The effects of common house fly (Musca domestica) larvaederived substances on wound healing in mouse model

Abstract

Background: Maggot debridement therapy is a therapeutic wound myiasis that depends mainly on L. sericata larva. Owing to its availability and cheap breeding, M. domestica was suggested as an alternative to Lucilia. Objective: The present study was designed to assess the role of M. domestica larvae-derived substances on wound healing in immunocompetent and immunosuppressed mice. Material and Methods: Chitosan and gut extracts, obtained from M. domestica larvae, were applied daily on skin wounds of Swiss albino mice. Mice were divided into two groups, control non-treated mice (I), and experimental treated mice (II). Each group included immunocompetent (a), and immunosuppressed (b) mice. The experimental subgroups were treated with either chitosan (1) or gut extracts (2). Wounds were assessed by macroscopic evaluation, wound contracture, histopathological, immunohistochemical and bacteriological parameters. Results:In control non treated mice hair growth was evident with normal underlying skin by the end of the experiment (4 weeks) except in immunosuppressed (Ib) subgroup. Significant reduction in wound size was detected on the 7 th day post wounding (PW) in the immunocompetent-chitosan-treated subgroup (IIa1) compared to Ia, IIb1, and IIa2 subgroups. Histopathological examination showed early epidermal creeping on the 3 rd day PW in IIa1, IIa2, and IIb2 subgroups. Significant increase in collagen deposition was best detected in both gut extract-treated subgroups (IIa2 and IIb2) compared to the control subgroups (Ia, and Ib). Strong immunohistochemical reaction was evident in all immunocompetent treated mice (IIa1 and IIa2) by the 7 th day PW and in IIb2 by the 14 th day PW. Delay in keratin maturation was detected in both control subgroups and IIb1. Significant reduction in staphylococcal colonies was detected by the 7 th day PW in all immunosuppressed treated subgroups compared to their control subgroup (Ib). Conclusion:The difference in the rapid wound closure as detected by chitosan treatment, and the effective skin architecting by gut extract treatment recommends further trial by their combined therapy.

Zoonotic parasites with significant importance for tropical dermatology

“…A recent report demonstrated the beneficial use of M. domestica as an alternative to other commonly used larvae in maggot debridement therapy being more available and easier to breed. Moreover, it was confirmed that chitosan which is extracted from the cuticles of larvae was superior in accelerating wound closure [24] .…”