2016
DOI: 10.1186/s12864-015-2267-9
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: BackgroundRNA interference (RNAi) is a powerful platform utilized to target transcription of specific genes and downregulate the protein product. To achieve effective silencing, RNAi is usually applied to cells or tissue with a transfection reagent to enhance entry into cells. A commonly used control is the same transfection reagent plus a “noncoding RNAi”. However, this does not control for the genomic response to the transfection reagent alone or in combination with the noncoding RNAi. These control effects … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

0
13
0

Year Published

2016
2016
2024
2024

Publication Types

Select...
8

Relationship

2
6

Authors

Journals

citations
Cited by 17 publications
(13 citation statements)
references
References 41 publications
0
13
0
Order By: Relevance
“…1). This could indicate a general (energy-consuming) RNA interference effect responding to the exogenous scrambled siRNA, but not to vehicle-only (36)(37)(38)(39), alongside the NNMT metabolic effects that are missed without vehicle.…”
Section: Discussionmentioning
confidence: 99%
“…1). This could indicate a general (energy-consuming) RNA interference effect responding to the exogenous scrambled siRNA, but not to vehicle-only (36)(37)(38)(39), alongside the NNMT metabolic effects that are missed without vehicle.…”
Section: Discussionmentioning
confidence: 99%
“…Our research group recently identified 213 differentially expressed genes that are modulated as a result of transfection with PEI only. Major genes that are linked to vascular dysregulation primarily include inflammation‐related and cell proliferative alterations (e.g., IL1A, STAT‐4, CCL8, SFN, and CSF2) (37). These data are supported by our current findings of increased CD68 + macrophage infiltration at 24 h of all PEI combined treatment groups compared with saline.…”
Section: Discussionmentioning
confidence: 99%
“…These genes were mostly related to inflammation and immune response. These findings illustrate how PEI alone could affect the results of the RNAi therapy [ 29 ].…”
Section: Anatomical Proximity Of Ec and Vsmcmentioning
confidence: 93%
“…In order to achieve a transfection success across all VSMC layers, a high dose of RNAi oligomers as well as potent transfection agents [e.g. liposomal or cationic polymer based such as polyethyleneimine (PEI)] may have to be used [ 9 , 28 , 29 ]. This again may result in inadvertent transfection of other cells residing in the vascular wall including EC, pericytes, dendritic cells and fibroblasts.…”
Section: Anatomical Proximity Of Ec and Vsmcmentioning
confidence: 99%
See 1 more Smart Citation