The entire nucleotide sequence of foxtail mosaic virus RNA

Bancroft, J.B.; Rouleau, Michèle; Johnston, R.; Prins, Leonard J.; Mackie, George A.

doi:10.1099/0022-1317-72-9-2173

Cited by 80 publications

(46 citation statements)

References 44 publications

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“…It can infect at least 56 plant species in the Gramineae as well as a number of species in 11 dicotyledonous families [27,28]. The genome of FoMV consists of a capped, messenger sense ssRNA of 6,151 nucleotides in length and poly(A) tail [5]. The genomic arrangement of FoMV is similar to potato virus X (PVX) and other potexviruses with five major open reading frames (ORF) with presumed corresponding functions (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…1). This ORF continues in-frame into the CP gene so that an amino-terminal extended form of CP might be produced during infection [5]. In fact, a larger protein with serologically related to the CP has been detected by in vitro translation of FoMV genomic RNA [24].…”

Section: Introductionmentioning

confidence: 99%

“…The nucleotide positions are based on the published complete genomic sequence of FoMV [5]. Primers used to amplify the 5 -and 3 -FoMV genomic segments separately included two sets of primers.…”

Section: Construction Of Fomv Full-length Clonesmentioning

confidence: 99%

“…The coat protein gene (ORF 5; CP) is located at the 3 -terminus encoding a 24 kDa protein, although on SDS gels it appears as a ca 30 kDa protein [24]. It is expressed from a 0.9 kb subgenomic (sg) RNA species [5,24].…”

Section: Introductionmentioning

confidence: 99%

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The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

Robertson

French

Morris

2000

Archives of Virology

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Summary. Infectious transcripts were successfully derived from full-length cDNA clones of foxtail mosaic potexvirus (FoMV). Full-length clones were constructed by RT-PCR whereby 5 and 3 genomic segments of 2.7 and 3.4 kb, respectively, were ligated into Bluescript II KS. The in vitro RNA transcripts were infectious to moncotyledonous (barley) and dicotyledonous (Chenopodium amaranticolor) plant species. Individual mutation studies on clones of each of the five major ORFs confirmed predicted gene function for the polymerase, TGB (triple gene block), and coat protein (CP) genes. Protoplast studies on expression of a unique open reading frame, ORF 5A, which initiates 143 nts upstream of the CP before it "reads through" the CP, revealed that the 5A protein was produced in vivo. Mutation analysis of the 5A ORF indicated, however, that it was not required for either replication or for productive infection of plants. However, the nucleic acid sequences encoding the extended CP segment were shown to be important for CP expression. Additional mutations in 5A had no effect on FoMV replication in protoplasts but rendered the virus noninfectious to plants. A correlation with diminished CP production from both mutant clones implies that synthesis of subgenomic CP mRNA was compromised, and this limited systemic infection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Construction Of Fomv Full-length Clonesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

Robertson

French

Morris

2000

Archives of Virology

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“…[26 -28], foxtail mosaic virus (FoMV) [29,30], four strains of wheat streak mosaic virus (WSMV) [31], four strains of Johnsongrass mosaic virus (JGMV) from Australia, Kansas, Nigeria, and Israel [32][33][34], and high plains virus (HPV) from Kansas and Idaho [35]. We have previously described isolation and purification of the coat proteins from these viruses by ultracentrifugal precipitation through multiple sucrose density gradients.…”

mentioning

confidence: 99%

Formation of (b_n−1 + H₂O) ions by collisional activation of maldi-formed peptide [M + H]⁺ ions in a QqTOF mass spectrometer

She

Krokhin

Spicer

et al. 2007

J. Am. Soc. Mass Spectrom.

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Collisional activation of [M ϩ H] ϩ parent ions from peptides of n amino acid residues may yield a rearrangement that involves loss of the C-terminal amino acid residue to produce (b nϪ1 ϩ H 2 O) daughters. We have studied this reaction by a retrospective examination of the m/z spectra of two collections of data. The first set comprised 398 peptides from coat protein digests of a number of plant viruses by various enzymes, where conditions in the tryptic digests were chosen so as to produce many missed cleavages. In this case, a large effect was observed-323 (b nϪ1 ϩ H 2 O) daughter ions (ϳ81%), including 185 (ϳ46%) "strong" decays with ratios (b nϪ1 ϩ H 2 O)/ (b nϪ1 ) Ͼ 1. The second set comprised 1200 peptides, all from tryptic digests, which were carried out under more stringent conditions, resulting in relatively few missed cleavages. Even here, 190 (b nϪ1 ϩ H 2 O) ions (ϳ16%) were observed, including 87 (Ͼ 7%) "strong" decays, so the effect is still appreciable. The results suggest that the tendency for (b nϪ1 ϩ H 2 O) ion formation is promoted by the protonated side chain of a non-C-terminal basic amino acid residue, in the order arginine ӷ lysine Ն histidine, and that its (non-C-terminal) position is not critical. The results can be interpreted by a mechanism in which hydrogen bonding between the protonated side chain and the (n Ϫ 1) carbonyl oxygen facilitates loss of the C-terminal amino acid residue to give a product ion having a carboxyl group at the new

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Discovery of a novel potexvirus in the seagrass Thalassia testudinum from Tampa Bay, Florida

Bogaert

Rosario

Furman

et al. 2018

Limnol Oceanogr Letters

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Seagrass meadows are important coastal ecosystems that are declining worldwide. Given the profound impact of the microbiome on plant health, exploration of the seagrass microbiome is critical for proper ecosystem management and conservation. Although prior studies have investigated seagrass‐associated bacteria, fungi, and protists, virtually nothing is known about viruses infecting these flowering marine plants. Here, we report genomic and microscopic evidence of a new positive‐sense, single‐stranded ribonucleic acid virus infecting apparently healthy Thalassia testudinum in Florida. The virus, named turtlegrass virus X (TVX), shares 66% genome‐wide pairwise identity with foxtail mosaic virus, a potexvirus that infects terrestrial grasses. Quantitative polymerase chain reaction revealed TVX presence in 10% to 80% of T. testudinum leaves from two Tampa Bay sampling locations in February and August 2017, with an average viral load of 4.65 × 108 copies per mg leaf tissue. The discovery of TVX advances seagrass microbiome research, prompting further studies to assess its ecological impact.

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The entire nucleotide sequence of foxtail mosaic virus RNA

Cited by 80 publications

References 44 publications

The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

Formation of (b_n−1 + H₂O) ions by collisional activation of maldi-formed peptide [M + H]⁺ ions in a QqTOF mass spectrometer

Discovery of a novel potexvirus in the seagrass Thalassia testudinum from Tampa Bay, Florida

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The entire nucleotide sequence of foxtail mosaic virus RNA

Cited by 80 publications

References 44 publications

The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

Formation of (bn−1 + H2O) ions by collisional activation of maldi-formed peptide [M + H]+ ions in a QqTOF mass spectrometer

Discovery of a novel potexvirus in the seagrass Thalassia testudinum from Tampa Bay, Florida

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Formation of (b_n−1 + H₂O) ions by collisional activation of maldi-formed peptide [M + H]⁺ ions in a QqTOF mass spectrometer