volume 273, issue 1-2, P213-219 2004
DOI: 10.1016/j.ijpharm.2004.01.006
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Abstract: The major hydrolytic degradation pathways of glucagon under acidic conditions are cleavage at Asp-9, Asp-15, and Asp-21, and deamidation at Gln-3, Gln-20, Gln-24, and Asn-28. The rate constants for these pathways were determined in the pH range 1-2.4 at 60 degrees C by kinetic data analysis of substrate and degradation product concentration-time profiles. Deamidation kinetics were determined using penta-peptide fragments of glucagon containing the labile amide residue. The accurate determination of the cleavag…

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