The Emerging Role of Extracellular Vesicles Detected in Different Biological Fluids in COPD

Neri, Tommaso; Celi, Alessandro; Tinè, Mariaenrica; Bernardinello, Nicol; Cosio, Manuel G.; Saetta, Marina; Nieri, Dario; Bazzan, Erica

doi:10.3390/ijms23095136

Cited by 5 publications

(3 citation statements)

References 74 publications

(90 reference statements)

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“…Extracellular vesicles (EVs) are lipid bilayer structures secreted by living cells and are classified into either exosomes, microvesicles (MVs), or apoptotic bodies, based on the intracellular production mechanism and size ( Figure 1 ) [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , …”

Section: Introductionmentioning

confidence: 99%

Regulation of Extracellular Vesicle-Mediated Immune Responses against Antigen-Specific Presentation

Matsuzaka

Yashiro

2022

Vaccines

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Extracellular vesicles (EVs) produced by various immune cells, including B and T cells, macrophages, dendritic cells (DCs), natural killer (NK) cells, and mast cells, mediate intercellular communication and have attracted much attention owing to the novel delivery system of molecules in vivo. DCs are among the most active exosome-secreting cells of the immune system. EVs produced by cancer cells contain cancer antigens; therefore, the development of vaccine therapy that does not require the identification of cancer antigens using cancer-cell-derived EVs may have significant clinical implications. In this review, we summarise the molecular mechanisms underlying EV-based immune responses and their therapeutic effects on tumour vaccination.

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Section: Introductionmentioning

confidence: 99%

Regulation of Extracellular Vesicle-Mediated Immune Responses against Antigen-Specific Presentation

Matsuzaka

Yashiro

2022

Vaccines

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“…Up till now, circulating EVs as promising biomarkers and potential therapeutics in COPD patients have been widely discussed [ 11 , 12 ]. EVs, including those derived from inhaled bacteria, macrophages, epithelial cells, and endothelial cells, are related to COPD development and exacerbation to some extent [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Extracellular vesicles isolated from hyperuricemia patients might aggravate airway inflammation of COPD via senescence-associated pathway

Liu

Li²,

Zheng³

et al. 2022

J Inflamm

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Backgrounds Chronic obstructive pulmonary disease (COPD) is a major health issue resulting in significant mortality worldwide. Due to the high heterogeneity and unclear pathogenesis, the management and therapy of COPD are still challenging until now. Elevated serum uric acid(SUA) levels seem to be associated with the inflammatory level in patients with COPD. However, the underlying mechanism is not yet clearly established. In the current research, we aim to elucidate the effect of high SUA levels on airway inflammation among COPD patients. Methods Through bioinformatic analysis, the common potential key genes were determined in both COPD and hyperuricemia (HUA) patients. A total of 68 COPD patients aged 50—75-year were included in the study, and their clinical parameters, including baseline characteristics, lung function test, as well as blood chemistry test were recorded. These parameters were then compared between the COPD patients with and without HUA. Hematoxylin & Eosin (HE), immunofluorescence (IF), and Masson trichrome staining were performed to demonstrate the pathological changes in the lung tissues. Furthermore, we isolated extracellular vesicles (EVs) from plasma, sputum, and bronchoalveolar lavage fluid (BALF) samples and detected the expression of inflammatory factor (Interleukin-6 (IL-6), IL-8 and COPD related proteases (antitrypsin and elastase) between two groups. Additionally, we treated the human bronchial epithelial (HBE) cells with cigarette smoke extract (CSE), and EVs were derived from the plasma in vitro experiments. The critical pathway involving the relationship between COPD and HUA was eventually validated based on the results of RNA sequencing (RNA-seq) and western blot (WB). Results In the study, the COPD patients co-existing with HUA were found to have more loss of pulmonary function compared with those COPD patients without HUA. The lung tissue samples of patients who had co-existing COPD and HUA indicated greater inflammatory cell infiltration, more severe airway destruction and even fibrosis. Furthermore, the high SUA level could exacerbate the progress of airway inflammation in COPD through the transfer of EVs. In vitro experiments, we determined that EVs isolated from plasma, sputum, and BALF played pivotal roles in the CSE-induced inflammation of HBE. The EVs in HUA patients might exacerbate both systemic inflammation and airway inflammatory response via the senescence-related pathway. Conclusion The pulmonary function and clinical indicators of COPD patients with HUA were worse than those without HUA, which may be caused by the increased airway inflammatory response through the EVs in the patient's peripheral blood. Moreover, it might mediate the EVs via senescence-related pathways in COPD patients with HUA.

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“…The text by Tomaso Neri et al [ 9 ] emphasizes the emerging role of extracellular vesicles in COPD and their detection in various biological fluids. The most important message of this text is that chronic obstructive pulmonary disease (COPD) involves complex cellular and molecular mechanisms that are not yet fully understood.…”

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confidence: 99%

The Role of Extracellular Vesicles in Aging and Disease

Borrás,

Mas-Bargues

2023

IJMS

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The Emerging Role of Extracellular Vesicles Detected in Different Biological Fluids in COPD

Cited by 5 publications

References 74 publications

Regulation of Extracellular Vesicle-Mediated Immune Responses against Antigen-Specific Presentation

Regulation of Extracellular Vesicle-Mediated Immune Responses against Antigen-Specific Presentation

Extracellular vesicles isolated from hyperuricemia patients might aggravate airway inflammation of COPD via senescence-associated pathway

The Role of Extracellular Vesicles in Aging and Disease

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