The Effects of Different Buffers on the Oxidation of DNA by Thiols and Ferric Iron

Interaction of HapX with the CCAAT‐binding complex—a novel mechanism of gene regulation by iron

“…To avoid possible oxidation reactions caused by DTT‐mediated reduction of Fe 3+ in HEPES buffer (Spear and Aust, 1998), the running buffer for analyses of CBC/HapX interaction in the presence of metal salts contained 10 mM phosphate buffer pH 7.4, 2.7 mM KCl, 137 mM NaCl, 1 mM DTT and 0.005%(v/v) surfactant P20. For metal treatment of HapX, freshly prepared stock solutions of FeCl 3 , (NH 4 ) 2 Fe(SO 4 ) 2 or CuCl 2 were used.…”

Selenium-Based Fluorescence Probes for the Detection of Bioactive Molecules

“…1,4-Dithiothreitol (DTT) is a synthetic thiol , and is commonly used as a powerful reducing agent. , DTT is a highly toxic substance which in the presence of transition metals lead to oxidative damage of biomolecules. , It also increases the toxicity of some arsenic and mercury containing compounds. , In 2010, a highly selective fluorescent probe 94 was developed for the detection of DTT using the internal charge transfer (ICT) mechanism …”

Effect of diluent composition on the dynamics of sperm DNA fragmentation and other sperm quality parameters in ram during incubation at 37°C

“…Some buffers are not physiologically inert and may have different effects on cells (Yániz et al, 2012). For example, buffers can act as metal ligands, affecting several reactions necessary for DNA oxidation by ferric iron and thiols, such as iron reduction (Spear and Aust, 1998). A significant correlation between sperm DNA fragmentation and oxidative DNA damage has been described in different studies (Aitken et al, 2010), suggesting a link between the two types of DNA damage and a ROS attack on sperm DNA (Aitken et al, 2010;De Luliis et al, 2009;Thomson et al, 2009;Aitken and Curry, 2011).…”