2010
DOI: 10.1186/1742-4690-7-s1-p176
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Abstract: On the ground of the most recent evidences regarding the role of some malaria Plasmodia which have primates as natural reservoirs, the Authors focused their attention on the emerging species Plasmodium knowlesi.

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“…The melting points were measured in capillary tubes on a SGW X-4 melting point apparatus without correction. 1 H nuclear magnetic resonance (NMR) spectroscopy was performed on a Bruker AMX-400 MHz NMR and Ascend 600 MHz NMR (TMS as the internal standard). Chemical shifts were reported in parts per million (ppm, δ) downfield from TMS.…”
Section: ■ Experimental Sectionmentioning
confidence: 99%
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“…The melting points were measured in capillary tubes on a SGW X-4 melting point apparatus without correction. 1 H nuclear magnetic resonance (NMR) spectroscopy was performed on a Bruker AMX-400 MHz NMR and Ascend 600 MHz NMR (TMS as the internal standard). Chemical shifts were reported in parts per million (ppm, δ) downfield from TMS.…”
Section: ■ Experimental Sectionmentioning
confidence: 99%
“…Then, the organic phase was evaporated by vacuum evaporation, and the remaining solids were separated and purified by silica gel (Greagent, 01541589, 200−300 mesh) column chromatography [eluted with 0−25% ethyl acetate/petroleum ether (EA/PE, boiling range of PE: 60−90 °C)] to obtain a yellow solid tert-butyl 6-(5-(ethoxycarbonyl)pyrimidin-2-yl)-2,6-diazaspiro [3.4]octane-2-carboxylate (33.3 g, 92 mmol). 1 H NMR (400 MHz, CDCl 3 -d 6 ): δ 8.86 (s, 2H), 4.34 (q, J = 7.1 Hz, 2H), 3.94 (d, J = 8.6 Hz, 2H), 3.88 (d, J = 8.6 Hz, 2H), 3.80 (s, 2H), 3.70 (t, J = 6.9 Hz, 2H), 2.22 (t, J = 6.9 Hz, 2H), 1.45 (s, 9H), 1.36 (t, J = 7.1 Hz, 3H).…”
Section: ■ Experimental Sectionmentioning
confidence: 99%
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