The expression characteristics of mt-ND2 gene in chicken

Abstract

Subunit 2 of NADH dehydrogenase (ND2) is encoded by the mt-ND2 gene and plays a critical role in controlling the production of the mitochondrial reactive oxygen species. Our study focused on exploring the mt-ND2 tissue expression patterns and the effects of energy restriction and dietary fat (linseed oil, corn oil, sesame oil or lard) level (2.5% and 5%) on its expression in chicken. The results showed that mt-ND2 gene was expressed in the 15 tissues of hybrid chickens with the highest level in heart and lowest level in pancreas tissue; 30% energy restriction did not significantly affect mt-ND2 mRNA level in chicken liver tissue. Both the mt-ND2 mRNA levels in chicken pectoralis (p < 0.05) and hepatic tissues (p < 0.05) at 42 d-old were affected by the type of dietary fats in 5% level, while not in abdominal fat tissues. The expression of mt-ND2 in hepatic tissues was down-regulated with chicken age (p < 0.01). The interactive effect of dietary fat types with chicken age (p < 0.05) was significant on mt-ND2 mRNA level. The study demonstrated that mt-ND2 gene was extensively expressed in tissues, and the expression was affected by dietary fat types and chicken age.

Multi-carbohydrase enzymes improve feed energy in broiler diets containing standard or low crude protein

“…This suggests that higher expression of muscle avPGC-1A is an indicator of slower growth. ND2 is another gene that controls the production of the mitochondrial ROS and is negatively affected by high dietary fat content and bird aging ( Zhang et al., 2016 ). The results from this study demonstrated that the level of muscle ND2 mRNA expression was higher in the LCP- than the SCP-fed birds.…”

The correlated expression of immune and energy metabolism related genes in the response to Salmonella enterica serovar Enteritidis inoculation in chicken

“…Mitochondrial complex V consists of 19 genes in human. The function of genes involved in complex I and V has been reported, such as mt-ND2 [ 19 ], ATP5E [ 20 ], and ATP5G3 [ 21 , 22 ]. Subunit 2 of NADH dehydrogenase (ND2) is one of genes comprising of mitochondrial complex I [ 23 ], which plays a critical role in controlling the production of the mitochondrial reactive oxygen species in chicken [ 24 ].…”

“…Subunit 2 of NADH dehydrogenase (ND2) is one of genes comprising of mitochondrial complex I [ 23 ], which plays a critical role in controlling the production of the mitochondrial reactive oxygen species in chicken [ 24 ]. Mt-ND2 gene was extensively expressed in tissues, and the expression was affected by dietary fat types and chicken age [ 19 ]. The altered expression of mt-ND2 is critical to multiple disease in human [ 25 , 26 ].…”

Differential gene expression profiling reveals potential biomarkers and pharmacological compounds against SARS-CoV-2: insights from machine learning and bioinformatics approaches

“…Jha et al [24]discovered 338 DEGs in the RNA-seq dataset of lung epithelial cells infected with SARS-CoV-2, including 92 increased and 246 decreased genes across the datasets. In this study, top abundant DEGs such as genes encoding for ribosomal protein (RPL4), controlling the production of the mitochondrial reactive oxygen species (MT-ND2) [25], modulating cell proliferation and differentiation (SCD5) [26], mitochondrial deficiencies, and associated disorders (MT-CYB) [27], epithelial marker ezrin (EZR) associated with cell surface structure adhesion, migration, and organization of the SARS-CoV-2 [28] were found to be co-expressed in the nasopharyngeal epithelial cells of COVID-19 patients and recovered humans (Fig. 2C).…”