The effects of methyltestosteone on reproductive function in male greyhounds

Freshman, Joni L.; Olson, P N; Amann, R. P.; Carlson, E.D.; Twedt, D C; Bowen, Richard A.

doi:10.1016/0093-691x(90)90067-4

Cited by 10 publications

(8 citation statements)

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“…1). As their poor semen quality failed to improve in response to injection with GnRH-analogue or hCG, they were adopted as the subjects of this study.High doses or long-term treatment with PMSG inhibits pituitary function and spermatogenesis in the dog [1,2], and continuous treatment with PMSG is known to induce the production of anti-gonadotropin antibody in the blood of animals, except mares [12]. Therefore, in the present study, the dogs were given only three intramuscular injections of 50 mg T enanthate-depot (Testoviron-Depot, Schering AG Co., Ltd., Germany) plus 250 IU PMSG (Serotropin, Teikoku-zoki, Co., Ltd., Japan) at 2-week…”

mentioning

confidence: 99%

Changes in Plasma Testosterone and Testicular Transferrin Concentration, Testicular Histology and Semen Quality after Treatment of Testosterone-Depot plus PMSG to 3 Dogs with Asthenozoospermia.

Kawakami

Hori

Tsutsui

2000

The Journal of Veterinary Medical Science

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Three dogs diagnosed as having asthenozoospermia were given three intramuscular injections of 50 mg testosterone(T)-depot plus 250 IU pregnant mare serum gonadotropin (PMSG) at 2-week intervals, and their plasma T and testicular transferrin (Tf) concentrations, testicular histology, and semen quality were examined during the period of hormone therapy. Plasma T concentrations temporarily increased, and there was a slight improvement in spermatogenesis. Increased Tf concentrations suggested that Sertoli cell function in all three dogs was promoted by hormone treatment. The results showed that semen quality, especially the percentages of motile sperm and abnormal sperm, were improved between 1 and 5 weeks after the start of T-depot plus PMSG treatment.-KEY WORDS: asthenozoospermia, canine, testosterone plus PMSG treatment. J. Vet. Med. Sci. 62(2): 203-206, 2000 intervals. Peripheral blood samples were collected 4 times a day at-7,-1, 1, 3, 7, and 14 days after the start of hormone treatment. Plasma T concentrations were measured by radioimmunoassay, as reported previously [7]. The right testis and the left testis were biopsed under halothane inhalation anesthesia 1 week before and 5 weeks after the start of hormone treatment, respectively. The testicular tissue collected was divided into two portions, with one portion being used to measure testicular transferrin (Tf) concentrations by our EIA [6], and the other portion being used for histological examination after staining with PAS-hematoxylin. Germ cell counting was performed by the methods described previously [10] in 5 seminiferous tubules at stage 9 of the seminiferous epithelial cycle. Semen specimens were collected by digital manipulation at one-week intervals and each specimen was evaluated by the methods described previously [9]. Plasma T concentrations in the three dogs peaked on Day 1 after the start of hormone treatment (7.8-12.0 ng/ml), and then decreased to the basal levels (1.4-2.0 ng/ml) on Day 14. The testicular Tf concentrations in all three dogs clearly increased (Table 1) after T-depot plus PMSG treatment, and spermatogenesis in the seminiferous tubules improved (Table 1 and Fig. 2). Semen quality improved between 1 and 5 weeks after the start of T-depot plus PMSG treatment (Fig. 1). It was of particular interest that the number of sperm and the percentages of motile sperm increased every week after each of the three hormone treatments in all three dogs. The percentages of motile sperm in Dogs No. 1 and No. 2 increased from below 50% before hormone treatment to 80% between 3 weeks and 5 weeks after the start of hormone treatment. The percentages of morphologically abnormal sperm, mainly bent or coiled tail, were transiently decreased by this hormone therapy. The plasma T concentration of normal dogs has been The authors have used GnRH-analogue or hCG to treat dogs with low plasma testosterone (T) concentrations and spermatogenic dysfunction, but the semen quality of a few of the treated dogs did not improve at all [7, 8]. Harrop [3] reported fail...

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Changes in Plasma Testosterone and Testicular Transferrin Concentration, Testicular Histology and Semen Quality after Treatment of Testosterone-Depot plus PMSG to 3 Dogs with Asthenozoospermia.

Kawakami

Hori

Tsutsui

2000

The Journal of Veterinary Medical Science

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“…In the subset of dogs with >300 and >600 million spermatozoa in the ejaculate, this correlation persisted (details in Table S4a-b, SI). In samples containing spermatozoa but with low ALP (de ned with the cut-off of <5000 IU [24,25], n=17), the average total sperm count (average 600 × 10 6 ) was lower compared to in samples with ALP >5000 IU (1.3 × 10 9 , p=0.001). Likewise, in samples with low ALP, the concentration was lower (85 × 10 6 ) compared to in samples with high ALP (184 × 10 6 , p=005).…”

Section: Correlation Between Biomarkers and Semen Qualitymentioning

confidence: 99%

Endocrine and dog factors associated with semen quality

Hallberg,

Olsson,

Lau

et al. 2023

Preprint

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Knowledge of factors associated with semen quality may help in investigations of the aetiology and pathophysiology. We investigated the correlation between biomarkers for testicular cell function (anti-müllerian hormone, AMH, Inhibin B, testosterone, steroid-hormone binding globulin, SHBG, insulin like peptide 3, INSL-3), alkaline phosphate (ALP), canine prostate-specific esterase (CPSE), and heterophilic antibodies with dog variables, semen quality, and fertility. Blood and semen were collected from 65 Bernese Mountain Dogs. We evaluated total sperm count, motility and morphological parameters. The semen quality ranged from poor to excellent, with an average total sperm count of 1.1 × 109 and 50% morphologically normal spermatozoa (MNS). Age and abnormal testicular consistency correlated with decreased motility and MNS. Higher ALP correlated with higher total sperm count.. AMH could not be detected in seminal plasma. AMH in blood correlated with head defects and high AMH concentration correlated with a severe decline in several semen parameters. Testosterone was negatively and CPSE positively correlated with age. No correlations were found for INSL-3, inhibin B, or heterophilic antibodies. Our findings contribute to the understanding of factors associated with semen quality in dogs, particularly related to Sertoli cell function.

