The Electrochemical Detection of Dopamine Based on the Cobalt-Modified Nitrogen Doping Carbon Aerogels from Biomass

Abstract

The sensitive detection of dopamine (DA), especially the accurate monitoring of cell secreted DA, is necessary to the study of pathogenesis of central nervous system disease. Here, the cobalt-modified nitrogen doping carbon aerogels (Co-NCA) with three-dimensional pores were successfully synthesized through the hydrothermal and freeze-drying method of the biomass. As a natural substance, starch is the carbon source of Co-NCA, which is environmentally friendly. The electrochemically active area of an electrode modified by Co-NCA was about 1.53 times that of carbon aerogels. Benefiting from the Co and N element, the Co-NCA modified electrode (Co-NCA/GCE) showed that Co-NCA had a wider linear range (0.2-200 and 200-1000 μM) and a lower detection limit (55.6 nM) towards DA. The reproducibility and repeatability of Co-NCA/GCE for detecting 500 M DA was 2.21% and 0.797%, respectively. After being stored at 4°C for 7 days, the current response of Co-NCA/GCE still maintained 96.5% of the initial current value. Finally, the Co-NCA/GCE was successfully applied to the monitoring of DA, which was released from PC12 cells after stimulated by 50 mM K+.

Convenient and Accurate Detection of Dopamine and Glucose by Modifying Carbon Fiber Electrodes

“…30,31 It has become an important way to improve the performance of the detection sensor. [32][33][34][35] For example, Zhao et al found that carbon nanotube modified carbon-fiber electrodes had a better distinction for uric acid, DA and ascorbic acid, compared with pure carbon fiber electrodes; 36 Zhao et al used Ni(OH) 2 modified carbon fiber sensors combined with artificial intelligence to achieve the goal of efficient glucose detection. 37 At the same time, many studies have shown that ZnO is a non-toxic and biocompatible semiconductor material that is often used as a load for sensors due to its high potential and promising adsorption of biological macromolecules.…”

Dual-Signal Colorimetric and Electrochemical Sensor of Dopamine Based on Nanocomposite of Cobalt Oxyhydroxide/Carbon Black

“…[4][5][6] Consequently, it is significant to develop a convenient, sensitive and reliable method to detect dopamine in complex biological samples, especially the secreted from living cell. [7][8][9][10] In recent years, the dual-mode detection strategy attracted extensive attention and has been proved to be more effective than the single-mode method. [11][12][13][14][15] A variety of analytical methods, such as surface-enhanced Raman spectroscopy, fluorescence and high performance liquid chromatography have been applied to the analysis of DA.…”