ATF4-and Nrf2-dependent CSE transcription [99,100], and estrogen (E 2 ) promotes CSE activity in human osteoblasts and mouse liver and vasculature [101,102]. Multiple allosteric mechanisms regulate CBS activity.…”
Section: Sulfidementioning
confidence: 99%
“…Briefly, bone fragments were placed in sterile PBS at 4°C and dissected within 16 h after removal. Bone chips were minced into smaller pieces as previously reported ( 17 ), washed twice with PBS, plated in T-25 culture flasks (Sarstedt, Inc.) and cultured in high-glucose DMEM/Ham's F12 (1:1) supplemented with 10% FCS, 1 mM L-glutamine and antibiotics [(penicillin (100 µ g/ml) and streptomycin, (10 µ g/ml)]. From each patient, a primary cell culture was obtained.…”
Section: Methodsmentioning
confidence: 99%