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“…Medroxyprogesterone acetate reduced testosterone level, but had no significant effect on libido; low doses had no effect on semen quality, high doses decreased semen quality (Bamberg-Thalén & Linde-Forsberg, 1992;England, 1997;Paramo et al, 1993;Wright et al, 1979); megestrol acetate in low doses caused no change in semen quality, higher doses increased minor secondary sperm abnormalities, with no effect on libido (England, 1997); chlormadinone acetate in low doses had no effect on the testes, high doses decreased sperm count and motility, with an increased percentage of abnormal sperm (Kawakami et al, 1993;Murakoshi et al, 1992) Androgens Testosterone enhanced libido, but suppressed spermatogenesis (Freshman et al, 1990); testosterone esters produced a significant decrease in the plasma LH concentration and semen quality (England, 1997;Feldman & Nelson, 2004); synthetic androgen mesterolone improved sperm morphology of an oligoasthenoteratozoospermic dog (England & Allen, 1991) Antiandrogens Cyproterone acetate reduced libido and spermatogenesis (Renggli et al, 2010); osaterone acetate caused only minor reduction of libido and semen quality with transient increase in the percentage of morphologically abnormal spermatozoa (Ferré-Dolcet et al, 2022;Nizanski et al, 2020;Tsutsui et al, 2001); flutamide administrated for 1 year had no adverse effects on libido and sperm production (Romagnoli, 2006), longer administration for two or more years caused testicular interstitial cell hyperplasia, seminiferous tubular atrophy and degeneration (Frank et al, 2004) Estrogenic compounds Ethinylestradiol depressed testosterone level and negatively affected testes and semen quality (Bamberg-Thalén & Linde-Forsberg, 1992), administered for 1 year caused atrophy of the epithelium of the seminiferous tubuli with arrest of spermatogenesis, reduction of size and weight of testes, prostatic squamous metaplasia and prostate enlargement (Zayed et al, 1998); estradiol administered for one year caused atrophy of the epithelium of the seminiferous tubuli with arrest of spermatogenesis, reduction of size and weight of testes, prostatic squamous metaplasia and prostate enlargement (Zayed et al, 1998); diethylstilbestrol impaired spermatogenesis and reduced semen quality (Nizanski et al, 2014) Antioestrogens Clomiphene citrate improved poor semen quality (Kobayashi et al, 2018); tamoxifen reduced libido, ejaculate volume, sperm count and impairment sperm motility and morphology…”

Section: Progestogensmentioning

confidence: 99%

“…Testosterone esters (5 mg/kg) produced a significant decrease in the plasma LH concentration and semen quality (England, 1997). Daily oral administration of 50 mg of methyltestosterone to intact adult male greyhounds for 90 days resulted in decreased mean daily sperm output and mean testicular length (Freshman et al, 1990). The synthetic androgen mesterolone does not significantly suppress the secretion of gonadotrophin and at a dosage 3 mg/kg daily in divided doses significantly improved the sperm morphology of an oligoasthenoteratozoospermic dog (England & Allen, 1991).…”

Section: Hormones and Anti‐hormonal Agentsmentioning

confidence: 99%

Effect of drugs on fertility in male dogs: A review

Zduńczyk

Domosławska

2022

Reprod Domestic Animals

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The aim of this literature review was to present and discuss the available data on the positive and negative effects of drugs on male dog fertility. Apart from information on hormones and anti‐hormonal agents, there is still only little information available regarding the effect of other drugs on sexual function and fertility in male dogs. A negative impact on fertility in male dogs has been reported for vincristine, cyclophosphamide, tetracycline and ketoconazole. However, preclinical safety studies of drugs for human use indicated that spermatogenesis in dogs may be sensitive to a wide variety of drugs. Thus, potential adverse effects of drugs on fertility should always be considered before their use in stud dogs. Also, in cases of reduced fertility or infertility in male dogs, previous medical treatment should be taken into account as a possible cause. In most cases, the effects of drugs on sexual function and spermatogenesis are reversible after the discontinuation of the drug. Further studies on the effects of drugs on male dog fertility are needed.

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The effects of methyltestosteone on reproductive function in male greyhounds

Cited by 10 publications

References 14 publications

Changes in Plasma Testosterone and Testicular Transferrin Concentration, Testicular Histology and Semen Quality after Treatment of Testosterone-Depot plus PMSG to 3 Dogs with Asthenozoospermia.

Changes in Plasma Testosterone and Testicular Transferrin Concentration, Testicular Histology and Semen Quality after Treatment of Testosterone-Depot plus PMSG to 3 Dogs with Asthenozoospermia.

Endocrine and dog factors associated with semen quality

Effect of drugs on fertility in male dogs: A review

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