The evaluation of bcl-2 expression as a prognostic marker in early stage laryngeal cancer

Gurlek, Umit; Abakay, Candan Demiröz; Özkan, Lütfi; Saraydaroğlu, Özlem; Kurt, Meral; Çetintaş, Sibel Kahraman

doi:10.1177/030089161309900607

Cited by 4 publications

(3 citation statements)

References 53 publications

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“…Previous studies have reported that BCL2 is involved in the pathogenesis of laryngeal cancer (10)(11)(12). In the present study, it was observed that transfection of M4e cells with lenti-miR-34c significantly inhibited cell viability and BCL-2 protein expression, and increased the percentage of cells undergoing apoptosis.…”

Section: Discussionsupporting

confidence: 61%

“…Furthermore, miR-34a/c expression was observed to be downregulated in human laryngeal squamous cell carcinoma tissues, and upregulation of miR-34a/c in Hep-2 cells by transfection significantly decreased cell proliferation and migratory ability in vitro (2). It was previously reported that B cell lymphoma 2 (BCL2), a central genetic apoptotic regulator (9), which is crucial for cell apoptosis, migration and invasion (3), is involved in the pathogenesis of laryngeal cancer (10)(11)(12). In addition, research into other diseases has reported on the association between miR-34c and BCL2 (13)(14)(15); there is an inverse association between levels of miR-34c and expression of BCL2.…”

Section: Introductionmentioning

confidence: 99%

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MiR-34c induces apoptosis and inhibits the viability of M4e cells by targeting BCL2

Zhang

Zheng

2017

Oncol Lett

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Abstract. The present study aimed to investigate microRNA (miR/miRNA)-34c expression and the association of miR-34c with B cell lymphoma 2 (BCL2) in M4e laryngeal carcinoma cell line. M4e laryngeal carcinoma cells were cultured and transfected with lenti-miR-34c or scramble miRNA for 72 h. Cell viability and the percentage of cells undergoing apoptosis of transfected cells were detected using MTT and Annexin V/allophycocyanin and propidium iodide assays, respectively. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis were performed to determine BCL2 mRNA and protein expression in transfected M4e cells. In addition, luciferase reporter assay was performed to identify whether BCL2 is a direct target of miR-34c. Transfection of lenti-miR-34c was able to significantly inhibit cell viability (P<0.01), increase the percentage of cells undergoing apoptosis (P<0.001) and downregulate BCL2 protein expression (P<0.01) in M4e cells. RT-qPCR data revealed that lenti-miR-34c transfection did not affect BCL2 mRNA expression. However, data from the luciferase reporter assay revealed that transfection with miR-34c negative control decreased luciferase activity in M4e cells co-transfected with pGL3-BCL2-MUT plasmid, compared with miR-34c inhibitor (P<0.01). Collectively, the results from the present study provided evidence that miR-34c may be involved in the pathogenesis of laryngeal cancer, and BCL2 may be negatively regulated by miR-34c in M4e cells.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

MiR-34c induces apoptosis and inhibits the viability of M4e cells by targeting BCL2

Zhang

Zheng

2017

Oncol Lett

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“…The bcl2 gen encodes for a mitochondrial protein which prevents apoptosis and prolongs cell survival, thus antagonizing the effects of the p53 protein [37,38]. This suggest the possibility that increased expression of bcl-2 in mammary tumors from smokers' women determine a worse prognosis by preventing apoptosis [39] or be accompanied by a better prognosis as have been shown in these malignant neoplasms [40].…”

Section: Discussionmentioning

confidence: 96%

Smoking Habit and Clinico-Biological Parameters of Breast Cancer

Ruibal¹,

Aguiar²,

Menéndez³

et al. 2017

J Tob Stimulated Dis

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Introduction: The aim of this study was to analyse the possible associations between smoking habit and some clinico-biological parameters of breast infi ltrating ductal carcinomas (IDCs). Material and Methods:The study group included included 291 females with IDC who had undergone no prior treatment. Out of them, 48 were current smoking, 11 quit smoking and 232 never smoking. All were studied at the same Breast Cancer Unit. Age, tumor size, axillary lymph node involvement (N), distant metastasis (M) and histological grade (HG), as well as the immunohistochemical expression of estrogen receptor (ER), progesterone receptor (PgR), Ki67, p53, bcl2 and androgen receptor (AR) were analyzed Also, we dose the serum levels of CEA and CA15.3. We can follow up 276 patients during a period of time which ranged between 8 and 240 months (83,3+/-51,8; median 84 months). Results:IDCs of smoking habit subgroup had lower age than nonsmoking habit subgroup and were more frequently bcl-2+ (p 0,083). There were not differences in the other clinico-biological parameters as well as in the follow-up considering recurrences and deaths due to the tumor. Likewise, age of ex-smoking habit subgroup was similar to that observed in active smoking habit subgroup, and lower than that observed in nonsmoking habit subgroup (p: 0,003). Smokers women showed more frequently history of contraceptives intake (26,4% vs 7,8% ; p<0,001). When we considered the molecular subtypes of IDCs, we not observed any statistically difference; nevertheless, when we analyzed current and quit versus never smoking patients, , we noted than luminal B subtype was less frequent in those (p: 0,068) and the tumors were more frequent bcl-2 positive (p:0,048) than never smoking subgroup. Conclusion:Our results led us to the following: 1) Women with IDCs and smoking habit had lower age and the tumors were more frequently bcl2 positive than never smoking habit; 2) age of ex smoking habit subgroup was similar to that observed in active smoking habit subgroup, and lower than that observed in nonsmoking habit subgroup ; 3) in smoking/ex-smoking women luminal B molecular subtype was less frequent than in nonsmoking women. The smoking habit has been subject of intense study since its modifi able factor character. We know that there is a higher risk of benign tumors, preferably fi broadenomas, in current smokers, but without statistically signifi cant [2,3] and that tobacco smoke has been implicated in various human disease conditions and the International Agency for Research on Cancer identifi ed tobacco smoking as the cause of cancer at more organ sites that any other human carcinogen [4]. In relation to breast cancer, some groups have denied such a relationship [5][6][7][8][9]; others have shown a positive discreet relationship especially in premenopausal women [10][11][12][13], others an inverse relationship [14,15], and fi nally some suggest an increased risk associated with time, quantity and age of onset of the habit [12][13][14][15][16]. Xue et al. [17] observed that...

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Risk factors for radiation failure in early-stage glottic carcinoma: A systematic review and meta-analysis

et al. 2016

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The evaluation of bcl-2 expression as a prognostic marker in early stage laryngeal cancer

Abstract: No correlation was observed between bcl-2 expression and local control or overall survival. Whereas hemoglobin level, age and existence of a recurrence had a prognostic impact on overall survival, patient age and smoking status influenced local control rates.

Cited by 4 publications

References 53 publications

MiR-34c induces apoptosis and inhibits the viability of M4e cells by targeting BCL2

MiR-34c induces apoptosis and inhibits the viability of M4e cells by targeting BCL2

Smoking Habit and Clinico-Biological Parameters of Breast Cancer

Risk factors for radiation failure in early-stage glottic carcinoma: A systematic review and meta-analysis

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