The effects of transient systemic hypotension on renal oxidative status, morphology and plasma nitric oxide levels in pregnant rats

Bayrak, Sibel; Pehlivanoğlu, Bilge; Balkanci, Zeynep Dicle; Özyürek, Hamìt; Aksoy, Yasemin; Atilla, Pergin; Çakar, Ayşe Nur

doi:10.1080/14767050902822229

Cited by 5 publications

(4 citation statements)

References 45 publications

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“…Oxytocin infusions were used prophylactically at obstetric request in 45 women (75%), at a standardised dose of 10 IU in 125 mL every hour for the first 4 h. Urine output during the first 6 h and at 12 and 18 h, according to body weight, are shown in Table 1, for the whole group and according to whether oxytocin infusion was used. Median (95% CI) 24-h intravenous fluid input were 2.8 (1.8-4.3) L. Median (IQR [range]) times to first oral fluids and cessation of intravenous fluid were 3 (2-4 [2][3][4][5][6][7][8][9][10][11]) h and 6 (5-7 [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]) h. Mean (±SD) haemoglobin concentrations on the day of surgery and the following day were 11.9 (±1.2) and 10.5 (±1.0) g/dL, respectively. The mean (±SD) drop in haemoglobin concentration with or without oxytocin infusion was 1.4 (±0.9) and 1.4 (±0.4) mg/dL respectively; no patient received blood products.…”

Section: Resultsmentioning

confidence: 99%

“…6 Conditions such as preeclampsia may involve renal impairment, and various potential influences on renal function such as neuraxial anaesthesia, blood loss, and drugs such as non-steroidal anti-inflammatory drugs and oxytocin during or after labour and delivery may be significant. [7][8][9] Without accurate information on normal urine output, it is hard to set goals for women who may be at risk of renal dysfunction. The aim of this observational study was to establish a normal range for urine output after elective caesarean section under neuraxial anaesthesia in healthy women.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Normal urine output after elective caesarean section: an observational study

Mackenzie

Woolnough

Barrett

et al. 2010

International Journal of Obstetric Anesthesia

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Normal urine output after elective caesarean section: an observational study

Mackenzie

Woolnough

Barrett

et al. 2010

International Journal of Obstetric Anesthesia

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“…The catheterised femoral artery was joined to a pressure transducer (BIOPAC SS13L, BIOPAC Systems Inc., California, USA), which was connected to a data acquisition/analysis system (BIOPAC MP30, BIOPAC Systems Inc., California, USA) to monitor blood pressure. After a 30-minute stabilisation period following catheterisation, systemic hypotension was induced by blood withdrawal (approximately 1 mL per 100 g body weight) within 5 minutes in hypotension groups, as described previously (10,11). As clamping of the uterine vasculature for 30 minutes was previously reported to be sufficient to cause damage in the foetal brain (12), mean arterial blood pressure (MABP) was maintained at 50 mmHg for a period of 30 minutes.…”

Section: Experimental Protocolmentioning

confidence: 99%

Effect of Transient Maternal Hypotension on Apoptotic Cell Death in Foetal Rat Brain

