The effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and ameliorating role of vitamin E and vitamin C

Abstract

Diazinon is one of the most widely used organophosphate insecticides (OPIs) in agriculture and public health programs. Reactive oxygen species (ROS) caused by OPIs may be involved in the toxicity of various pesticides. The aim of this study was to investigate how diazinon affects lipid peroxidation (LPO) and the antioxidant defense system in vivo and the possible ameliorating role of vitamins E and C. For this purpose, experiments were done to study the effects of DI on LPO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in adult rat heart. Experimental groups were: (1) control group, (2) diazinon treated (DI) group, (3) DI+vitamins E and C-treated (DI+Vit) group. The levels of malondialdehyde (MDA) and the activities of SOD and CAT increased significantly in the DI group compared with the control group. The activity of SOD and the levels of MDA decreased significantly in the DI+Vit group compared with the DI group. The differences between the DI+Vit and control groups according to the MDA levels and the activities of both SOD and CAT were statistically significant. These results suggest that treating rats with a single dose of diazinon increases LPO and some antioxidant enzyme activities in the rat myocardium and, in addition, that single-dose treatment with a combination of vitamins E and C after the administration of diazinon can reduce LPO caused by diazinon, though this treatment was not sufficiently effective to reduce the values to those in control group.

Hepatoprotective Impact of Chlorella vulgaris Powder on Deltamethrin Intoxicated Rats

“…This enzyme is sensitive to liver toxicity and its histopathological changes compared with other enzymes as ALP and AST that can be evaluated in a shorter period of time [32]. Another enzyme which is assessed to diagnose liver damage is ALP which exists in most tissues and its level is altered due to liver diseases [33]. ALT and ALP levels were increased significantly (p<0.05) in the current study after deltamethrin intoxication when compared with the control group.…”

Protective Effects of Erdosteine and Vitamins C and E Combination on Ischemia–Reperfusion-Induced Lung Oxidative Stress and Plasma Copper and Zinc Levels in a Rat Hind Limb Model

“…Sahin et al [36] showed that erdosteine treatment in vancomycin-induced liver damage causes significant decrease in SOD, CAT, and GSH-Px activities. Akturk et al [37] studied the effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and ameliorating role of VCE administration. They reported that VCE treatment led to decrease in SOD and CAT activities in diazinon-induced damage of rat heart [37].…”

Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model

“…Other studies found that the increased activity of CAT is due to the adaptive response to the generated free radicals, indicating the failure of the total antioxidant defense mechanism to protect the tissues from mechanical damage caused by pesticides, as evidenced by lipid peroxidation (12)(13)(14). Table 3.…”

“…Vitamin C treatment prevented the enhancement of SOD enzyme activities and enhanced the level of glutathione. Akturk et al (12) studied the effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and the ameliorating roles of vitamin E and vitamin C. Their study found that vitamin E and C treatments led to diminished SOD and CAT activities in diazinon-induced damage of rat heart.…”