The enzymic degradation of 3′, 5′ cyclic AMP in strains of E., coli sensitive and resistant to catabolite repression

Monard, Denis; Janeček, J.; Rickenberg, H.V.

doi:10.1016/0006-291x(69)90388-x

Cited by 72 publications

(32 citation statements)

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“…A great variety of effects of added cAMP, especially on catabolite repressed functions (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), have been seen in wildtype or adenylate cyclase-deficient mutants of several Gramnegative strains. No effects of added cAMP have been seen in Gram-positive species, although cAMP or adenylate cyclase have been detected in several Gram-positive species (4,7,(21)(22)(23).…”

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confidence: 99%

Adenosine 3′:5′-Cyclic Monophosphate as a Regulator of Bacterial Transformation

Wise

Alexander

Powers

1973

Proc. Natl. Acad. Sci. U.S.A.

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Adenosine 3': 5'-cyclic monophosphate added to exponentially growing cells of Hemophilus influenzae strain Rd increases competence for transformation 100-to 10,000-fold. Cyclic AMP added to near-stationary or stationary cells does not increase competence over the high level normally found in the early stationary phase.In most bacteria that can take up naked DNA and can incorporate genes of this DNA into its own chromosome, i.e., be transformed, highest levels of competence for transformation usually occur in late exponential phase and may persist into the stationary phase (1-3). Late exponential or stationary phase is also the time when highest concentrations of internal or external adenosine 3':5'-cyclic monophosphate (cAMP) occur in bacterial strains where this has been measured (4-7).A great variety of effects of added cAMP, especially on catabolite repressed functions (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), have been seen in wildtype or adenylate cyclase-deficient mutants of several Gramnegative strains. No effects of added cAMP have been seen in Gram-positive species, although cAMP or adenylate cyclase have been detected in several Gram-positive species (4,7,(21)(22)(23). Considering these facts it seemed reasonable to add cAMP to an exponentially growing culture of a transformable Gramnegative strain such as Hemophilus influenzae Rd (24,25) and to look for increased transformability. Our first experiment, using crude cell lysate DNA, succeeded in showing a 100-fold increase in competence with 1 mM cAMP. Subsequent improved techniques yield transformation frequencies up to 10,000-fold above the control level. The improvements especially include the use of saturating amounts of partially purified DNA. MATERIALS AND METHODSBacterial Strains. H. influenzae strain Rd of Alexander and Leidy (24) was the kind gift of R. M. Herriott. Spontaneous mutants of the strain resistant to 1000 Mug/ml of streptomycin or to 5 ,ug/ml of novobiocin (Cathomycin) were selected on supplemented brain-heart infusion (BHI) agar with the antibiotics. Strains were stored on supplemented BHI agar slants or plates at room temperature and were transferred every 5-7 days.Media. Cells were grown on solidified supplemented BHI agar or were added to molten (450) supplemented BHI agar.This agar is essentially as described by Goodgal and Herriott (25). Difco brain heart infusion broth was autoclaved and sterile hemin (25 Mg/ml) and sterile NAD (2 Mg/ml) were added to the cooled broth. 1% agar was added as required. Chemicals. cAMP was purchased from Sigma Co., Calbiochem, or P-L Biochemicals; cyclic GMP (cGMP), hemin, adenine nucleotides, and NAD were from Sigma Co. Beef heart 3':5' cyclic nucleotide phosphodiesterase (29) was a Sigma Co. product. Novobiocin (Upjohn) and streptomycin (Schwarz-Mann) were used. RESULTSEffects of cAMP on Transformation of Exponentially Growing Cells. As shown in Fig. 1, additions of cAMP as low as 0.1 mM resulted in a marked increase in transformation frequencies of exponentially growing H. i...

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confidence: 99%

Adenosine 3′:5′-Cyclic Monophosphate as a Regulator of Bacterial Transformation

Wise

Alexander

Powers

1973

Proc. Natl. Acad. Sci. U.S.A.

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“…This would contrast with a role in modulating the cellular level of CAMP, as described for other bacteria (Monard et al, 1969;Buettner et al, 1973;Alper & Ames, 1975;Botsford, 1981Botsford, , 1984. Although the bacterial cytoplasmic membrane is relatively impermeable to nucleotides, extracellular cAMP present at mM levels can leak into the cell in amounts sufficient to complement mutations in adenylate cyclase (e. g. Pastan & Adhya, 1976;Botsford, 1981); it can alter normal gene expression and can exert growthinhibitory and lethal effects in various Gram-negative bacteria (DeRobertis et al, 1973;Judewicz et al, 1974;Ackerman et al, 1974;Alper & Ames, 1975;Iuchi et al, 1975).…”