Özyürek¹,

Bayrak²,

Pehlivanoğlu³

et al. 2014

Balkan Med J

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Maternal hypotension during gestation is associated with foetal brain malformations (1-3). The brain stem and midbrain are particularly vulnerable to severe maternal hypotension. Intrauterine hypoxic/ischemic injury as a result of reduced utero-placental blood flow has been linked to serious neurological deficits in the offspring including cranial nerve palsies, abnormal brain stem-evoked responses, and cerebral palsy (4). Furthermore, several case reports demonstrate facial limb hypogenesis syndrome (5), arthrogryposis multiplex (6), and paraplegia (7) associated with maternal hypotension. Therefore, cerebral hypoxic/ischemic injury in the foetus induced by maternal hypotension contributes to neonatal morbidity and mortality (8).Although these various outcomes have been known for years, the pathophysiology of foetal brain malformations related to maternal perfusion failure is still unclear. Necrosis and apoptosis have been proposed as possible pathways that lead to cerebral pathology in the foetus (3, 9). Not only the form and the extent of neuronal death but also the time course of the injury depend on the severity of the ischemic insult (9). Accordingly, we previously observed a significant increase in apoptosis in the hippocampal region, periventricular matrix, and cerebral cortex of newborns on postnatal days 1 and 28, which was dependent on the timing and severity of intrauterine hypotension (3, 10). As mentioned above, the effects of maternal hypotension on development of the foetal brain have been reported in a limited number of studies in the postnatal period, but, to our knowledge, not in the antenatal period. Therefore, we aimed to investigate the effects of transient systemic maternal hypotension on apoptotic cell death in the inBackground: Intrauterine perfusion insufficiency induced by transient maternal hypotension has been reported to be associated with foetal brain malformations. However, the effects of maternal hypotension on apoptotic processes in the foetal brain have not been investigated experimentally during the intrauterine period. Aims: The aim of this study was to investigate the effects of transient maternal hypotension on apoptotic cell death in the intrauterine foetal brain. Study Design: Animal experimentation. Methods: Three-month-old female Wistar albino rats were allocated into four groups (n=5 each). The impact of hypoxic/ischemic injury induced by transient maternal hypotension on the 15th day of pregnancy (late gestation) in rats was investigated at 48 (H17 group) or 96 hours (H19 group) after the insult. Control groups underwent the same procedure except for induction of hypotension (C17 and H17 groups). Brain sections of one randomly selected foetus from each pregnant rat were histopathologically evaluated for hypoxic/ischemic injury in the metencephalon, diencephalon, and telencephalon by terminal transferase-mediated dUTP nick end labelling and active cysteine-dependent aspartate-directed protease-3 (caspase-3) positivity for cell death. Results: The number of terminal tra...

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“…The body temperature was monitored and maintained at 37 °C during anesthesia. The hypotension procedure was performed on day 15 of gestation as described previously (22). Briefly, the right femoral artery was cannulated and attached to a pressure transducer (BIOPAC SS13L, BIOPAC Systems Inc., CA, USA).…”

Section: Animals and Experimental Protocolmentioning

confidence: 99%

Evaluation of apoptotic cell death following transient maternal hypotension in fetal rat brain: temporal pattern within the first 24 h after procedure

Bayrak

Pehlivanoğlu²,

Sevgili³

et al. 2014

Turk J Med Sci

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Background/aim: To explore the effects of maternal transient systemic hypotension on apoptotic neuronal death in an intrauterine fetal rat brain during the first 24 h after induction of hypotension. Materials and methods:A total of 40 pregnant Sprague-Dawley rats were subjected to either transient systemic hypotension produced for 30 min by blood withdrawal via femoral artery catheterization (hypotension group) or sham operation (control group) on day 15. Two randomly selected fetuses were taken from each rat at 0, 6, 12, and 24 h after the procedure. Apoptosis was evaluated in sections from the whole fetal brain by TUNEL and caspase-3 staining.Results: TUNEL (+) and caspase (+) cells were detected only on the walls of the ventricles of both groups and more abundantly in the hypotension groups than in the control groups at all time points (P < 0.05). The increase in TUNEL (+) and caspase (+) cells was highest at 12 h (P < 0.05) following hypotension compared to the other hypotension groups. Conclusion:Maternal transient systemic hypotension caused the hypoxia-ischemia (HI)-induced death of immature neurons by apoptosis, and this is especially prominent at 12 h after the insult. Determination of the susceptibility of a developing brain to HI at a certain time may have potential significance on the timing of neuroprotective measures.

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The effects of transient systemic hypotension on renal oxidative status, morphology and plasma nitric oxide levels in pregnant rats

Cited by 5 publications

References 45 publications

Normal urine output after elective caesarean section: an observational study

Normal urine output after elective caesarean section: an observational study

Effect of Transient Maternal Hypotension on Apoptotic Cell Death in Foetal Rat Brain

Evaluation of apoptotic cell death following transient maternal hypotension in fetal rat brain: temporal pattern within the first 24 h after procedure

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