The Essential Role of 17-Octadecynoic Acid in the Pathogenesis of Periapical Abscesses

Altaie, Alaa Muayad; Mohammad, Mohammad G.; Madkour, Mohamed; Shakartalla, Sarra B.; Jayakumar, Manju Nidagodu; Rani, Aghila K.G; Halwani, Rabih; Samsudin, Abdul Rani; Hamoudi, Rifat; Soliman, Sameh S. M.

doi:10.1016/j.joen.2022.12.002

Cited by 4 publications

(1 citation statement)

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“…Analysis and characterization of the markers were performed using BD FlowJo software (RRID:SCR_014422, V 10.8.0). The gating strategies was performed as previously described 68 .…”

Section: Methodsmentioning

confidence: 99%

Molecular pathogenicity of 1-nonadecene and l-lactic acid, unique metabolites in radicular cysts and periapical granulomas

Altaie

Mohammad

Madkour

et al. 2023

Sci Rep

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Recently, 1-nonadecene and l-lactic acid were identified as unique metabolites in radicular cysts and periapical granuloma, respectively. However, the biological roles of these metabolites were unknown. Therefore, we aimed to investigate the inflammatory and mesenchymal-epithelial transition (MET) effects of 1-nonadecene, and the inflammatory and collagen precipitation effects of l-lactic acid on both periodontal ligament fibroblasts (PdLFs) and peripheral blood mononuclear cells (PBMCs). PdLFs and PBMCs were treated with 1-nonadecene and l-lactic acid. Cytokines’ expression was measured using quantitative real-time polymerase chain reaction (qRT-PCR). E-cadherin, N-cadherin, and macrophage polarization markers were measured using flow cytometry. The collagen, matrix metalloproteinase (MMP)-1, and released cytokines were measured using collagen assay, western blot, and Luminex assay, respectively. In PdLFs, 1-nonadecene enhances inflammation through the upregulation of some inflammatory cytokines including IL-1β, IL-6, IL-12A, monocyte chemoattractant protein (MCP)-1, and platelet-derived growth factor (PDGF) α. 1-Nonadecene also induced MET through the upregulation of E-cadherin and the downregulation of N-cadherin in PdLFs. 1-Nonadecene polarized macrophages to a pro-inflammatory phenotype and suppressed their cytokines’ release. l-lactic acid exerted a differential impact on the inflammation and proliferation markers. Intriguingly, l-lactic acid induced fibrosis-like effects by enhancing collagen synthesis, while inhibiting MMP-1 release in PdLFs. These results provide a deeper understanding of 1-nonadecene and l-lactic acid’s roles in modulating the microenvironment of the periapical area. Consequently, further clinical investigation can be employed for target therapy.

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“…Analysis and characterization of the markers were performed using BD FlowJo software (RRID:SCR_014422, V 10.8.0). The gating strategies was performed as previously described 68 .…”

Section: Methodsmentioning

confidence: 99%