Section: Discussionmentioning

confidence: 97%

“…In bacteria, cellular cAMP USA. levels are apparently regulated by the activities of these two cytoplasmic enzymes, and by excretion of excess cAMP into the growth medium. However, the role of cAMP phosphodiesterase in this regulation has not been completely resolved (Monard et al, 1969 ;Buettner et al, 1973;Alper & Ames, 1975;Saier et al, 1975;Botsford, 1981Botsford, , 1984.…”

Section: Introductionmentioning

confidence: 99%

Growth of the marine luminous bacterium Vibrio fischeri on 3':5'-cyclic AMP: correlation with a periplasmic 3':5'-cyclic AMP phosphodiesterase

Dunlap¹,

Muêller²,

Lisa³

et al. 1992

Journal of General Microbiology

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The marine luminous bacterium Vibrio jischeri, the species-specific light-organ symbiont of monocentrid (pinecone) fish, grew on 3':5'-cyclic adenosine monophosphate (CAMP) as a sole source of carbon and energy, a capability not previously described in bacteria. In a minimal, chemically defined medium containing cAMP as the sole source of carbon and energy, V.jischeri cells grew more slowly than on glucose or ribose, but as quickly as on 5'-AMP. Expression of luminescence, which is dependent on cAMP in V.jischeri, was stimulated in cells grown on cAMP compared to cells grown on glucose or ribose. All strains of Kjischeri tested (MJ-I, B-61, ATCC 7744, MJ-A1, CG-A1) grew on CAMP, as did strains of two other marine luminous bacteria, V. fogei and Photobacterium phosphoreum, and strains of the terrestrial enteric bacterium Serratia marcescescens. Other tested species of marine luminous bacteria (P. feiognathi, Shewaneffa [ Afteromonas] hamhi, V. hmeyi, V. orientalis, V. splendidus) and terrestrial enteric bacteria (Escherichia cofi, Salmonella typhimurium), which grew on 5'-AMP or ribose, did not grow on CAMP. Assays on intact cells and periplasmic extracts revealed that V.jischeri MJ-1 produced a periplasmic 3': 5'-CAMP phosphodiesterase (CAMP phosphodiesterase) of very high specific activity 19.0 p o l phosphate released (mg protein)-' min-l ] and narrow substrate specificity (3': 5'-CAMP and 3': 5'-cGMP were attacked). The novel periplasmic location and unusually high activity of cAMP phosphodiesterase appear to account for the ability of this species to grow on CAMP. The periplasmic cAMP phosphodiesterase of Kjischeri might play a role in degrading free cAMP in seawater or in a CAMP-mediated aspect of the light organ symbiosis with monocentrid fish.

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“…The presence of cyclic 3', 5' nucleotide phosphodiesterase in bacteria has been reported for E. coli (15), B. liquefaciens (10) and Serratia marcescens (19). The phosphodiesterase of S. marcescens is known to be inhibited competitively by theophylline, Zn2+, Cu2+, or Ni2+, to be stimulated by Fe2+, Ca2+, or Ba2+, but not to require Mg2+ or Mn2+ for its activity.…”

Section: Hewlett Et Almentioning

confidence: 97%

Adenylate Cyclase Activity of Bordetella Organisms

Endoh

Takezawa

Nakase

1980

Microbiology and Immunology

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Abundant adenylate cyclase activity was found in the phase I cultures not only of Bordetella pertussis but also of B. parapertussis and B. bronchiseptica. The enzyme activity in the culture fluid increased rapidly and reached a peak during the logarithmic growth phase. B. parapertussis and B. bronchiseptica especially produced a high activity of the enzyme in the culture fluid during the logarithmic phase, but little or no activity was detected in the cells throughout the growth period. In the culture of B. pertussis, the intracellular activity was higher than that in the culture fluid. Phase III cultures of these species lacked both the extracellular and intracellular enzyme activities throughout their growth.In the culture of B. parapertussis, accumulation of cyclic AMP was parallel to that of adenylate cyclase activity through the growth periods, but in B. pertussis there was no parallelism from the stationary through the declining phases. The difference in production patterns of the enzyme activity among the species is discussed.

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The enzymic degradation of 3′, 5′ cyclic AMP in strains of E., coli sensitive and resistant to catabolite repression

Cited by 72 publications

References 9 publications

Adenosine 3′:5′-Cyclic Monophosphate as a Regulator of Bacterial Transformation

Adenosine 3′:5′-Cyclic Monophosphate as a Regulator of Bacterial Transformation

Growth of the marine luminous bacterium Vibrio fischeri on 3':5'-cyclic AMP: correlation with a periplasmic 3':5'-cyclic AMP phosphodiesterase

Adenylate Cyclase Activity of Bordetella Organisms

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