The expression and localization of leptin and its receptor in goat ovarian follicles

Batista, André Mariano; Silva, D.M.F.; Rêgo, Moacyr Jesus Barreto de Melo; Silva, F.L.M.; Silva, Ellen Cordeiro Bento da; Beltrão, Eduardo Isidoro Carneiro; Filho, Manoel Adrião Gomes; Wischral, Áurea; Guerra, Maria Madalena Pessoa

doi:10.1016/j.anireprosci.2013.08.007

Cited by 33 publications

(33 citation statements)

References 25 publications

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“…Recently, we have demonstrated that leptin and its receptor are expressed at both the mRNA and protein levels in goat ovarian follicles (Batista et al. ), suggesting that leptin is a local factor in the control of ovarian function in this species. This information prompted us to hypothesize that administration of leptin during superovulation might influence superovulatory response and improve the embryo production in goats.…”

Section: Introductionmentioning

confidence: 97%

Effect of Leptin on In Vivo Goat Embryo Production

Batista

Gomes

Carvalho

et al. 2014

Reprod Domestic Animals

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The aim of this study was to evaluate the effect of leptin administration during superovulation on in vivo goat embryo production. Ten mature does were superovulated with 133 mg follicle-stimulating hormone (FSH) i.m. in six descending doses at 12-h intervals. The goats received 4.8 μg/kg human recombinant leptin s.c. (leptin group, n = 5) or phosphate-buffered saline (PBS) (control group, n = 5) with the first and second FSH doses. The does were mated and subjected to embryo collection by transcervical technique 6 days later. The total number of cells per embryo and the number of cells with fragmented DNA were assessed in selected blastocysts by combining Hoechst 33342 and terminal dUTP nick-end labelling (TUNEL) staining. Plasma concentrations of oestradiol (E2 ) and progesterone (P4 ) were determined by electrochemiluminescence from the day of FSH treatment, on the day of superovulatory oestrus and on the day before embryo collection. Compared with the control group, the does that received leptin had a higher number of transferable embryos (p < 0.005), fewer embryos classified as degenerated (p < 0.001) and fewer TUNEL-positive cells/blastocyst (p < 0.001). The number of transferable embryos was positively correlated with E2 concentrations on day of oestrus (r = 0.562; p < 0.01) and P4 concentrations on the day of embryo collection (r = 0.912; p < 0.001). We concluded that in vivo leptin administration during FSH treatment improved embryo quality and affected ovarian steroidogenesis in superovulated goats.

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Section: Introductionmentioning

confidence: 97%

Effect of Leptin on In Vivo Goat Embryo Production

Batista

Gomes

Carvalho

et al. 2014

Reprod Domestic Animals

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“…The leptin (LEP) gene encodes a 16-kDa adipokine that has pleiotropic effects on the modulation of feed intake, fat deposition, body weight, immune function, and reproduction (Friedman and Halaas, 1998;Chan et al, 2006;Batista et al, 2013). This adipocyte-derived hormone, leptin, is primarily synthesized by white adipose tissue and acts on the hypothalamus.…”

Section: Introductionmentioning

confidence: 99%

The novel SNPs of leptin gene and their associations with growth traits in Chinese Nanjiang Yellow goat

Wang

Zhang

Niu

et al. 2015

Gene

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“…These important observations have emphasized the possible link between leptin and the reproductive activity of animals . Those authors also showed an indirect link between leptin, nutritional status and reproductive activity, while other researchers reported that leptin acts directly and is produced in different organs of the reproductive apparatus where its specific receptor is also found . Among various studies, some have reported the presence of leptin and its receptor in exocrine glands of the reproductive apparatus.…”

Section: Introductionmentioning

confidence: 95%