The entire nucleotide sequence of baboon endogenous virus DNA : A chimeric genome structure of murine type C and simian type D retroviruses.

Kato, Shingo; Matsuo, Koichi; Nishimura, Naoyuki; Takahashi, Naomi; Takano, Toshiya

doi:10.1266/jjg.62.127

Cited by 59 publications

(52 citation statements)

References 28 publications

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“…Some highly conserved amino acid motifs for mammalian type C retroviruses are present in the Gag-Pol polyproteins of PERV-B(33) and PERV-B (43). Identical to the situation in PERV-MSL (1) and similar to other type C retroviruses, including GaLV (18) and BaEV (35), the consensus sequence for the N terminus of p30 starts at nt 1736 with a proline (48), and the C-terminal end is located at nt 2501 to 2512 (Thr-Lys-Ile-Leu). Amino acid comparisons with the p30 and p27 capsid proteins of other retroviruses revealed scores of 96.1% (PERV-MSL), 75.7% (GaLV), 68.2% (BaEV), 61.7% (FeLV), and 61.1% (MoMLV).…”

Section: Resultsmentioning

confidence: 93%

“…The sequences used for homology studies are PERV-MSL (AF038600) (1), Tsukuba-1 (AF038599) (1), PK-15 ERV (AF038601) (1), Moloney murine leukemia virus (MoMLV) (J02255, J02256, and J02257) (59), gibbon ape leukemia virus (GaLV) (M26927) (18), baboon endogenous virus (BaEV) (D10032, D00088, and N00088) (35), FeLV (M18247 and M19392) (21), and human endogenous retrovirus ERV3 pol-env 3Ј LTR (M12140) (14).…”

Section: Methodsmentioning

confidence: 99%

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Establishment and Characterization of Molecular Clones of Porcine Endogenous Retroviruses Replicating on Human Cells

Czauderna

Fischer

Boller

et al. 2000

J Virol

105

109

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The use of pig xenografts is being considered to alleviate the shortage of allogeneic organs for transplantation. In addition to the problems overcoming immunological and physiological barriers, the existence of numerous porcine microorganisms poses the risk of initiating a xenozoonosis. Recently, different classes of type C porcine endogenous retoviruses (PERV) which are infectious for human cells in vitro have been partially described. We therefore examined whether completely intact proviruses exist that produce infectious and replication-competent virions. Several proviral PERV sequences were cloned and characterized. One molecular PERV class B clone, PERV-B(43), generated infectious particles after transfection into human 293 cells. A second clone, PERV-B(33), which was highly homologous to PERV-B(43), showed a G-to-A mutation in the first start codon (Met to Ile) of the env gene, preventing this provirus from replicating. However, a genetic recombinant, PERV-B(33)/ATG, carrying a restored env start codon, became infectious and could be serially passaged on 293 cells similar to virus clone PERV-B(43). PERV protein expression was detected 24 to 48 h posttransfection (p.t.) using cross-reacting antiserum, and reverse transcriptase activity was found at 12 to 14 days p.t. The transcriptional start and stop sites as well as the splice donor and splice acceptor sites of PERV mRNA were mapped, yielding a subgenomic env transcript of 3.1 kb. PERV-B(33) and PERV-B(43) differ in the number of copies of a 39-bp segment in the U3 region of the long terminal repeat. Strategies to identify and to specifically suppress or eliminate those proviruses from the pig genome might help in the production of PERV-free animals.

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Section: Resultsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Establishment and Characterization of Molecular Clones of Porcine Endogenous Retroviruses Replicating on Human Cells

Czauderna

Fischer

Boller

et al. 2000

J Virol

105

109

View full text Add to dashboard Cite

show abstract

“…5B). RD-114 is genetically related to BaEV, and both of these viruses are recombinant viruses that contain a type C (gammaretrovirus group) ORF for gag-pol and a type D (betaretrovirus group) env gene, and supposedly they arose from cross-species transmission (1,14,36). The amino acid identity was 98.6% for Pol proteins of ERV-DC18 and RD-114 SC3C and 81.1% for the TM regions of Env proteins of ERV-DC18 and FeLV-A clone 61E (55.8% for the entire protein).…”

Section: Characterization Of Erv-dcsmentioning

confidence: 99%

Infectious Endogenous Retroviruses in Cats and Emergence of Recombinant Viruses

Anai

Ochi

Watanabe

et al. 2012

J Virol

189

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bEndogenous retroviruses (ERVs) comprise a significant percentage of the mammalian genome, and it is poorly understood whether they will remain as inactive genomes or emerge as infectious retroviruses. Although several types of ERVs are present in domestic cats, infectious ERVs have not been demonstrated. Here, we report a previously uncharacterized class of endogenous gammaretroviruses, termed ERV-DCs, that is present and hereditary in the domestic cat genome. We have characterized a subset of ERV-DC proviral clones, which are numbered according to their genomic insertions. One of these, ERV-DC10, located in the q12-q21 region on chromosome C1, is an infectious gammaretrovirus capable of infecting a broad range of cells, including human. Our studies indicate that ERV-DC10 entered the genome of domestic cats in the recent past and appeared to translocate to or reintegrate at a distinct locus as infectious ERV-DC18. Insertional polymorphism analysis revealed that 92 of 244 domestic cats had ERV-DC10 on a homozygous or heterozygous locus. ERV-DC-like sequences were found in primate and rodent genomes, suggesting that these ERVs, and recombinant viruses such as RD-114 and BaEV, originated from an ancestor of ERV-DC. We also found that a novel recombinant virus, feline leukemia virus subgroup D (FeLV-D), was generated by ERV-DC env transduction into feline leukemia virus in domestic cats. Our results indicate that ERV-DCs behave as donors and/or acceptors in the generation of infectious, recombinant viruses. The presence of such infectious endogenous retroviruses, which could be harmful or beneficial to the host, may affect veterinary medicine and public health.

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“…Intact genomes of baboon endogenous virus (BaEV) are present in the papionini monkeys (except macaques) and in four species of African green monkey (Cercopithecus aethiops) (7,8). Mammals appear to have retained the presence of at least some copies of non-defective genomic retroviruses, such as intracytoplasmic A-type particles (IAPs) and type C endogenous retroviruses (6,(9)(10)(11)(12). It is difficult to explain the selective pressure that maintains these ERVs in the genome.…”

Section: Features Of Endogenous Retrovirusesmentioning

confidence: 99%

Evidence for expression of endogenous retroviral sequences on primate reproductive tissues and detection of cross-reactive ERVS antigens in the baboon ovary: a review

Arimi

Nyachieo²,

Langat³

et al. 2006

E Af Med Jrnl

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The entire nucleotide sequence of baboon endogenous virus DNA : A chimeric genome structure of murine type C and simian type D retroviruses.

Cited by 59 publications

References 28 publications

Establishment and Characterization of Molecular Clones of Porcine Endogenous Retroviruses Replicating on Human Cells

Establishment and Characterization of Molecular Clones of Porcine Endogenous Retroviruses Replicating on Human Cells

Infectious Endogenous Retroviruses in Cats and Emergence of Recombinant Viruses

Evidence for expression of endogenous retroviral sequences on primate reproductive tissues and detection of cross-reactive ERVS antigens in the baboon ovary: a review

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